标签归档：pipk

ST1517-100UG   ANTI-PIP5K3 MOUSE MAB (6C7)   品牌 Merck

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ANTI-PIP5K3 MOUSE MAB (6C7)

货号： ST1517-100UG      产品名称： ANTI-PIP5K3 MOUSE MAB (6C7)   品牌： Merck 规格： *** 3周到货 生化实验

Anti-PIP5K3 Mouse mAb (6C7)
Anti-Phosphatidylinosi-3-Phosphate-Phosphatidylinosi-5-Kinase Type 3 
Recognizes the ~60 kDa PIP5K3 protein in human testes tissue and HeLa cells.
产品信息
Host  Mouse
Isotype  IgG2a
Immunogen  A recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein
Clone  6C7
Form  liquid
Formulation  In PBS, pH 7.2.
Species reactivity  human
Preservative  None
Applications 
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Avoid freeze/thaw  是的
Positive control  Human testes tissue, HeLa cells
Negative control  293T cells
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Regulatory Review

-20°C

ABS190   Anti-PIP5K-Type 1 gamma   品牌 Millipore

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Anti-PIP5K-Type 1 gamma

货号： ABS190      产品名称： Anti-PIP5K-Type 1 gamma   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-PIP5K-Type 1 gamma
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REFERENCES
PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse.
Wenk, M R, et al. (2001) Neuron, 32: 79-88 (2001)
Species Reactivity Key Applications Host Format Antibody Type
M, R  WB, IHC Rabbit Purified Polyclonal Antibody
Description:
Anti-PIP5K-Type 1 gamma Antibody
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Molecular Weight:
~90 kDa observed. The calculated molecular weight of PIP5K Type-1 gamma is 69-72 kDa, but has been observed at ~90 kDa in Western Blot (Volpicelli-Daley, L.A., et al. (2010). 285(37):28708-28714.).
Immunogen:
recombinant protein corresponding to mouse PIP5K-type 1 gamma.
Isotype:
IgG1
Background Information:
Phosphatidylinositol-4-phosphate 5-kinase 1 gamma (PIP5K type 1 gamma; PIP5K1γ) is a ubiquitous protein which is enriched in focal adhesion plaques. It is expressed in relatively high levels in the brain, particularly at synaptic sites. PIP5K type 1 gamma is activated by talin, and in turn phosphorylates phosphatidylinositol 4-phosphate to form phosphatidylinositol 4,5-bisphosphate, the precursor to the second messenger phosphatidylinositol 3,4,5-trisphosphate. It indirectly influences vesicle transport, cell adhesion, and cell migration, among other processes. Defects in PIP5K type 1 gamma may contribute to lethal congenital arthrogryposis.
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Species Reactivity:
Mouse
Rat
Application Notes:
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PIP5K-Type 1 gamma in rat brain tissues (Wenk, M. R., et al. 2001. Neuron. 32(1):79-88.).
Control:
Mouse brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 mg/mL of this antibody detected PIP5K-Type 1 gamma in 10 mg of mouse brain tissue lysate.
Purification Method:
Protein A purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O70161
Entrez Gene Number:
NP_001140159
Gene Symbol:
Pip5k1c
Alternate Names:
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
PIP5K1-gamma
PtdIns(4)P-5-kinase 1 gamma
Phosphatidylinositol 4-phosphate 5-kinase type I gamma
PIP5KIgamma
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-PIP5K-Type 1 gamma
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rabbit
Applications:
Anti-PIP5K-Type 1 gamma Antibody detects level of PIP5K-Type 1 gamma & has been published & validated for use in Western Blotting & IHC.

2-8℃

07-2132   Anti-PIP5K1 gamma (long form)   品牌 Millipore

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Anti-PIP5K1 gamma (long form)

货号： 07-2132      产品名称： Anti-PIP5K1 gamma (long form)   品牌： Millipore 规格： *** 3周到货 生化实验

Anti-PIP5K1 gamma Antibody, (long form)
Description:
Anti-PIP5K1 gamma Antibody, (long form)
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes PIP5K1 gamma at the C-terminus.
Molecular Weight:
~80 kDa observed. The calculated molecular weight is ~72 kDa.
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of mouse PIP5K1 gamma.
Background Information:
Phosphatidylinositol-4-phosphate 5-kinase 1 gamma (PIP5K1γ) phosphorylates phosphatidylinositol 4-phosphate to create phosphatidylinositol-4,5-bisphosphate and is mainly located in the brain, specifically in the synapses. PIP5K1γ interacts with talin and may play a role in regulating cell adhesion. A defect in PIP5K1γ usually leads to lethal congenital arthrogryposis.
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Species Reactivity:
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Mouse and Rat.
Control:
Mouse brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PIP5K1 gamma on 10 µg of mouse brain tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O70161
Entrez Gene Number:
NP_032870
Gene Symbol:
Pip5k1c
PIP5K1γ
Alternate Names:
Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma
PIP5K1-gamma
PtdIns(4)P-5-kinase 1 gamma
Phosphatidylinositol-4-phosphate 5-kinase type I gamma
PIP5KIgamma
PIP5
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
ENZYME REGULATION: Activated by phosphatidic acid.
SUBUNIT STRUCTURE: Interacts with ARF6 (By similarity). Interacts with TLN1, TLN2 and CSK. Interacts with AP2B1. Isoform 1 interacts with AP2M1; phosphorylation of PIP5K1C by CSK disrupts the interaction; clathrin competes with PIP5K1C.
SUBCELLULAR LOCATION: Endomembrane system. Cell junction › focal adhesion. Note: Associated with membranes. Detected at focal adhesions.
TISSUE SPECIFICTY: Detected in brain, lung, thymus, heart, testicle, kidney and embryo. Highly expressed in forebrain, in particular in cerebellum, hippocampus and cerebral cortex.
PTM: Phosphorylation on Tyr-644 enhances binding to TLN2 and is necessary for targeting to focal adhesions.
SEQUENCE SIMILARITIES: Contains 1 PIPK domain.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:
KM=37 µM for PtdIns4P
KM=39 µM for ATP
SEQUENCE CAUTION: The sequence BAC65601.2 differs from that shown. Reason: Erroneous initiat 2°C – 8°C