pUNO1-bTLR2

货号： puno1-btlr2      产品名称： pUNO1-bTLR2   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

TLR2 Native Genes
Toll-Like Receptor 2 native genes in expression plasmid
Alias: CD282
TLR2 is involved in the recognition of a wide array of microbial molecules.
TLR2 recognizes peptidoglycan, lipoteichoic acid and lipoprotein from gram-positive bacteria, lipoarabinomannan from mycobacteria, and zymosan from yeast cell wall.
Moreover, TLR2 participates in the recognition of some types of LPS[1]. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of microbial molecules that TLR2 can recognize. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipopeptide, and associates with TLR1 to recognize triacylated lipopetides [2]. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14 [3].
This gene is available in pUNO1 expression plasmid selectable with Blasticidin or in pUNO3 expression plasmid selectable with Hygromycin or in pUNO1 expression plasmid selectable with Blasticidin.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Bovine TLR2 (pUNO1-bTLR02) Genbank: NM_174197.2 ORF Size: 2355 bp
Subclone: AgeI – NheI
Human TLR2 (pUNO1-hTLR02)
ORF Size: 2355 bp
Subclone: AgeI – NheI
Human TLR2 (pUNO3-hTLR02)
ORF Size: 2355bp
Subclone: AgeI – NheI
Mouse TLR2 (pUNO1-mTLR02) Genbank: AF216289.1 ORF Size: 2355 bp
Subclone: AgeI – NheI
Pig TLR2 (pUNO1-pTLR02)
Genbank: AB208696.2 ORF Size: 2358 bpSubclone: AgeI – NheI

pUNO1-bTLR5

货号： puno1-btlr5      产品名称： pUNO1-bTLR5   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

TLR5 Native Genes
Toll-Like Receptor 5 native genes in expression plasmid
Alias: CD285
TLR5 is the Toll-like molecule that recognizes flagellin from both Gram-positive and Gram-negative bacteria.
TLR5 was identified by the presence of the TIR domain and is expressed in spleen, peripheral blood leukocytes and epithelial cells.
Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1, 2]. TLR5 can generate a proinflammatory signal as a homodimer suggesting that it might be the only TLR required for flagellin recognition [2].
This gene is available in pUNO1 expression plasmid selectable with Blasticidin or in pUNO3 expression plasmid selectable with Hygromycin or in pUNO1 expression plasmid selectable with Blasticidin.
Bovine TLR5 (pUNO1-bTLR05)
ORF Size: 2577 bp
Subclone: NcoI – NheI
Human TLR5 (pUNO1-hTLR05)
ORF Size: 2577 bp
Subclone: NcoI – NheI
Human TLR5 (pUNO3-hTLR05)
ORF Size: 2577bp
Subclone: NcoI – NheI
Mouse TLR5 (pUNO1-mTLR05)
ORF Size: 2577 bp
Subclone: NcoI – NheI
Pig TLR5 (pUNO1-pTLR05)
ORF Size: 2571 bp
Subclone: AgeI – NheI

pUNO1-bTLR6

货号： puno1-btlr6      产品名称： pUNO1-bTLR6   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

TLR6 Native Genes
Toll-Like Receptor 6 native genes in expression plasmid
Alias: CD286
TLR6 is expressed in spleen and BPL and, similarly to TLR1, acts as a co-receptor.
Studies with dominant negative receptors have shown that TLR6 cooperates with TLR2 to recognize peptidoglycan and the yeast cell wall particle, zymosan [1].
Furthermore, TLR6- and TLR2-deficient mice were reported to be hyporesponsive to mycoplasma macrophage-activating lipopeptide-2 kD (MALP-2), a diacylated lipoprotein, suggesting that TLR2 and TLR6 coordinate the response to this ligand. By contrast, TLR2 is able to recognize bacterial lipoproteins triacylated at the N-terminus cysteine residue [2]. Thus TLR6 appears to discriminate between the N-terminal lipoylated structures of MALP-2 and lipopeptides derived from other bacteria.
This gene is available in pUNO1 expression plasmid selectable with Blasticidin or in pUNO3 expression plasmid selectable with Hygromycin or in pUNO1 expression plasmid selectable with Blasticidin.
Bovine TLR6 (pUNO1-bTLR06)
ORF Size: 2391 bp
Subclone: AgeI – NheI
Human TLR6 (pUNO1-hTLR06)
Genbank: NM_006068.4
ORF Size: 2391 bp
Subclone: AgeI – NheI
Human TLR6 (pUNO3-hTLR06)
ORF Size: 2388 bp
Subclone: AgeI – NheI
Mouse TLR6 (pUNO1-mTLR06)
Genbank: AB020808.1
ORF Size: 2418 bp
Subclone: NcoI – NheI
Pig TLR6 (pUNO1-pTLR06)
Genbank: AB210286.1
ORF Size: 2391 bp
Subclone: AgeI – NheI

pUNO1-bTLR7

货号： puno1-btlr7      产品名称： pUNO1-bTLR7   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

TLR7 Native Genes
Toll-Like Receptor 7 native genes in expression plasmid
Alias: CD287
TLR7 is abundantly expressed in lung, placenta, spleen and BPL [1].
TLR7 is phylogenetically close to TLR8 and TLR9 and has a higher molecular weight when compared with hTLR1-6, largely as a result of a longer ectodomain [2].
The natural ligand for TLR7 has not yet been identified. However, studies with TLR7-deficient mice have shown that TLR7 recognizes imidazoquinoline compounds, such as R848, a small synthetic antiviral molecule [3]. Imidazoquinoline signaling involves the MyD88-dependent signaling cascade and induces the production of IFN-α, TNF-α and IL-12.
This gene is available in pUNO1 expression plasmid selectable with Blasticidin or in pUNO3 expression plasmid selectable with Hygromycin or in pUNO1 expression plasmid selectable with Blasticidin.
Bovine TLR7 (pUNO1-bTLR07)
Genbank: NM_001033761.1
ORF Size: 3177 bp
Subclone: AgeI – NheI
Human TLR7 (pUNO1-hTLR07)
ORF Size: 3150 bp
Subclone: AgeI – NheI
Human TLR7 (pUNO3-hTLR07)
ORF Size: 3150 bp
Subclone: AgeI – NheI
Mouse TLR7 (pUNO1-mTLR07)
Genbank: NM_133211.3
ORF Size: 3153 bp
Subclone: AgeI – NheI
Pig TLR7 (pUNO1-pTLR07)
ORF Size: 3153 bp
Subclone: AgeI – NheI

pUNO1-bTLR9

货号： puno1-btlr9      产品名称： pUNO1-bTLR9   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

TLR9 Native Genes
Toll-Like Receptor 9 native genes in expression plasmid
Alias: CD289
TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane [1].
TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation [2].
TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively [3]. TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded [4].
This gene is available in pUNO1 expression plasmid selectable with Blasticidin or in pUNO3 expression plasmid selectable with Hygromycin or in pUNO1 expression plasmid selectable with Blasticidin.
Bovine TLR9 (pUNO1-bTLR09)
Genbank: EF076731
ORF Size: 3090 bp
Subclone: AgeI – NheI
Human TLR9 isoform 1 (pUNO1-hTLR09a)
ORF Size: 3099 bp
Subclone: MluI – NheI
Human TLR9 isoform 1 (pUNO3-hTLR09a)
ORF Size: 3099 bp
Mouse TLR9 (pUNO1-mTLR09)
ORF Size: 3099 bp
Subclone: AgeI – NheI
Pig TLR9 (pUNO1-pTLR09)
Genbank: AY859728.1
ORF Size: 3093 bp
Subclone: AgeI – NheI