Plate Catridge ABS V1, VTX2
货号： 5000-1425 产品名称： Plate Catridge ABS V1, VTX2 品牌： Qiagen 规格： ***
4364014 MNL,GSG ABS QUANT 7900 V2.3 品牌 Invitrogen
MNL,GSG ABS QUANT 7900 V2.3
货号： 4364014 产品名称： MNL,GSG ABS QUANT 7900 V2.3 品牌： Invitrogen 规格： *** 三周到货 生化试验
7900HT Absolute Quantitation Guide
该指南将指导您利用7900HT 快速实时荧光定量PCR系统完成标准曲线（绝对定量）assay的整个制作程序。
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Product Size: 1 manual
1 Manual
ABS102 Anti-AKAP 79 品牌 Millipore
Anti-AKAP 79
货号： ABS102 产品名称： Anti-AKAP 79 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-AKAP 79
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-AKAP 79 Antibody
Promotional Text:
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Specificity:
This antibody recognizes AKAP 79 at the C-terminus.
Molecular Weight:
~79 kDa observed.
AKAP79 migrates in WB with an apparent molecular weight of ~79 kDa. Uncharacterized bands appear at ~35 kDa and ~13 kDa in some lysates.
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of human AKAP 79.
Background Information:
A-kinase anchoring protein 79 (AKAP 79) is a scaffolding protein that resides predominantly in excitatory neuronal synapses. AKAP 79 interacts with membrane-associated guanylate kinase (MAGUK) to be used by glutamate receptors. AKAP 79 may also be involved with SAP97 and other large signaling complexes. AKAP 79 is essential for forskolin-induced up-regulation of L-current.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Control:
HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79
Quality Assurance:
Evaluated by Western Blot in HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79.
Western Blot Analysis: 1 µg/mL of this antibody detected AKAP 79 on 10 µg of HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P24588
Entrez Gene Number:
NP_004848
Gene Symbol:
AKAP5
AKAP79
Alternate Names:
A-kinase anchor protein 5
AKAP-5
A-kinase anchor protein 79 kDa
AKAP 79
H21
cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq].
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UniProt Summary:
FUNCTION: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off th 2-8℃
ABS107 Anti-phospho LRRK2 (Tyr1503) 品牌 Millipore
Anti-phospho LRRK2 (Tyr1503)
货号： ABS107 产品名称： Anti-phospho LRRK2 (Tyr1503) 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-phospho-LRRK2 (Tyr1503)
Species Reactivity Key Applications Host Format Antibody Type
H, Bab, M, R, Po WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-LRRK2 (Tyr1503) Antibody
Promotional Text:
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Specificity:
This antibody recognizes the ROC domain of LRRK2 phosphorylated at Tyr1503.
Molecular Weight:
~200 kDa observed. The observed MW is the truncated wild-type LRRK2 transfected protein.
The calculated molecular weight of endogenous LRRK2 is ~286 kDa.
Epitope:
ROC domain
Immunogen:
KLH-conjugated linear peptide corresponding to the ROC domain of human LRRK2 phosphorylated at Tyr1503.
Modifications:
Phosphorylation
Background Information:
Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.
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Species Reactivity:
Human
Baboon
Mouse
Rat
Pig
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Baboon, Mouse, Rat, Porcine based on 100% sequence homology.
Application Notes:
Peptide Inhibition Analysis: 0.2 µg/mL from representative lot blocked phospho-LRRK2 (Tyr1503) in cell lysates transfected with truncated wild-type LRRK2.
Control:
Cell lysates transfected with truncated forms of wild-type LRRK2 with auto-phosphorylation properties and mutant D1994A LRRK2 which lacks auto-phosphorylation properties.
Quality Assurance:
Evaluated by Western Blot in lysates from cells transfected with truncated forms of wild-type LRRK2 and mutant D1994A LRRK2. D1994A LRRK2 contains a mutation at aspartic acid 1994 which limits its auto-phosphorylation activity.
Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-LRRK2 (Tyr1503) in lysates from cells transfected with truncated wild-type LRRK2, but not in lysates from cells transfected with mutant D1994A LRRK2.
View All »
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q5S007
Entrez Gene Number:
NP_940980
Gene Symbol:
LRRK2
PARK8
Alternate Names:
Leucine-rich repeat serine/threonine-protein kinase 2
Dardarin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex 2-8℃
ABS108 Anti-phospho LRRK2 (Tyr1491) 品牌 Millipore
Anti-phospho LRRK2 (Tyr1491)
货号： ABS108 产品名称： Anti-phospho LRRK2 (Tyr1491) 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-phospho-LRRK2 (Tyr1491)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Po, Bab, B WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-LRRK2 (Tyr1491) Antibody
Promotional Text:
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Specificity:
This antibody recognizes the ROC domain of LRRK2 phosphorylated at Tyr1491.
Molecular Weight:
~200 kDa observed. The observed MW is the truncated wild-type LRRK2 transfected protein.
The calculated molecular weight of endogenous LRRK2 is ~286 kDa.
Epitope:
ROC domain
Immunogen:
KLH-conjugated linear peptide corresponding to the ROC domain of human LRRK2 phosphorylated at Tyr1491.
Modifications:
Phosphorylation
Background Information:
Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.
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Species Reactivity:
Human
Mouse
Rat
Pig
Baboon
Bovine
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Mouse, Rat, Porcine, Baboon, and Bovine based on 100% sequence homology.
Application Notes:
Peptide Inhibition Analysis: 0.2 µg/mL from representative lot blocked phospho-LRRK2 (Tyr1491) in cell lysates transfected with truncated wild-type LRRK2.
Control:
Cell lysates transfected with truncated forms of wild-type LRRK2 with auto-phosphorylation properties and mutant D1994A LRRK2 which lacks auto-phosphorylation properties.
Quality Assurance:
Evaluated by Western Blot in lysates from cells transfected with truncated forms of wild-type LRRK2 and mutant D1994A LRRK2. D1994A LRRK2 contains a mutation at aspartic acid 1994 which limits its auto-phosphorylation activity.
Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-LRRK2 (Tyr1491) in lysates from cells transfected with truncated wild-type LRRK2, but not in lysates from cells transfected with mutant D1994A LRRK2.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q5S007
Entrez Gene Number:
NP_940980
Gene Symbol:
LRRK2
PARK8
Alternate Names:
Leucine-rich repeat serine/threonine-protein kinase 2
Dardarin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, 2-8℃
ABS111 Anti-Fidgetin 品牌 Millipore
Anti-Fidgetin
货号： ABS111 产品名称： Anti-Fidgetin 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Fidgetin
Species Reactivity Key Applications Host Format Antibody Type
M, H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Fidgetin Antibody
Promotional Text:
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100% Performance Guaranteed
Molecular Weight:
~60 kDa observed.
The calculated molecular weight of this protein is 82 kDa, but is observed at ~60 kDa due to a splice variant (Yang, Y., et al. (2005). Experimental Cell Research. 304:50-58).
Uncharacterized bands may be observed at ~25 kDa and ~57 kDa in some tissue lysates.
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Immunogen:
KLH-conjugated linear peptide corresponding to human Fidgetin.
Background Information:
Fidgetin is a AAA protein that belongs to the meitoic or subfamily-7 (SF7) group of ATPases. These enzymes are associated with many different general cellular activities, such as but not limited to, membrane fusion, proteolysis, metabolic signaling, peroxisome biogenesis, endosome sorting, and meiotic spindle formation. However, the specific functions and mechanisms for SF7 AAA proteins remain largely unclear. It is reported that mutant Fidgetin is found in a mammalian developmental mutant and is suggested to play in role in embryonic development.
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Species Reactivity:
Mouse
Human
Species Reactivity Note:
Demonstrated to react with Mouse and Human.
Application Notes:
Western Blot Analysis: 1 µg/mL from a representative lot detected Figetin in U20S cell lysate.
Control:
Mouse brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Figetin in 10 µg of mouse brain tissue lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q5HY92
Entrez Gene Number:
NP_060556
Gene Symbol:
FIGN
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
SUBCELLULAR STRUCTURE: Interacts with AKAP8 (via C-terminus).
SUBCELLULAR LOCATION: Nucleus matrix.
Product Name:
Anti-Fidgetin
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-Fidgetin Antibody is an antibody against Fidgetin for use in WB.
2-8℃
ABS150 Anti-phospho-PDGFRα (Tyr754) 品牌 Millipore
Anti-phospho-PDGFRα (Tyr754)
货号： ABS150 产品名称： Anti-phospho-PDGFRα (Tyr754) 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-phospho-PDGFRα (Tyr754)
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PRODUCT FAMILY INFORMATION
Glial Cell Markers
Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
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Species Reactivity Key Applications Host Format Antibody Type
M, H, R, B WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PDGFRα (Tyr754) Antibody
Promotional Text:
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Specificity:
This antibody recognizes PDGFRα phosphorylated at (Tyr754).
Molecular Weight:
~180 kDa observed
Epitope:
Phosphorylated (Tyr754)
Immunogen:
KLH-conjugated linear peptide corresponding to human PDGFRα phosphorylated at (Tyr754).
Modifications:
Phosphorylation
Background Information:
PDGFRα (platelet-derived growth factor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A) is a ubiquitous receptor tyrosine kinase. It is activated by the binding of homo- or heterodimers of PDGFA, PDGFB or PDGFC. Upon ligand binding, PDGFRα dimerizes as two alpha or two beta subunits depending on the cellular context, and is autophosphorylated at multiple tyrosine residues. Phosphorylated tyrosine residues interact with different targets to mediate cell proliferation, migration, and survival. Phosphorylation at Tyrosine 754 induces heterodimerization and allows PDGFRα to interact with PTPN11. In addition, PDGRα regulates apoptosis by multiple signaling pathways that converge on proapoptotic proteins such as BAD. For instance, PDGFRα signaling deactivates BAD by induction of PI3K/Akt; PLC-gamma/PKC/Bcl-2; and Ras/Raf/Erk signaling pathways. Defective PDGFRα has been implicated in hypereosinophilic syndrome and gastrointestinal stromal tumor.
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Species Reactivity:
Mouse
Human
Rat
Bovine
Species Reactivity Note:
Demonstrated to react with Mouse. Predicted to react with Human, Rat, and Bovine based on 100% sequence homology. Other homologies: Xenopus and Chicken (91% sequence homology).
Control:
Untreated and PDGF treated NIH/3T3 cell lysates
Quality Assurance:
Evaluated by Western Blot in untreated and PDGF treated NIH/3T3 cell lysates.
Western Blot Analysis: 2.5 µg/mL of this antibody detected PDGFRα on 10 µg of untreated and PDGF treated NIH/3T3 cell lysates.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P16234
Entrez Gene Number:
NP_006197
Gene Symbol:
PDGFRA
PDGFR2
RHEPDGFRA
Alternate Names:
Platelet-derived growth factor receptor alpha
PDGF-R-alpha
PDGFR-alpha
Alpha platelet-derived growth factor receptor
Alpha-type platelet-derived growth factor receptor
CD140 antigen-like family member A
CD140a antigen
Platelet-derived growth factor alpha receptor
Platelet-derived growth factor receptor 2
Short name=PDGFR-2
CD_antigen=CD140a
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), ou 2-8℃
ABS157 Anti-SKAR 品牌 Millipore
Anti-SKAR
货号： ABS157 产品名称： Anti-SKAR 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-SKAR
Species Reactivity Key Applications Host Format Antibody Type
H, R WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-SKAR Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Molecular Weight:
~51 kDa observed. An uncharacterized band appears at ~90 kDa in some lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human SKAR.
Background Information:
SKAR (S6K1 Aly/REF-like substrate) is a nuclear protein that is localized at exon junction complexes (EJC) which interact with pre-mRNA molecules to ensure that correct splicing has occurred. SKAR recruits ribosomal protein S6 kinase 1 (S6K1) to EJCs and links S6K1 to the mTOR pathway. Although the function of SKAR has not been elucidated, previous studies suggest that it plays an important role in the quality control machinery that checks newly synthesized mRNA for errors and assembles the translation apparatus, thereby regulating the synthesis of new proteins and possible cell growth.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Demonstrated to react with Human and Rat. Other homologies: Mouse (93% sequence homology).
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SKAR in HeLa cells.
Control:
Raji cell lysate
Quality Assurance:
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected SKAR on 10 µg of Raji cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9BY77
Entrez Gene Number:
NP_115687
Gene Symbol:
POLDIP3
PDIP46
Alternate Names:
Polymerase delta-interacting protein 3
46 kDa DNA polymerase delta interaction protein
p46
S6K1 Aly/REF-like target
SKAR
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq, Jul 2008].
UniProt Summary:
FUNCTION: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
SUBUNIT STRUCTURE: Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH.
SUBCELULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note: Nucleocytoplasmic shuttling protein.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated at Ser-383 and Ser-385 by RPS6KB1.
2-8℃
ABS159 Anti-HPK1 品牌 Millipore
Anti-HPK1
货号： ABS159 产品名称： Anti-HPK1 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-HPK1
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REFERENCES
Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Hu, M C, et al. (1996) Genes Dev., 10: 2251-64 (1996)
Species Reactivity Key Applications Host Format Antibody Type
H WB, IH(P) Rabbit Purified Polyclonal Antibody
Description:
Anti-HPK1 Antibody
Promotional Text:
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100% Performance Guaranteed
Specificity:
This antibody recognizes HPK1 at the N-terminus.
Molecular Weight:
~97kDa observed. The calculated molecular weight is 91 kDa, however HPK1 has been shown as a ~97 kDa band in western blots. (Hu, M., et al. (1996). Genes Dev. 10:2251-2264.)
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of human HPK1.
Background Information:
Hematopoietic progenitor kinase 1 (HPK1), otherwise known as MEKKK 1 and MAP4K1, is a serine/threonine kinase belonging to the Ste20 (Sterile-20) family. It is predominantly expressed in hematopoietic cells and is involved in sigaling pathways requiring MAP kinase activation; HPK1 directly upstream of MEKK1. HPK1 forms signaling complexes with a number of adaptor proteins including Nck, SLP-76, and Grb2 family of proteins; these proteins may facilitate the tethering of HPK1 to membrane receptors such as the epidermal growth factor receptor. However, HPK1 may also interact with adaptor proteins to induce transcription. For example, interaction with CRKL, and Crk adaptor proteins activates the c-Jun N-terminal kinase (JNK) pathway leading to gene expression. HPK1 may mediate cell growth, cytokine signaling, and cell death.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Other homologies: Mouse and Rat (86% sequence homoloogy).
Application Notes:
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected HPK1 in human tonsil tissue.
Control:
Raji cell lysate
Quality Assurance:
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected HPK1 on 10 µg of Raji cell lysate.
Purification Method:
Protein A purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q92918
Entrez Gene Number:
NP_009112
Gene Symbol:
MAP4K1
HPK1
Alternate Names:
Mitogen-activated protein kinase kinase kinase kinase 1
Hematopoietic progenitor kinase
MAPK/ERK kinase kinase kinase 1
MEK kinase kinase 1
MEKKK 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
SUBUNIT STRUCTURE: Interacts with MAP3K1.
TISSUE SPECIFICITY: Expressed primarily in hematopoietic 2-8℃
ABS162 Anti-Apolipoprotein H 品牌 Millipore
Anti-Apolipoprotein H
货号： ABS162 产品名称： Anti-Apolipoprotein H 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Apolipoprotein H
Recommended Replacement for: AB788
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Apolipoprotein H Antibody
Replaces:
AB788
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes the Sushi 4 domain of Apolipoprotein H.
Molecular Weight:
~50 kDa observed
Epitope:
Sushi 4 domain
Immunogen:
KLH-conjugated linear peptide corresponding to the Sushi 4 domain of human Apolipoprotein H.
Background Information:
Also known as Native β2-glycoprotein 1, Apolipoprotein H is a carbohydrate-rich plasma protein which is partially comprised of sialylated and galactosylated bi- and triantennary oligosaccharides and is expressed by the liver and secreted in plasma. It is reported that Apolipoprotein H is an initiating antigen that is required for antibody recognition and is also known to bind to anionic phospholipids. It is also suggested to play a role in lipid metabolism.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Bovine, Canine, Rhesus Macaque, Chimpanzee, Giant Panda, Equine, Porcine, and Orangutan based on 100% sequence homology.
Control:
Human plasma
Quality Assurance:
Evaluated by Western Blot in human plasma.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Apolipoprotein H on 10 µg of human plasma.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P02749
Entrez Gene Number:
NP_000033
Gene Symbol:
APOH
B2G1
Alternate Names:
Beta-2-glycoprotein 1
APC inhibitor
Activated protein C-binding protein
Anticardiolipin cofactor
Apolipoprotein H
Apo-H
Beta-2-glycoprotein I
B2GPI
Beta(2)GPI
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Product Name:
Anti-Apolipoprotein H
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-Apolipoprotein H Antibody detects level of Apolipoprotein H & has been published & validated for use in Western Blotting.
2-8℃
ABS166 Anti-Arap3 品牌 Millipore
Anti-Arap3
货号： ABS166 产品名称： Anti-Arap3 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Arap3 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Arap3 Antibody
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Molecular Weight:
~170 kDa observed. An uncharacterized band appears at ~75 kDa in some lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human Arap3.
Background Information:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 (ARAP3) is a ubiquitous GTPase activating protein (GAP). ARAP3 is activated by either phosphatidylinositol 3,4,5-trisphosphate or phosphatidylinositol 3,4-bisphosphate. It regulates RhoA, ARF6, and RAC1 GTPases involved in the remodeling of the actin cytoskeleton.
Species Reactivity:
Human
Control:
Huvec cell lysate
Quality Assurance:
Evaluated by Western Blot in Huvec cell lysate.
Western Blot Analysis: 4 µg/mL of this antibody detected Arap3 on 10 µg of Huvec cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q8WWN8
Entrez Gene Number:
NP_071926
Gene Symbol:
ARAP3
CENTD3
Alternate Names:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
Centaurin-delta-3
Cnt-d3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Product Name:
Anti-Arap3 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-Arap3 detects levels of Arap3 proteins & has been published & validated for use in WB.
2-8℃
ABS179 Anti-ADAP1 品牌 Millipore
Anti-ADAP1
货号： ABS179 产品名称： Anti-ADAP1 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-ADAP1
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-ADAP1 Antibody
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Specificity:
This antibody recognizes the C-terminus of ADAP1.
Molecular Weight:
~44 kDa observed
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of human ADAP1.
Background Information:
Arf-GAP with dual PH domain-containing protein 1 (ADAP1), also known as p42(IP4), PIP3BP, or centaurin-alpha1, is a highly abundant brain protein that has been detected in the hippocampus, cerebellum, cortex, and hypothalamus. It is an actin-binding protein that has been localized to the nucleus, cytoplasm, plasma membrane, and mitochondria. ADAP1 contains a zinc finger motif and two pleckstrin homology domains. It functions as a receptor for inositol(1,3,4,5)tetrakisphosphate and phosphatidylinositol(3,4,5)trisphosphate second messengers, and also binds to and becomes phosphorylated by all isoforms of PKC. It has been linked to calcium transport in the mitochondria and RNA metabolism in the nucleus. In addition, it has been shown that ADAP1 modulates the development and differentiation of dendrites in hippocampal cells, as it is involved in the formation of dendritic filopodia and spine-like protrusions. It has also been reported that ADAP1 is upregulated in Alzheimer’s disease.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat.
Application Notes:
Immunohistochemistry Analysis: A representative lot detected ADAP1 in normal mouse cerebellum and hippocampus tissues.
Western Blot Analysis: A representative lot detected ADAP1 in human brain tissue lysate.
Control:
Mouse brain tissue lysate.
Quality Assurance:
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 mg/mL of this antibody detected ADAP1 in 10 µg of mouse brain tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O75689
Entrez Gene Number:
NP_006860
Gene Symbol:
ADAP1
CENTA1
Alternate Names:
Arf-GAP with dual PH domain-containing protein 1
Centaurin-alpha-1
Cnt-a1
Putative MAPK-activating protein PM25
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Product Name:
Anti-ADAP1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
This Anti-ADAP1 Antibody is validated for use in Western Blotting, IHC(P) for the detection of ADAP1.
2-8℃
ABS180 Anti-PDE4B1 品牌 Millipore
Anti-PDE4B1
货号： ABS180 产品名称： Anti-PDE4B1 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-PDE4B1
Species Reactivity Key Applications Host Format Antibody Type
M WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PDE4B1 Antibody
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Specificity:
This antibody recognizes PDE4B1 near the N-terminus.
Molecular Weight:
~104 kDa observed. UniProtKB describes 3 isoforms produced by alternative splicing: 83, 64, and 82 kDa. PDE4B1 and PDE4B3 isoforms have a molecular mass approximately 20 kDa larger based on their mobility in SDS-PAGE. (Huston, E., et al. (1997). Biochem. J. 328:549-558.)
Epitope:
Near N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human PDE4B1 near the N-terminus.
Background Information:
Phosphodiesterase 4B1 (PDE4B1) is a member of the PDE cyclic nucleotides. PDE4B1 is thought to contain both UCR1 and UCR2 regulatory units. PDE4B1 has been associated with schizophrenia and depression and could be a promising target for autism therapies.PDE4B1 created a doublet with PDE4B3, which increases in the presence of high levels of cAMP.
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Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with Mouse. Expected to react with human based on immunogen design.
Control:
Mouse brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in Mouse brain tissue lysate.
aWestern Blot Analysis: 1 µg/mL of this antibody detected PDE4B1 on 10 µg of Mouse brain tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q07343
Entrez Gene Number:
NP_001032418
Gene Symbol:
PDE4B
DPDE4
Alternate Names:
cAMP-specific 3′,5′-cyclic phosphodiesterase 4B
DPDE4
PDE32
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
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UniProt Summary:
FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
CATALYTIC ACTIVITY: Adenosine 3′,5′-cyclic phosphate + H2O = adenosine 5′-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind z 2-8℃
ABS181 PDE4B2-N4B2 品牌 Millipore
PDE4B2-N4B2
货号： ABS181 产品名称： PDE4B2-N4B2 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-PDE4B2 Anti-AMPylated Tyrosine
货号： ABS184 产品名称： Anti-AMPylated Tyrosine 品牌： Millipore 规格： EA 三周到货 生化实验 Anti-AMPylated Tyrosine -20℃ Anti-PIP5K-Type 1 gamma
货号： ABS190 产品名称： Anti-PIP5K-Type 1 gamma 品牌： Millipore 规格： EA 三周到货 生化实验 Anti-PIP5K-Type 1 gamma 2-8℃ Anti-phospho PDHE1-A type I (Ser300)
货号： ABS194 产品名称： Anti-phospho PDHE1-A type I (Ser300) 品牌： Millipore 规格： EA 三周到货 生化实验 Anti-phospho PDHE1-A type I (Ser300) Anti-Haptoglobin-Related Protein
货号： ABS196 产品名称： Anti-Haptoglobin-Related Protein 品牌： Millipore 规格： EA 三周到货 生化实验 Anti-Haptoglobin-Related Protein Anti-Spred-2
货号： ABS198 产品名称： Anti-Spred-2 品牌： Millipore 规格： EA 三周到货 生化实验 Anti-Spred-2 Anti-phospho-PHDE1-A type I (Ser293)
货号： ABS204 产品名称： Anti-phospho-PHDE1-A type I (Ser293) 品牌： Millipore 规格： EA 三周到货 生化实验 Anti-phospho-PDHE1-A type I (Ser293)
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Species Reactivity Key Applications Host Format Antibody Type
R WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PDE4B2 Antibody
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Specificity:
This antibody recognizes PDE4B2 at the N-terminus.
Molecular Weight:
~78 kDa observed. UniProt describes 3 isoforms produced by alternative splicing: Isoform PDE4B1 at 83.3 kDa, Isoform PDE4B2 at 64.5 kDa, and Isoform PDE4B3 at 82.1 kDa.
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of human PDE4B2.
Background Information:
Phosphodiesterase 4B2 (PDE4B2) is a member of the PDE cyclic nucleotides. PDE4B2 is thought to contain both UCR1 and UCR2 regulatory units. PDE4B2 has been associated with schizophrenia and depression and could be a promising target for autism therapies.
Species Reactivity:
Rat
Species Reactivity Note:
Demonstrated to react with Rat. Predicted to react with Human based on immunogen design.
Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in Rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PDE4B2 on 10 µg of Rat brain membrane tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q07343
Entrez Gene Number:
NP_001032416
Gene Symbol:
PDE4B
DPDE4
Alternate Names:
cAMP-specific 3′,5′-cyclic phosphodiesterase 4B
DPDE4
PDE32
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
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UniProt Summary:
FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
CATALYTIC ACTIVITY: Adenosine 3′,5′-cyclic phosphate + H2O = adenosine 5′-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by rolipram.
ABS184 Anti-AMPylated Tyrosine 品牌 Millipore
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Serum Polyclonal Antibody
Description:
Anti-AMPylated Tyrosine Antibody
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Specificity:
This antibody recognizes AMPylated Tyrosines.
Molecular Weight:
~21 kDa observed. Molecular weights will vary based on the protein being AMPylated.
Isotype:
IgG
Background Information:
AMPylation is a reversible post-translational modification in which adenosine monophosphate (AMP) derived from ATP is added to threonine and tyrosine (and possibly serine) residues on target proteins. This modification is created by proteins containing Fic (filamentation induced by cyclic adenosine monophosphate) domains, which are present in both prokaryotic and eukaryotic cells. In mammals, the HYPE protein is the most widely known Fic-containing protein and the precise function of its AMPylated targets is not yet clear. However, bacterial cells possess numerous Fic-domain proteins that modify and disable protein targets in host cells, thereby suppressing signaling pathways and increasing the robustness of pathogenic infections. The Vibrio parahaemolyticus VopS protein, for instance, disrupts the function of Rho guanine triphosphatases, and eventually disrupts the actin cytoskeleton.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Broad species reactivity expected, as AMPylation is evolutionarily conserved.
Control:
Rac1, AMPylated in vitro
Quality Assurance:
Evaluated by Western Blot in Rac1, AMPylated in vitro.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected AMPylated Tyrosine on 10 ng of Rac1, AMPylated in vitro.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Alternate Names:
AMPylated Tyrosine
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Product Name:
Anti-AMPylated Tyrosine
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Serum
Host:
Rabbit
Applications:
Anti-AMPylated Tyrosine Antibody detects level of AMPylated Tyrosine & has been published & validated for use in Western Blotting.ABS190 Anti-PIP5K-Type 1 gamma 品牌 Millipore
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REFERENCES
PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse.
Wenk, M R, et al. (2001) Neuron, 32: 79-88 (2001)
Species Reactivity Key Applications Host Format Antibody Type
M, R WB, IHC Rabbit Purified Polyclonal Antibody
Description:
Anti-PIP5K-Type 1 gamma Antibody
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Molecular Weight:
~90 kDa observed. The calculated molecular weight of PIP5K Type-1 gamma is 69-72 kDa, but has been observed at ~90 kDa in Western Blot (Volpicelli-Daley, L.A., et al. (2010). 285(37):28708-28714.).
Immunogen:
recombinant protein corresponding to mouse PIP5K-type 1 gamma.
Isotype:
IgG1
Background Information:
Phosphatidylinositol-4-phosphate 5-kinase 1 gamma (PIP5K type 1 gamma; PIP5K1γ) is a ubiquitous protein which is enriched in focal adhesion plaques. It is expressed in relatively high levels in the brain, particularly at synaptic sites. PIP5K type 1 gamma is activated by talin, and in turn phosphorylates phosphatidylinositol 4-phosphate to form phosphatidylinositol 4,5-bisphosphate, the precursor to the second messenger phosphatidylinositol 3,4,5-trisphosphate. It indirectly influences vesicle transport, cell adhesion, and cell migration, among other processes. Defects in PIP5K type 1 gamma may contribute to lethal congenital arthrogryposis.
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Species Reactivity:
Mouse
Rat
Application Notes:
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PIP5K-Type 1 gamma in rat brain tissues (Wenk, M. R., et al. 2001. Neuron. 32(1):79-88.).
Control:
Mouse brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 mg/mL of this antibody detected PIP5K-Type 1 gamma in 10 mg of mouse brain tissue lysate.
Purification Method:
Protein A purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O70161
Entrez Gene Number:
NP_001140159
Gene Symbol:
Pip5k1c
Alternate Names:
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
PIP5K1-gamma
PtdIns(4)P-5-kinase 1 gamma
Phosphatidylinositol 4-phosphate 5-kinase type I gamma
PIP5KIgamma
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-PIP5K-Type 1 gamma
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rabbit
Applications:
Anti-PIP5K-Type 1 gamma Antibody detects level of PIP5K-Type 1 gamma & has been published & validated for use in Western Blotting & IHC.ABS194 Anti-phospho PDHE1-A type I (Ser300) 品牌 Millipore
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, B, Xn WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho PDHE1-A type I (Ser300) Antibody
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Specificity:
This antibody recognizes PDHE1-A type I phosphorylated at Ser300.
Molecular Weight:
~43 kDa observed
Epitope:
Phosphorylated Ser300
Immunogen:
KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.
Modifications:
Phosphorylation
Background Information:
In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
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Species Reactivity:
Human
Mouse
Rat
Bovine
Xenopus
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Zebra Fish, and Xenopus based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.
Control:
Dichloroacetate untreated and treated HEK 293 cell lysates
Quality Assurance:
Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P08559
Entrez Gene Number:
NP_000275
Gene Symbol:
PDHA1
PHE1A
Alternate Names:
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
PDHE1-A type I
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key rol 2-8℃ ABS196 Anti-Haptoglobin-Related Protein 品牌 Millipore
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REFERENCES
Towards an understanding of adsorption behaviour in non-aqueous systems: adsorption of poly(vinyl pyrrolidone) and poly(ethylene glycol) onto silica from 2H, 3H-perfluoropentane.
Alison Paul,Peter C Griffiths,Philippe G Rogueda (2005) The Journal of pharmacy and pharmacology.57
Species Reactivity Key Applications Host Format Antibody Type
H, Chp WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Haptoglobin-Related Protein Antibody
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Specificity:
This antibody recognizes the Peptidase S1 domain of Haptoglobin-related protein.
Molecular Weight:
~39 kDa observed.
A band at a molecular weight of ~92 kDa due to dimerization may be observed in human serum (Smith, A.B., et al. (1995). Proc Natl Acad Sci USA. 92(22):10262-10266).
Epitope:
Peptidase S1 domain
Immunogen:
KLH-conjugated linear peptide corresponding to the Peptidase S1 domain of human Haptoglobin-Related Protein.
Background Information:
It is reported that trypanosome lytic factor (TRF) consists of a haptoglobin-related protein that serves as a toxic substance against trypanosomes. This constituent is a protein that is known to be a toxic subtype of HDLs. When bound to hemoglobin, it is shown to kill trypanosomes through its peroxidative activity. It is also suggested to serve as a biomarker for early stage Alzheimer’s disease and may play a role in lipid metabolism and weight regulation.
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Species Reactivity:
Human
Chimpanzee
Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Chimpanzee based on 100% sequence homology.
Application Notes:
Multiplex Assay Analysis: 100 ng/mL from a representative lot detected Haptoglobin-Related Protein.
Control:
Human serum
Quality Assurance:
Evaluated by Western Blot in human serum.
Western Blot Analysis: 50 ng/mL of antibody detected Haptoglobin-Related Protein in 10 µg of human serum.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P00739
Entrez Gene Number:
NP_066275
Gene Symbol:
HPR
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: In adult liver the amount of HPR mRNA is at the lower limit of detection, therefore the extent of its expression is at most less than 1000-fold that of the HP1F gene. No HPR mRNA can be detected in fetal liver. Expressed in hepatoma G2 and leukemia molt-4 cell lines.
SEQUENCE SIMILARITIES: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 1 Sushi (CCP/SCR) domain.
CAUTION: Although homologous to serine proteases, it has lost all essential catalytic residues and has no enzymatic activity.
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Product Name:
Anti-Haptoglobin-Related Protein
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concent 2-8℃ ABS198 Anti-Spred-2 品牌 Millipore
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Spred-2 Antibody
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Molecular Weight:
~50 kDa observed.
An uncharacterized band may be observed at ~38 kDa in some cell lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human Spred-2.
Background Information:
The Sprouty/Spread family of proteins contains three members, which share high sequence homology for the cysteine-rich domain (CRD), and are shown to inhibit Ras/ERK signaling. Along with its other family members, Spred-2 inhibits Ras/MAP kinase activity (mediated by the epidermal growth factor receptor and the fibroblast growth factor receptor) by binding to Ras and thereby preventing Raf phosphorylation. Like Spred-1, Spred-2 competes with substrates Tau and STAT3 for the DYRK1a kinase domain and thereby inhibits DYRK1a substrate phosphorylation. It is reported that Spred-2 appears to be involved in cytoplasmic vesicle transport and in other metabolic signaling.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Other homologies: Mouse and Rat (89% sequence homology).
Control:
PC-3 cell lysate
Quality Assurance:
Evaluated by Western Blot in PC-3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Spred-2 in 10 µg of PC-3 cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q7Z698
Entrez Gene Number:
NP_001121682
Gene Symbol:
SPRED2
Alternate Names:
Sprouty-related, EVH1 domain-containing protein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
SPRED2 is a member of the Sprouty (see Entrez Gene website for SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see Entrez Gene website MAPK1; MIM 176948). (Nonami, A., et al. (2004). 279(50):52543-52551.)
UniProt Summary:
FUNCTION: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
SUBUNIT STRUCTURE: Interacts with Ras. Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle > secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.
TISSUE SPECIFICITY: Expressed in liver, skin, small intestine, salivary gland and prostate.
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Product Name:
Anti-Spred-2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
This Anti-Spre 2-8℃ ABS204 Anti-phospho-PHDE1-A type I (Ser293) 品牌 Millipore
Species Reactivity Key Applications Host Format Antibody Type
H, R, M, B, Pm WB, ICC, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PDHE1-A type I (Ser293) Antibody
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Specificity:
This antibody recognizes PDHE1-A type I phosphorylated at Ser293.
Molecular Weight:
~43 kDa observed
Epitope:
Phosphorylated Ser293
Immunogen:
KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser293.
Modifications:
Phosphorylation
Background Information:
In many organisms, the pyruvate dehydrogenase (PDH) complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic pathway. This complex also serves as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1 or PDHE1-A type I), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
Species Reactivity:
Human
Rat
Mouse
Bovine
Primate
Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Rat, Mouse, Bovine, Zebra Fish, and Primate based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cells.
Control:
Untreated and DCA-treated HEK293 cell lysates
Quality Assurance:
Evaluated by Western Blot in DCA untreated and treated HEK293 cell lysates.
Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cell lysates.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P08559
Entrez Gene Number:
NP_000275
Gene Symbol:
PDHA1
PHE1A
Alternate Names:
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Immunocytochemistry
Immunoprecipitation
Entrez Gene Summary:
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced t 2-8℃