标签归档：histone

2233-01   Histone H3 Peptide-Biotin Conjugate   品牌 Biovision

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Histone H3 Peptide-Biotin Conjugate

货号： 2233-01      产品名称： Histone H3 Peptide-Biotin Conjugate   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Histone H3 Peptide-Biotin Conjugate
Product Overview
Product Highlights:
•SOURCE- Synthetic
•PURITY- >98% by HPLC
•MOLECULAR WEIGHT- 2916.1
•FORM- Lyophilized without additives
•RECONSTITUTION- Centrifuge the vial prior to opening. Reconstitute in 1 ml/mg dH₂O.
•KEY APPLICATIONS- The suggested applications include Immunoprecipitation, affinity precipitation, and as substrate for histone modified enzymes, etc.
Description: Synthetic peptide corresponding to amino acids 21-44 of human histone H3 followed by a glycine linker and a biotinylated lysine (ATKAARKSAPATGGVKKPHRYRPG-GK-biotin). Due to the nature of the chemistry, the C-terminus will be amidated.
Storage Temp.: -20°C
Shipping: gel pac
Usage: For Research Use Only! Not For Use in Humans.

-20°C

2233-1   Histone H3 Peptide-Biotin Conjugate   品牌 Biovision

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Histone H3 Peptide-Biotin Conjugate

货号： 2233-1      产品名称： Histone H3 Peptide-Biotin Conjugate   品牌： Biovision 规格： 1 mg 3周到货 生化实验

Histone H3 Peptide-Biotin Conjugate
Product Overview
Product Highlights:
•SOURCE- Synthetic
•PURITY- >98% by HPLC
•MOLECULAR WEIGHT- 2916.1
•FORM- Lyophilized without additives
•RECONSTITUTION- Centrifuge the vial prior to opening. Reconstitute in 1 ml/mg dH₂O.
•KEY APPLICATIONS- The suggested applications include Immunoprecipitation, affinity precipitation, and as substrate for histone modified enzymes, etc.
Description: Synthetic peptide corresponding to amino acids 21-44 of human histone H3 followed by a glycine linker and a biotinylated lysine (ATKAARKSAPATGGVKKPHRYRPG-GK-biotin). Due to the nature of the chemistry, the C-terminus will be amidated.
Storage Temp.: -20°C
Shipping: gel pac
Usage: For Research Use Only! Not For Use in Humans.

-20°C

BML-P106-0001   H1-7 (Histone H1 phosphorylation site)   品牌 Biomol

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H1-7 (Histone H1 phosphorylation site)

货号： BML-P106-0001      产品名称： H1-7 (Histone H1 phosphorylation site)   品牌： Biomol 规格： 1 mg 3周到货 生化实验

Product Specification
Alternative Name:Histone H1 phosphorylation site, PKA substrate, Arg-Arg-Lys-Ala-Ser-Gly-Pro, RRKASGP
Sequence:Arg-Arg-Lys-Ala-Ser-Gly-Pro
MW:770.9
CAS:65189-70-0
Purity:97%
Long Term Storage:-20°C

-20°C

BML-P256-0500   Histone H3 Dimethyl Lysine-4 Peptide   品牌 Biomol

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Histone H3 Dimethyl Lysine-4 Peptide

货号： BML-P256-0500      产品名称： Histone H3 Dimethyl Lysine-4 Peptide   品牌： Biomol 规格： 0.5 mg 3周到货 生化实验

Residues 1-21 from the N-terminal tail of human histone H3, dimethylated on the side-chain (ε-amino function) of Lys4. Useful as a substrate for histone lysine demethylases, for example LSD1.
Product Specification
Sequence:Ala-Arg-Thr-Lys(Me2) Gln-Thr-Ala-Arg-Lys-Ser-Thr-Gly-Gly-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala- NH2
MW:2281.5
Purity:95%
Appearance:Lyophilized solid.
Solubility:Soluble in Water.
Long Term Storage:-20°C

-20°C

BML-P271-0500   Histone H3 Peptide   品牌 Biomol

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Histone H3 Peptide

货号： BML-P271-0500      产品名称： Histone H3 Peptide   品牌： Biomol 规格： 0.5 mg 3周到货 生化实验

Product Specification
Sequence:Ac-Gln-Thr-Ala-Arg-Lys-Ser-Thr-Gly-Gly-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala-Thr-Lys-NH2
MW:2068.4
Purity:95%
Appearance:Lyophilized solid.
Solubility:Soluble in Water.
Long Term Storage:-20°C

-20°C

382175-1MG   Histone Deacetylase Inhibitor VIII   品牌 Merck

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Histone Deacetylase Inhibitor VIII

货号： 382175-1MG      产品名称： Histone Deacetylase Inhibitor VIII   品牌： Merck 规格： *** 3周到货 生化实验

Histone Deacetylase Inhibitor VIII
A cell-permeable, active site Zn2+-targeting phenylisoxazolo-hydroxamate that acts a potent and highly selective HDAC6 inhibitor (IC50 = 0.002, 0.42, 90.7, 252, 271 and 6851 nM against HDAC-6, -3, -10, -2, -1 and -8, respectively). Shown to be more potent than SAHA in inhibiting the proliferation of a panel of pancreatic cancer cell lines (IC50 <1.0 µM).
产品信息
Form  Beige to light brown solid
Molar mass  446.5
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥95% by HPLC
Solubility  DMSO
Chemical formula  C22H30N4O6
CAS number  1045792-66-2
商店和运输信息
Storage  +2°C to +8°C
Ship  Ambient Temperature Only
Standard Handling

2°C – 8°C

BML-SE145-0100   Histone Deacetylase 8 (human) (recombinant)   品牌 Biomol

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Histone Deacetylase 8 (human) (recombinant)

货号： BML-SE145-0100      产品名称： Histone Deacetylase 8 (human) (recombinant)   品牌： Biomol 规格： 100 U 3周到货 生化实验

HDAC8 (human), (recombinant)
377 amino acids; HDAC8 from human cDNA. Sequence identical to that at Genbank accession #NM018486.
Product Specification
Alternative Name:Histone deacetylase 8
MW:42 kDa
Source:Produced in E. coli.
UniProt ID:Q9BY41
Formulation:Liquid. In 10mM TRIS, pH 7.5, containing 100mM NaCl, 3mM MgCl2 and 10% glycerol.
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
Biological Activity:Like other class I HDACs, HDAC8 exhibits trichostatin A-inhibitable histone deacetylase activity and can mediate transcription repression.
Specific Activity:≥0.4 U/µg. One unit is defined as the amount of enzyme that deacetylates 1pmol/min of Fluor de Lys™-SIRT1 (Prod. No. BML-KI177) substrate at 37°C / 500µM.
Application:Study HDAC8 kinetics and inhibitor sensitivity and also the effect of the enzyme in transcriptional regulation, cell cycle progression, and oncogenesis.
Long Term Storage:-80°C
Use/Stability:The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best results will be obtained by adding the chilled, undiluted enzyme directly to prewarmed buffer and proceeding immediately to the addition of pre-warmed substrate. Either add a small volume of undiluted enzyme to each well (e.g. 1 U per well) or dilute into pre-warmed buffer (e.g. 1 U per 25 μl) and immediately aliquot to wells (see attached assay conditions). The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC8 into separate tubes and store at -70°C.
NOTE: When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity.

-80°C

382115-10MG   Histone Acetyl Transferase Inhibitor VII, CTK7A   品牌 Merck

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Histone Acetyl Transferase Inhibitor VII, CTK7A

货号： 382115-10MG      产品名称： Histone Acetyl Transferase Inhibitor VII, CTK7A   品牌： Merck 规格： *** 3周到货 生化实验

Histone Acetyl Transferase Inhibitor VII, CTK7A
A cell-permeable and water soluble hydrazinobenzoylcurcumin (HBC) compound that acts as a selective, reversible and mixed mode inhibitor of p300/CBP and PCAF histone acetyltransferases (IC50 ≤ 25 and 50 µM, respectively) with minimal activity towards other histone modifying enzymes like, G9a, CARM1, HDAC1, SIRT2 and TIP60 (IC50 > 100 µM). Blocks the autoacetylation of p300 and PCAF and histone acetylation, downregulates cyclin E expression and induces senescence-like growth arrest at 100 µM in KB cells. Further, suppresses tumor growth in xenograft oral tumor nude mouse model (100 mg/kg, i.p., b.i.d.) and reduces H3K14 and H3K9 acetylation levels. Reported to potently inhibit bFGF-induced bovine aortic endothelial cells (BAECs) invasion and tube formation with an IC50 of 0.93 µM and binds to Ca2+/calmodulin with high-affinity (Kd = 8.11 µM) and antagonize its functions in HCT15 cells.
产品信息
Form  Tan powder
Molar mass  506.5
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥99% by HPLC
Solubility  DMSO
Chemical formula  C28H23N2NaO6
商店和运输信息
Storage  -20°C
Ship  Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling

-20°C

03-185   Histone H2B human recombinant   品牌 Millipore

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Histone H2B human recombinant

货号： 03-185      产品名称： Histone H2B human recombinant   品牌： Millipore 规格： EA 3周到货 生化实验

Histone H2B human recombinant
Description:
Histone H2B human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Human recombinant H2B expressed in E. Coli
Background Information:
Histone H2B is one of four core histones that form an octameric structure required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability and consequently, transcription, and DNA replication and repair. The most widely studied of these modifications are methylation, phosphorylation, and acetylation which may produce different effects on gene regulation depending on the number and pattern of modifications. Histone H2B undergoes acetylation at lysines 5, 15, and 20. It is also phosphorylated in apoptotic cells during DNA fragmentation, possibly in a caspase-dependent manner.
View All »
Applications:
Human recombinant H2B expressed in E. Coli.
Key Applications:
Kinase Assay
DNA-binding Proteins
Western Blotting
Application Notes:
Chromatin assembly, Western blot (control), Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Species:
Human
Molecular Weight:
~17 kDa
Brand Family:
Upstate
Presentation:
Liquid in Dulbecco’s PBS
Storage Conditions:
Stable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.
Product Name:
Histone H2B human recombinant
Physical Form:
Frozen solution
Format:
Purified
Host:
E. coli
Purity:
Purity >95% by SDS-PAGE

-80°C

06-1359   Anti-acetyl-Histone H1 (Lys46).   品牌 Millipore

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Anti-acetyl-Histone H1 (Lys46).

货号： 06-1359      产品名称： Anti-acetyl-Histone H1 (Lys46).   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-acetyl-Histone H1 (Lys46)
Species Reactivity Key Applications Host Format Antibody Type
B  WB, DB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetyl-Histone H1 (Lys46) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Histone H1 acetylated at Lys46.
Molecular Weight:
~33 kDa observed
Epitope:
Acetylated Lys46
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H1 acetylated at Lys46.
Modifications:
Acetylation
Background Information:
H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.
View All »
Species Reactivity:
Bovine
Species Reactivity Note:
Demonstrated to react with Bovine. Broad species reactivity expected based on sequence similarity.
Application Notes:
Dot Blot (Specificity) Analysis: A representative lot blocked acetyl-Histone H1 (Lys46) in unmodified and modified Histones. Possible cross-reactivity with core histones may be observed.
Control:
Calf thymus Histone H1 extract
Quality Assurance:
Evaluated by Western Blot in calf thymus Histone H1 extract.
Western Blot Analysis: A 1:100 dilution of this antibody detected Histone H1 on 10 µg of calf thymus Histone H1 extract.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P10412
Entrez Gene Number:
NP_005312
Gene Symbol:
HIST1H1E
H1F4
Alternate Names:
Histone H1.4
Histone H1b
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Dot Blot
Product Name:
Anti-acetyl-Histone H1 (Lys46)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
Use Anti-acetyl-Histone H1 (Lys46) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect acetyl-Histone H1 (Lys46) also known as Histone H1.4, Histone H1b, H1K46Ac.

2°C – 8°C

06-1370   Anti-phospho-Histone H1 (Thr165)   品牌 Millipore

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Anti-phospho-Histone H1 (Thr165)

货号： 06-1370      产品名称： Anti-phospho-Histone H1 (Thr165)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-Histone H1 (Thr165)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, DB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-Histone H1 (Thr165) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Histone H1 phosphorylated at Thr165.
Molecular Weight:
~33 kDa observed. A cross-reacting band is observed at ~110 kDa.
Epitope:
Phosphorylated Thr165
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H1 phosphorylated at Thr165.
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Broad species crossreactivity due to conserved homology.
Application Notes:
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-phospho-Histone H1 (Thr165) (1:5,000 dilution).
Control:
Untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein
Quality Assurance:
Evaluated by Western Blot in untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 on 10 µg of untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein.
View All »
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P16403
Entrez Gene Number:
NP_005310
Gene Symbol:
HIST1H1C
H1F2
Alternate Names:
Histone H1.2
Histone H1d
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Dot Blot
Product Name:
Anti-phospho-Histone H1 (Thr165)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Use Anti-phospho-Histone H1 (Thr165) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect phospho-Histone H1 (Thr165) also known as Histone H1.2, Histone H1d.

2°C – 8°C

BML-SE456-0050   Histone Deacetylase 1 (human) (recombinant)   品牌 Biomol

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Histone Deacetylase 1 (human) (recombinant)

货号： BML-SE456-0050      产品名称： Histone Deacetylase 1 (human) (recombinant)   品牌： Biomol 规格： 50 ug 3周到货 生化实验

HDAC1 (human), (recombinant) (His-tag)
Highly active
482 amino acids; HDAC1 from human cDNA, produced in a baculovirus expression system. Sequence is identical to that at Genbank accession #NM004964.
Product Specification
Alternative Name:Histone deacetylase 1
MW:55 kDa
Source:Produced in insect cells.
UniProt ID:Q13547
Formulation:Liquid. In 50mM TRIS, pH 8.0, 138mM sodium chloride and 10% glycerol.
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
Specific Activity:≥10 U/µg. One U=1 pmol/min at 37°C, 100µM, "Fluor de Lys™-SIRT1" Substrate (p53 K(Ac)-382 fluorogenic substrate (Prod. No. BML-KI177).
Long Term Storage:-80°C
Use/Stability:The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
Miscellaneous/General:Human HDAC1 (HD1) was the first protein to be linked to histone deacetylase activity. It is homologous to the yeast protein Rpd31, a relationship which has since come to define the “class I HDACs”. HDAC1 promotes transcriptional repression by deacetylating lysine ε-amino groups in histone N-terminal tails, a function frequently carried out in association with multi-protein transcription repression complexes such as NuRD3, Sin34 and CoREST6. Ubiquitously expressed in human tissues HDAC1-containing complexes appear to contribute the greater part of (at least class I) deacetylase activity in HeLa nuclear extracts. Aside from its interaction with co-repressors, HDAC1 activity may be regulated by post-translation modifications such as phosphorylation9 and sumoylation or binding to the inhibitor maspin, a tumor-suppressive serpin homolog. Although originally described as a “histone deacetylase”, HDAC1 has been shown to catalyze the regulatory deacetylation of non-histone proteins, including p53. Overexpression of HDAC1 has been found in various cancer types. HDAC inhibitors (HDACi) have shown considerable promise as anti-cancer agents and HDACi compounds from multiple chemical classes are in stages of drug development ranging from preclinical to phase III trials.

-80°C

07-677-I   Anti-acetyl-Histone H3 (Lys56)   品牌 Millipore

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Anti-acetyl-Histone H3 (Lys56)

货号： 07-677-I      产品名称： Anti-acetyl-Histone H3 (Lys56)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys56)
Recommended Replacement for: 07-677
Species Reactivity Key Applications Host Format Antibody Type
H  WB, DB, ChIP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys56) Antibody
Replaces:
07-677
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Histone H3 acetylated at Lys56.
Molecular Weight:
~17 kDa observed
Epitope:
Acetylated Lys56
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys56.
Background Information:
Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
View All »
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Dot Blot (Specificity) Analysis: A representative lot detected acetyl-Histone H3 (Lys56) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys56) from HeLa cells.
View All »
Control:
HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A.
Western Blot Analysis: A 1:405 dilution of this antibody detected acetyl-Histone H3 (Lys56) in sodium butyrate treated HeLa acid extract.
View All »
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P68431
Entrez Gene Number:
NP 003520
Gene Symbol:
HIST1H3A
H3FA
HIST1H3B
H3FL
HIST1H3C
H3FC
HIST1H3D
H3FB
HIST1H3E
H3FD
HIST1H3F
H3FI
HIST1H3G
H3FH
HIST1H3H
H3FK
HIST1H3I
H3FF
HIST1H3J
H3FJ
View All »
Alternate Names:
Histone H3.1
Histone H3/a
Histone H3/b
Histone H3/c
Histone H3/d
Histone H3/f
Histone H3/h
Histone H3/i
Histone H3/j
Histone H3/k
Histone H3/l
H3J56Ac
Histone H3 (acetyl K56)
Histone 3, H3
Histone cluster3, H3
Histone H3 K56ac
Histone H3 K56
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Dot Blot
Chromatin Immunoprecipitation (ChIP)
Product Name:
Anti-acetyl-Hist 2-8℃

14-1052   Human recombinant Histone H2A-H2B dimers   品牌 Millipore

发表于由

Human recombinant Histone H2A-H2B dimers

货号： 14-1052      产品名称： Human recombinant Histone H2A-H2B dimers   品牌： Millipore 规格： EA 三周到货 生化实验

Human recombinant histone H2A-H2B dimers
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PRODUCT FAMILY INFORMATION
Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
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Description:
Human recombinant histone H2A-H2B dimers
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Human recombinant histone H2A-H2B dimers expressed in E Coli. as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).
Background Information:
The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The nucleosome is the first order of DNA compaction in the nucleus, and is the basic unit of chromatin. The structure of chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of H2A, H2B, H3, and H4. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences, and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.
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Applications:
Human recombinant histone H2A-H2B dimers expressed in E Coli as fusion proteins with either an N-terminal FLAG or 6xHis epitopes & purified by Fast Protein Liquid Chromatography (FPLC).
Key Applications:
Activity Assay
Application Notes:
Suitable for in vitro chromatin assembly and enzyme activity assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Brand Family:
Upstate
Source:
Human
Presentation:
In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 2 M NaCl, 0.5 mM PMSF and 1 mM DTT
Storage Conditions:
Stable at -80ºC for 1 year from date of receipt.
Product Name:
Human recombinant histone H2A-H2B dimers
Concentration:
1 µg/µL
Host:
E. coli
Purity:
>98% purity by SDS PAGE

-80℃

14-1053   Human recombinant Histone H2AZ-H2B dimer   品牌 Millipore

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Human recombinant Histone H2AZ-H2B dimer

货号： 14-1053      产品名称： Human recombinant Histone H2AZ-H2B dimer   品牌： Millipore 规格： EA 三周到货 生化实验

Human recombinant histone H2AZ-H2B dimers
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PRODUCT FAMILY INFORMATION
Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
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Description:
Human recombinant histone H2AZ-H2B dimers
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Human recombinant histone H2AZ-H2B dimers expressed in E Coli. as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).
Background Information:
The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The H2AZ histone variant replaces conventional H2A in a subset of nucleosomes and plays an essential role in transcriptional regulation, autosilencing, gene silencing, and genome stability. The effects of histone variants and histone modifications on chromatin structure and gene expression can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin and essential tools, such as histone proteins and appropriate enzymes to assemble chromatin in vitro.
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Applications:
Human recombinant histone H2AZ-H2B dimers expressed in E Coli as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, & purified by Fast Protein Liquid Chromatography (FPLC)& verified by mass spectrometry.
Key Applications:
Activity Assay
Application Notes:
Suitable for in vitro chromatin assembly and enzyme activity assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Brand Family:
Upstate
Source:
Human
Presentation:
In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 2 M NaCl, 0.5 mM PMSF and 1 mM DTT
Storage Conditions:
Stable at -80ºC for 1 year from date of receipt.
Product Name:
Human recombinant histone H2AZ-H2B dimers
Concentration:
1 µg/µL
Host:
E. coli
Purity:
>98% purity by SDS PAGE

-80℃