A-ACET-HIST H2B(K16)CHIP GRD 100
货号： 07-341 产品名称： A-ACET-HIST H2B(K16)CHIP GRD 100 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-acetyl-Histone H2B (Lys16) Antibody
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REFERENCES
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Suka, N, et al. (2001) Mol. Cell, 8: 473-9 (2001)
Species Reactivity Key Applications Host Format Antibody Type
Yeast (S. cerevisiae) WB Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H2B (Lys16) Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Histone H2B acetylated on lysine 16
Molecular Weight:
15kDa
Immunogen:
peptide APAEAcKKPAAK-C corresponding to amino acids 12-21 of yeast histone H2B
Modifications:
Acetylation
Isotype:
IgG
Species Reactivity:
Yeast (S. cerevisiae)
Quality Assurance:
routinely evaluated by immunoblot on wild-type yeast whole cell lysates as compared to lysates with a substitution mutant of K16R in histone H2B
Purification Method:
Antiserum
Presentation:
antiserum containing 30% glycerol and 0.01% thimerosal
Storage Conditions:
2 years at -20°C
UniProt Number:
Q8N257
Entrez Gene Number:
NP_778225
Gene Symbol:
HIST3H2BB
Alternate Names:
H2BK16Ac
Histone H2B (acetyl K16)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
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UniProt Summary:
FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 126 amino acids; 13908 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 ‘Lys-4’ and ‘Lys-79’ methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermu -20℃
