标签归档：ship

565835-10MG   SHIP1 Inhibitor, 3AC   品牌 Merck

发表于由

SHIP1 Inhibitor, 3AC

货号： 565835-10MG      产品名称： SHIP1 Inhibitor, 3AC   品牌： Merck 规格： *** 3周到货 生化实验

SHIP1 Inhibitor, 3AC
3α-Aminocholestane, SH2-domain-containing inositol 5′-phosphatase 1 (SHIP1) Inhibitor, 3AC
A cell-permeable steroidal compound that selectively inhibits SHIP1′ polyphosphatase activity toward PI(3,4,5)P3 (IC50 = 10 µM), but not SHIP2 5′ or PTEN′ polyphosphatase activity toward PI(3,4,5)P3 (IC50 > 1 mM). Effectively inhibits SHIP1-mediated immune response both in vitro and in vivo, as well as SHIP1-dependent cancer cell survival. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
产品信息
Form  White solid
Molar mass  387.7
Packaged under inert gas  是的
Purity  ≥98% by 1H NMR
Solubility  EtOH
Chemical formula  C27H49N
商店和运输信息
Storage  +2°C to +8°C
Ship  Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling

2°C – 8°C

565840-10MG   SHIP2 Inhibitor, AS1938909   品牌 Merck

发表于由

SHIP2 Inhibitor, AS1938909

货号： 565840-10MG      产品名称： SHIP2 Inhibitor, AS1938909   品牌： Merck 规格： *** 3周到货 生化实验

SHIP2 Inhibitor, AS1938909
3-(2,4-Dichlorobenzyl)oxy]-N-(2,6-difluorobenzyl)thiophene-2-carboxamide, SH2 domain-containing inositol 5′-phosphatase 2 (SHIP2) Inhibitor, AS1938909
A cell-permeable thiophenecarboxamide compound that is shown to increase glucose metabolism and activate intracellular insulin signaling. Acts as a potent, competitive and reversible inhibitor of SHIP2 activity (Ki = 0.44 µM for hSHIP2) with moderate to excellent selectivity over SHIP1 and other related phosphatases (IC50 = 0.18, 0.57, 21, > 50, > 50 and > 50 µM for mSHIP2, hSHIP2, hSHIP1, hPTEN, h-synaptojanin and h-myotubularin, respectively). Elevates insulin-induced pAkt-Ser473 levels and enhances glucose transporter GLUT1 mRNA expression in L6 myotubes. SHIP1 Inhibitor, 3AC is also available (Cat. No. 565835).
产品信息
Form  Off-white solid
Molar mass  428.3
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥99% by HPLC
Solubility  DMSO
Chemical formula  C19H13Cl2F2NO2S
商店和运输信息
Storage  +2°C to +8°C
Ship  Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling

2°C – 8°C

05-1911   Anti-SHIP, clone P1C1   品牌 Millipore

发表于由

Anti-SHIP, clone P1C1

货号： 05-1911      产品名称： Anti-SHIP, clone P1C1   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-SHIP Antibody, clone P1C1
Description:
Anti-SHIP Antibody, clone P1C1
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes SHIP.
Molecular Weight:
145 kDa observed. Doublet expected at higher antibody concentrations.
Epitope:
Unknown
Immunogen:
KLH-conjugated linear peptide corresponding to human SHIP.
Clone:
P1C1
Isotype:
IgG1κ
Background Information:
Expressed only in hematopoietic cells, Src homology (SH)-2 containing Inositol phosphatase (SHIP) is a member of the Inositol polyphosphatase-5-phosphatase (INPP5) family characterized as containing several protein interaction domains that include an Inositol phosphatase domain, a SH2 domain, and a proline-rich domain. SHIP hydrolyzes the phosphatidylinositol 3-kinase product phosphatidylinositol-3,4,5-triphosphatase, a requirement of activation for several enzymes containing pleckstrin homology domains. The movement of this protein from cytosol to the plasma membrane is regulated by tyrosine phosphorylation as a response to various activations by cytokines and T and B cell receptors. SHIP is a necessary negative regulator, moderating the balance of both the positive and negative signals in myeloid cells and lymphocytes. There are multiple forms of SHIP, which have been shown to result from the truncation of the C-terminus.
View All »
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Application Notes:
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Lucas, D., et al. (1999). Blood. 93(6):1922-1933.)
Control:
THP-1 cell lysate
Quality Assurance:
Evaluated by Western Blot in THP-1 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected SHIP on 10 µg of THP-1 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q92835
Entrez Gene Number:
NP_001017915.1
Gene Symbol:
INPP5D
hp51CN
p150Ship
SHIP
SIP-145
SHIP-1
SHIP1
View All »
Alternate Names:
inositol polyphosphate-5-phosphatase, 145kDa
SH2 containing inositol phosphatase
signaling inositol polyphosphate 5 phosphatase SIP-145
Inositol polyphosphate-5-phosphatase of 145 kDa
inositol polyphosphate-5-phosphatase, 145kD
SH2 domain-containing inositol phosphatase 1
SH2 containing inositol phosphatase, isoform b
SH2 domain-containing inositol-5′-phosphatase 1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunoprecipitation
Entrez Gene Summary:
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement 2°C – 8°C

07-134   ANTI-SHIP2 PC 12-303   品牌 Millipore

发表于由

ANTI-SHIP2 PC 12-303

货号： 07-134      产品名称： ANTI-SHIP2 PC 12-303   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-SHIP2 Antibody
Close
REFERENCES
Ergopeptines bromocriptine and ergovaline and the dopamine type-2 receptor inhibitor domperidone inhibit bovine equilibrative nucleoside transporter 1-like activity.
Edwena D Miles,Yan Xue,James R Strickland,James A Boling,James C Matthews (2011) Journal of agricultural and food chemistry.59
A novel SH2-containing phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase (SHIP2) is constitutively tyrosine phosphorylated and associated with src homologous and collagen gene (SHC) in chronic myelogenous leukemia progenitor cells.
Wisniewski, D, et al. (1999) Blood, 93: 2707-20 (1999)
The two SH2-domain-containing inositol 5-phosphatases SHIP1 and SHIP2 are coexpressed in human T lymphocytes.
Bruyns, C, et al. (1999) Biol. Chem., 380: 969-74 (1999)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Serum Polyclonal Antibody
Description:
Anti-SHIP2 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
SHIP2
Molecular Weight:
120kDa
Immunogen:
Peptide corresponding to residues 1-20 of human SHIP2
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Purification Method:
Antiserum
Presentation:
antiserum containing 0.05% sodium azide and 30% glycerol
Storage Conditions:
2 years at -20°C
UniProt Number:
O15357
Entrez Gene Number:
NM_001567
Gene Symbol:
INPPL1
SHIP2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
INPPL1 encodes inositol polyphosphate-5 phosphatase-like 1, a protein that in addition to the phosphatase domain contains an SH2 (src-homology domain 2) motif.
UniProt Summary:
FUNCTION: SwissProt: O15357 # Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol- 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submenbraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling. Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pat -20°C

ABS10   Anti-SHIP   品牌 Millipore

发表于由

Anti-SHIP

货号： ABS10      产品名称： Anti-SHIP   品牌： Millipore 规格： *** 三周到货 生化实验

Anti-SHIP
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-SHIP Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes SHIP at the C-terminus.
Molecular Weight:
~145 kDa observed
Epitope:
C-terminus
Immunogen:
GST-tagged recombinant protein corresponding to the C-terminus of human SHIP.
Background Information:
Expressed only in hematopoietic cells, Src homology (SH)-2 containing Inositol phosphatase (SHIP) is a member of the Inositol polyphosphatase-5-phosphatase (INPP5) family characterized as containing several protein interaction domains that include an Inositol phosphatase domain, a SH2 domain, and a proline-rich domain. SHIP hydrolyzes the phosphatidylinositol 3-kinase product phosphatidylinositol-3,4,5-triphosphatase, a requirement of activation for several enzymes containing pleckstrin homology domains. The movement of this protein from cytosol to the plasma membrane is regulated by tyrosine phosphorylation as a response to various activations by cytokines and T and B cell receptors. SHIP is a necessary negative regulator, moderating the balance of both the positive and negative signals in myeloid cells and lymphocytes. There are multiple forms of SHIP, which have been shown to result from the truncation of the C-terminus.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated SHIP protein from Ramos lysate.
Control:
TF-1 cell lysate
Quality Assurance:
Evaluated by Western Blot in TF-1 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected SHIP on 10 µg of TF-1 cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q92835
Entrez Gene Number:
NP_001017915
Gene Symbol:
INPP5D
hp51CN
p150Ship
SHIP
SIP-145
SHIP-1
SHIP1
View All »
Alternate Names:
inositol polyphosphate-5-phosphatase, 145kDa
SH2 containing inositol phosphatase
signaling inositol polyphosphate 5 phosphatase SIP-145
Inositol polyphosphate-5-phosphatase of 145 kDa
inositol polyphosphate-5-phosphatase, 145kD
SH2 domain-containing inositol phosphatase 1
SH2 containing inositol phosphatase, isoform b
SH2 domain-containing inositol-5′-phosphatase 1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At 2-8℃