Anti-Spred-2

货号： ABS198      产品名称： Anti-Spred-2   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Spred-2
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Spred-2 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Molecular Weight:
~50 kDa observed.
An uncharacterized band may be observed at ~38 kDa in some cell lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human Spred-2.
Background Information:
The Sprouty/Spread family of proteins contains three members, which share high sequence homology for the cysteine-rich domain (CRD), and are shown to inhibit Ras/ERK signaling. Along with its other family members, Spred-2 inhibits Ras/MAP kinase activity (mediated by the epidermal growth factor receptor and the fibroblast growth factor receptor) by binding to Ras and thereby preventing Raf phosphorylation. Like Spred-1, Spred-2 competes with substrates Tau and STAT3 for the DYRK1a kinase domain and thereby inhibits DYRK1a substrate phosphorylation. It is reported that Spred-2 appears to be involved in cytoplasmic vesicle transport and in other metabolic signaling.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Other homologies: Mouse and Rat (89% sequence homology).
Control:
PC-3 cell lysate
Quality Assurance:
Evaluated by Western Blot in PC-3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Spred-2 in 10 µg of PC-3 cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q7Z698
Entrez Gene Number:
NP_001121682
Gene Symbol:
SPRED2
Alternate Names:
Sprouty-related, EVH1 domain-containing protein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
SPRED2 is a member of the Sprouty (see Entrez Gene website for SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see Entrez Gene website MAPK1; MIM 176948). (Nonami, A., et al. (2004). 279(50):52543-52551.)
UniProt Summary:
FUNCTION: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
SUBUNIT STRUCTURE: Interacts with Ras. Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle > secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.
TISSUE SPECIFICITY: Expressed in liver, skin, small intestine, salivary gland and prostate.
View All »
Product Name:
Anti-Spred-2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
This Anti-Spre 2-8℃