Anti-phospho PDHE1-A type I (Ser300)

货号： ABS194      产品名称： Anti-phospho PDHE1-A type I (Ser300)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho PDHE1-A type I (Ser300)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, B, Xn  WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho PDHE1-A type I (Ser300) Antibody
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Specificity:
This antibody recognizes PDHE1-A type I phosphorylated at Ser300.
Molecular Weight:
~43 kDa observed
Epitope:
Phosphorylated Ser300
Immunogen:
KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.
Modifications:
Phosphorylation
Background Information:
In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
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Species Reactivity:
Human
Mouse
Rat
Bovine
Xenopus
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Zebra Fish, and Xenopus based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.
Control:
Dichloroacetate untreated and treated HEK 293 cell lysates
Quality Assurance:
Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P08559
Entrez Gene Number:
NP_000275
Gene Symbol:
PDHA1
PHE1A
Alternate Names:
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
PDHE1-A type I
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key rol 2-8℃