标签归档：pdgfr

V3721   PDGFRα Kinase Enzyme System   品牌 Promega

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PDGFRα Kinase Enzyme System

货号： V3721      产品名称： PDGFRα Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在Sf9 昆虫细胞中，我们通过杆状病毒表达N 端带GST 标签的重组人源PDGFRα （第550 位氨基酸 – 末端）。 PDGFRα （血小板来源的生长因子受体α ）是具有内源酪氨酸激酶活性的PDGFR 膜受体家族中的一员。PDGFRα 的异常表达与模式脊椎动物的发育异常相关，也与人类的多种疾病状态相关。在肾纤维化和硬化的病变过程中，PDGFRα 在不同类型的肾脏细胞中广泛表达。在肠炎修复阶段，PDGF 与其受体PDGFRα 是纤维化的诱导因子，它们也可能参与了活跃的炎症期。
ADP-Glo™ Kinase Assay（ADP-Glo™激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的ADP 含量：ADP 转化为ATP 后，ATP 即可作为Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成ADP 的酶类( 如激酶或ATP 酶 ) 的活性，ATP浓度可高达1mM 。
激酶酶系统的组成：
•  激酶：PDGFRα ，10μg ( 人源， 重组 ； 第550 位氨基酸 – 末端 ) ；MW: ~ 95kDa 。
•  底物：Poly (4:1 Glu, Tyr) Peptide 。
•  其它组分：反应缓冲液，DTT 。
PDGFRα NCBI 数据库入口。
人类激酶酶系统及其应用。
特点  •  在实验室内即可筛选更多的化合物：ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。•  
•  完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的DTT 和MnCl2 。
•  可得到可靠结果：ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 
 

 

V3731   PDGFRβ Kinase Enzyme System   品牌 Promega

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PDGFRβ Kinase Enzyme System

货号： V3731      产品名称： PDGFRβ Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在Sf9 昆虫细胞中，我们通过杆状病毒表达N 端带GST 标签的重组人源PDGFRβ （第557 位氨基酸 – 末端）。PDGFRβ （ 血小板来源的生长因子受体β ）是PDGFR 膜受体家族中的一员，具有内源酪氨酸激酶活性。PDGFRβ 缺失的小鼠易出血、严重贫血、并表现出肾小球功能缺陷。然而PDGFRβ 的缺失对主要血管及心脏并没有影响。在一些癌症中，PDGFRβ 的表达量与活性都会升高；在动物模型中，PDGFRβ 活性的抑制阻断了肾癌的扩散。
ADP-Glo™ Kinase Assay（ADP-Glo™激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的ADP 含量：ADP 转化为ATP 后，ATP 即可作为Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成ADP 的酶类( 如激酶或ATP 酶 ) 的活性，ATP浓度可高达1mM 。
激酶酶系统的组成：
•  激酶：PDGFRβ ，10μg ( 人源，重组， 第557 位氨基酸 – 末端 ) ；MW : ~ 104 kDa 。
•  底物：Poly (4:1 Glu, Tyr) Peptide 。
•  其它组分：反应缓冲液，DTT 。
PDGFRβ NCBI 数据库入口。
人类激酶酶系统及其应用。 
特点  •  在实验室内即可筛选更多的化合物：ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。•  
•  完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的DTT 和MnCl2 。
•  可得到可靠结果：ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 
 

7770-5   PDGFR beta, Active   品牌 Biovision

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PDGFR beta, Active

货号： 7770-5      产品名称： PDGFR beta, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active PDGFR beta
Important factors regulating cell proliferation, cellular differentiation, cell growth and development
Product Overview
Alternate Names / Synonyms: Active PDGFR beta, PDGFR beta, PDGFR, human PDGFR beta, active kinase, active kinases, recombinant PDGFR beta, recombinant PDGFR, kinase activity assay, protein kinase
Gene Symbol: PDGFRB
Accession #: NM_002609.
Gene ID: 5159
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Molecular Weight: 96.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: The specific activity of PDGFRb was determined to be 20 nmol /min/mg as per activity assay protocol.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: PDGFRb (platelet-derived growth factor receptor b) is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity. PDGFRb deficient mice are hemorrhagic, severely anemic and exhibit a defect in kidney glomeruli function (1). However, absence of PDGFRb has no impact on major blood vessels and the heart. PDGFRb expression and activity is elevated in several cancers and inhibition of PDGFRb activity blocks progression of renal carcinoma in an animal model (2).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

ABS150   Anti-phospho-PDGFRα (Tyr754)   品牌 Millipore

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Anti-phospho-PDGFRα (Tyr754)

货号： ABS150      产品名称： Anti-phospho-PDGFRα (Tyr754)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PDGFRα (Tyr754)
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PRODUCT FAMILY INFORMATION
Glial Cell Markers
Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
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Species Reactivity Key Applications Host Format Antibody Type
M, H, R, B  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PDGFRα (Tyr754) Antibody
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Specificity:
This antibody recognizes PDGFRα phosphorylated at (Tyr754).
Molecular Weight:
~180 kDa observed
Epitope:
Phosphorylated (Tyr754)
Immunogen:
KLH-conjugated linear peptide corresponding to human PDGFRα phosphorylated at (Tyr754).
Modifications:
Phosphorylation
Background Information:
PDGFRα (platelet-derived growth factor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A) is a ubiquitous receptor tyrosine kinase. It is activated by the binding of homo- or heterodimers of PDGFA, PDGFB or PDGFC. Upon ligand binding, PDGFRα dimerizes as two alpha or two beta subunits depending on the cellular context, and is autophosphorylated at multiple tyrosine residues. Phosphorylated tyrosine residues interact with different targets to mediate cell proliferation, migration, and survival. Phosphorylation at Tyrosine 754 induces heterodimerization and allows PDGFRα to interact with PTPN11. In addition, PDGRα regulates apoptosis by multiple signaling pathways that converge on proapoptotic proteins such as BAD. For instance, PDGFRα signaling deactivates BAD by induction of PI3K/Akt; PLC-gamma/PKC/Bcl-2; and Ras/Raf/Erk signaling pathways. Defective PDGFRα has been implicated in  hypereosinophilic syndrome and gastrointestinal stromal tumor.
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Species Reactivity:
Mouse
Human
Rat
Bovine
Species Reactivity Note:
Demonstrated to react with Mouse. Predicted to react with Human, Rat, and Bovine based on 100% sequence homology. Other homologies: Xenopus and Chicken (91% sequence homology).
Control:
Untreated and PDGF treated NIH/3T3 cell lysates
Quality Assurance:
Evaluated by Western Blot in untreated and PDGF treated NIH/3T3 cell lysates.
Western Blot Analysis: 2.5 µg/mL of this antibody detected PDGFRα on 10 µg of untreated and PDGF treated NIH/3T3 cell lysates.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P16234
Entrez Gene Number:
NP_006197
Gene Symbol:
PDGFRA
PDGFR2
RHEPDGFRA
Alternate Names:
Platelet-derived growth factor receptor alpha
PDGF-R-alpha
PDGFR-alpha
Alpha platelet-derived growth factor receptor
Alpha-type platelet-derived growth factor receptor
CD140 antigen-like family member A
CD140a antigen
Platelet-derived growth factor alpha receptor
Platelet-derived growth factor receptor 2
Short name=PDGFR-2
CD_antigen=CD140a
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), ou 2-8℃