Anti-phospho-ATR (Ser428)

货号： ABE389      产品名称： Anti-phospho-ATR (Ser428)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-ATR (Ser428)
Species Reactivity Key Applications Host Format Antibody Type
H, Mk  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-ATR (Ser428) Antibody
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Specificity:
This antibody recognizes ATR phosphorylated at (Ser428).
Molecular Weight:
~260 kDa observed
Epitope:
Phosphorylated (Ser428)
Immunogen:
KLH-conjugated linear peptide corresponding to human ATR phosphorylated at (Ser428).
Background Information:
The Ataxia-Telangiectasia and Rad3 related kinase (ATR) is a nuclear serine/threonine kinase that is part of the "first-response" system to DNA damage induced by a wide variety of factors including double-stranded breaks and replication stress. The localization of ATR to sites of DNA damage is aided by the Replication Protein A (RPA). Phospho – ATR (Ser428) targets a number of proteins including BRCA1, WRN, CHEK1, MCM2, and p53/TP53, and coordinates signaling pathways involved in DNA repair and apoptosis. ATR may also be involved in the phosphorylation of the histone H2A.X after replication stress, an early marker of DNA damage. However, ATR also mediates DNA replication and fork stability in normal cell cycles by activation of Chk1. Defective ATR contributes to Seckel syndrome type 1.
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Species Reactivity:
Human
Monkey
Species Reactivity Note:
Demonstrated to react in Human and Monkey. Other homologies: (63% mouse sequence homology).
Control:
UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates
Quality Assurance:
Evaluated by Western Blot in UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates.
Western Blot Analysis: A 1:200 dilution of this antibody detected ATR on 10 µg of UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13535
Entrez Gene Number:
NP_001175
Gene Symbol:
ATR
FRP1
Alternate Names:
Serine/threonine-protein kinase ATR
Ataxia telangiectasia and Rad3-related protein
FRAP-related protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
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UniProt Summary 2-8℃