Anti-ZEB1

货号： ABN285      产品名称： Anti-ZEB1   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-ZEB1 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-ZEB1 Antibody
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Molecular Weight:
~200 kDa observed. The calculated molecular weight is 124 kDa, however ZEB1 has been shown as a ~200 kDa band in western blots (Feng, W.H., et al. (2007). Journal of Virology. 81(18):10113-10122). An uncharacterized band may appear at ~240 kDa in some lysates.
Immunogen:
GST-tagged recombinant protein corresponding to human ZEB1.
Background Information:
ZEB1 (zinc-finger-enhancer binding protein 1) is a member of the Zinc finger-Homeobox (ZFH)-family of repressors and acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. ZEB1 is expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Mutations in ZEB1 have been associated with posterior polymorphous corneal dystrophy type 3 which is a rare disease involving metaplasia and overgrowth of corneal endothelial cells.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ZEB1 in HeLa cells.
Control:
HeLa nuclear extract
Quality Assurance:
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZEB1 in 10 µg of HeLa nuclear extract.
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P37275
Gene Symbol:
ZEB1
AREB6
TCF8
Alternate Names:
Zinc finger E-box-binding homeobox 1
NIL-2-A zinc finger protein
Negative regulator of IL2
Transcription factor 8
TCF-8
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Product Name:
Anti-ZEB1 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
150 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
This ZEB1 antibody is validated for use in WB & ICC for the detection of the ZEB1 protein.

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