标签归档：atr

PC538-50UL   ANTI-ATR (AB-2) RABBIT PAB   品牌 Merck

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ANTI-ATR (AB-2) RABBIT PAB

货号： PC538-50UL      产品名称： ANTI-ATR (AB-2) RABBIT PAB   品牌： Merck 规格： *** 三周 生化实验

Anti-ATR (Ab-2) (2122-2644) Rabbit pAb
Anti-Ataxia telangicctasia and Rad3 related protein
Recognizes the ~300 kDa ATR protein in human and mouse testis tissue extracts.
相关产品
PC538T  Anti-ATR (Ab-2) Rabbit pAb
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic peptide corresponding to amino acids 2122-2644 of human ataxia telangiectasia and Rad 3-related (ATR) protein
Form  Liquid
Formulation  Undiluted serum.
Species reactivity  hamster, human, mouse
Preservative  ≤0.1% sodium azide
Applications 
Immunoblotting (Western Blotting)
Avoid freeze/thaw  是的
Positive control  Human or mouse testis tissue
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Standard Handling

-20°C

189299-10MG   ATR/CDK Inhibitor, NU6027   品牌 Merck

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ATR/CDK Inhibitor, NU6027

货号： 189299-10MG      产品名称： ATR/CDK Inhibitor, NU6027   品牌： Merck 规格： *** 3周到货 生化实验

ATR/CDK Inhibitor, NU6027
A cell-permeable, pyrimidine derivative that acts as an ATP-competitive CDK Inhibitor (Ki = 2.5 µM for CDK1, and Ki = 1.3 µM for CDK2), and demonstrates cytostatic properties among a panel of 57 cancer cell lines. Also inhibits ATR kinase (IC50 = 6.7 µM in MCF7 cells), but does not interfere with irradiation-induced autophosphorylation of DNA-PK or ATM. Potentiates a range of DNA-damaging cytotoxic drugs such as hydroxyurea (1.8-fold) and cisplatin (1.4-fold) at 10 µM, but not the anti-mitotic paclitaxel. Attenuates G2/M arrest following DNA damage and inhibits RAD51 focus formation. Shown to be synthetically lethal when DNA single-strand break repair is impaired in PARP-inhibited cells.
产品信息
Form  Reddish-purple powder
Molar mass  251.28
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥98% by HPLC
Solubility  DMSO
Chemical formula  C11H17N5O2
CAS number  220036-08-8
商店和运输信息
Storage  -20°C
Ship  Shipped with Blue Ice or with Dry Ice
Standard Handling

-20°C

ABE389   Anti-phospho-ATR (Ser428)   品牌 Millipore

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Anti-phospho-ATR (Ser428)

货号： ABE389      产品名称： Anti-phospho-ATR (Ser428)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-ATR (Ser428)
Species Reactivity Key Applications Host Format Antibody Type
H, Mk  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-ATR (Ser428) Antibody
Promotional Text:
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Specificity:
This antibody recognizes ATR phosphorylated at (Ser428).
Molecular Weight:
~260 kDa observed
Epitope:
Phosphorylated (Ser428)
Immunogen:
KLH-conjugated linear peptide corresponding to human ATR phosphorylated at (Ser428).
Background Information:
The Ataxia-Telangiectasia and Rad3 related kinase (ATR) is a nuclear serine/threonine kinase that is part of the "first-response" system to DNA damage induced by a wide variety of factors including double-stranded breaks and replication stress. The localization of ATR to sites of DNA damage is aided by the Replication Protein A (RPA). Phospho – ATR (Ser428) targets a number of proteins including BRCA1, WRN, CHEK1, MCM2, and p53/TP53, and coordinates signaling pathways involved in DNA repair and apoptosis. ATR may also be involved in the phosphorylation of the histone H2A.X after replication stress, an early marker of DNA damage. However, ATR also mediates DNA replication and fork stability in normal cell cycles by activation of Chk1. Defective ATR contributes to Seckel syndrome type 1.
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Species Reactivity:
Human
Monkey
Species Reactivity Note:
Demonstrated to react in Human and Monkey. Other homologies: (63% mouse sequence homology).
Control:
UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates
Quality Assurance:
Evaluated by Western Blot in UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates.
Western Blot Analysis: A 1:200 dilution of this antibody detected ATR on 10 µg of UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13535
Entrez Gene Number:
NP_001175
Gene Symbol:
ATR
FRP1
Alternate Names:
Serine/threonine-protein kinase ATR
Ataxia telangiectasia and Rad3-related protein
FRAP-related protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
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UniProt Summary 2-8℃

ALX-210-798-R100   ATR (human), pAb   品牌 Alexis

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ATR (human), pAb

货号： ALX-210-798-R100      产品名称： ATR (human), pAb   品牌： Alexis 规格： 100.micro.l 三周 生化实验

ATR (human), pAb   
Product Specification
Alternative Name: Ataxia telangiectasia and Rad3-related protein
Host: Rabbit
Immunogen: Recombinant human ATR (ataxia telangiectasia and Rad3-related protein) (aa 400-460).
UniProt ID: Q13535
Application: Immunoprecipitation
                Western Blot (1:1’000)
Specificity: Recognizes human ATR. Detects a band of ~305kDa by Western blot.
Purity Detail: Affinity purified.
Formulation: Liquid. In PBS containing 0.05% sodium azide.
Handling: Avoid freeze/thaw cycles.
Long Term Storage: -20°C

-20°C

09-070   Anti-ATR, 1887-1902   品牌 Millipore

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Anti-ATR, 1887-1902

货号： 09-070      产品名称： Anti-ATR, 1887-1902   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-ATR
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ca, Eq, B  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-ATR Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes ATR.
Molecular Weight:
250 kDa
Immunogen:
KLH linear peptide corresponding to amino acids 1887-1902 of ATR.
Background Information:
ATR (Ataxia Telangiectasia and Rad3-related kinase) is a protein kinase that regulates cell cycle checkpoints and DNA repair. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1). ATR belongs to the PI3/PI4-kinase family, and is most closely related to ATM (Ataxia Telangiectasia Mutated kinase).
Species Reactivity:
Human
Mouse
Rat
Canine
Horse
Bovine
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Species Reactivity Note:
Proven to react with human, mouse, and rat. Predicted to react with dog, horse, and cow based on sequence homology.
Application Notes:
Western Blot (SNAP ID) Analysis: A 1:500 dilution from a previous lot of this antibody was used to was detect ATR in HeLa, NIH3T3, and C6 cell lysates.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot on HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect ATR in HeLa cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit serum in 0.1M Tris-Glycine (pH7.4) and 150mM NaCl containing 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C for 1 year from date of receipt.
UniProt Number:
Q13535
Entrez Gene Number:
NP_001175
Gene Symbol:
EC 2.7.11.1
FRP1
MEC1
SCKL
SCKL1
View All »
Alternate Names:
Ataxia telangiectasia and Rad3-related protein
FRAP-related protein 1
FRAP-related protein-1
MEC1, mitosis entry checkpoint 1, homolog
Rad3 related protein
ataxia telangiectasia and Rad3 related
ataxia telangiectasia and Rad3 related protein
protein kinase ATR
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
View All »
UniProt Summary:
FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate cons 2-8℃

3535-100   Fbx32/MAFbx/ATR-1 Antibody   品牌 Biovision

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Fbx32/MAFbx/ATR-1 Antibody

货号： 3535-100      产品名称： Fbx32/MAFbx/ATR-1 Antibody   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Fbx32/MAFbx/ATR-1 Antibody
Product Overview
Product Name: Fbx32/MAFbx/ATR-1 Antibody
Antibody Target: Fbx32
Alternative Name: F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide surrounding amino acid 56 of rat Fbx32.
Accession#: Q91Z62
Gene ID: 171043
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Fbx32 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot
Application & Usage: Western Blot analysis (1-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3535BP-50) is available separately.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Atrogin-1 (Atr1) is also known as Muscle Atrophy F-box (MAFbx). It is an E3 ubiquitin ligase that initiates ATPdependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx is approximately a 43kDa protein.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

3535BP-50   Fbx32/MAFbx/ATR-1 Blocking Peptide   品牌 Biovision

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Fbx32/MAFbx/ATR-1 Blocking Peptide

货号： 3535BP-50      产品名称： Fbx32/MAFbx/ATR-1 Blocking Peptide   品牌： Biovision 规格： 50 μg 3周到货 生化实验

Fbx32/MAFbx/ATR-1 Blocking Peptide
Product Overview
Antibody Target: MyD88
Accession #: Q6Y1S1
Gene ID: 301059
Appearance: Colorless liquid
Concentration: 0.5mg/ml
Formulation: 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Temp.: -20°C
Shipping: Gel Pack
Application & Usage: The peptide is used for blocking the antibody activity of MyD88 (Cat.# 3244R-100). It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Description: 50 ug blocking the antibody activity of MyD88 (Cat.# 3244R-100).
Handling: The peptide solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C