Anti-phospho LRRK2 (Tyr1503)

货号： ABS107      产品名称： Anti-phospho LRRK2 (Tyr1503)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-LRRK2 (Tyr1503)
Species Reactivity Key Applications Host Format Antibody Type
H, Bab, M, R, Po  WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-LRRK2 (Tyr1503) Antibody
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Specificity:
This antibody recognizes the ROC domain of LRRK2 phosphorylated at Tyr1503.
Molecular Weight:
~200 kDa observed. The observed MW is the truncated wild-type LRRK2 transfected protein.
The calculated molecular weight of endogenous LRRK2 is ~286 kDa.
Epitope:
ROC domain
Immunogen:
KLH-conjugated linear peptide corresponding to the ROC domain of human LRRK2 phosphorylated at Tyr1503.
Modifications:
Phosphorylation
Background Information:
Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.
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Species Reactivity:
Human
Baboon
Mouse
Rat
Pig
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Baboon, Mouse, Rat, Porcine based on 100% sequence homology.
Application Notes:
Peptide Inhibition Analysis: 0.2 µg/mL from representative lot blocked phospho-LRRK2 (Tyr1503) in cell lysates transfected with truncated wild-type LRRK2.
Control:
Cell lysates transfected with truncated forms of wild-type LRRK2 with auto-phosphorylation properties and mutant D1994A LRRK2 which lacks auto-phosphorylation properties.
Quality Assurance:
Evaluated by Western Blot in lysates from cells transfected with truncated forms of wild-type LRRK2 and mutant D1994A LRRK2. D1994A LRRK2 contains a mutation at aspartic acid 1994 which limits its auto-phosphorylation activity.
Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-LRRK2 (Tyr1503) in lysates from cells transfected with truncated wild-type LRRK2, but not in lysates from cells transfected with mutant D1994A LRRK2.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q5S007
Entrez Gene Number:
NP_940980
Gene Symbol:
LRRK2
PARK8
Alternate Names:
Leucine-rich repeat serine/threonine-protein kinase 2
Dardarin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex 2-8℃