标签归档：rep

rep-lps   HEK-Blue LPS Detection Kit   品牌 Invivogen

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HEK-Blue LPS Detection Kit

货号： rep-lps      产品名称： HEK-Blue LPS Detection Kit   品牌： Invivogen 规格： 1 kit 二周到货 生化试验

HEK-Blue™ LPS Detection Kit
Cell-Based Assay for the Detection of Endotoxins in Biological Samples
The HEK-Blue™ LPS Detection Kit is based on the ability of TLR4 to recognize structurally different LPS from gram-negative bacteria and in particular lipid A, their toxic moiety. Proprietary cells engineered to become extremely sensitive to LPS, called HEK-Blue™-4 cells, are the main feature of this endotoxin detection kit. The presence of very low concentrations of LPS, starting as low as 0.03 ng/ml, are detected by the HEK-Blue™-4 cells leading to the activation of NF-κB. Using HEK-Blue™ Detection, a specific detection medium, NF-κB activation can be observed with the naked eye or quantified by reading the OD at 650 nm.
Requires only basic cell culture knowledge
Hands-on time: < 1 hour. Gives results after overnight incubation
Detects only endotoxins – No false positive
Up to 500 samples can be tested with the kit. To perform further assays, only the reagents need to be reordered
Detection range: 1.5 EU/ml in the sample

rep-lz-c   LacZ Cell Staining Kit   品牌 Invivogen

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LacZ Cell Staining Kit

货号： rep-lz-c      产品名称： LacZ Cell Staining Kit   品牌： Invivogen 规格： 1 kit (100 reactions) 二周到货 生化试验

LacZ Cell Staining Kit
LacZ Cell Staining Kit allows you to determine the percentage of transfected cells expressing the lacZ gene. All reagents are provided to prepare the fixative, staining and rinse solutions. The assay can be completed in 30 minutes, and the blue precipitate can take from 30 minutes to overnight to appear. The LacZ Cell Staining Kit contains sufficient reagent to stain one hundred 35 mm plates.
Short hands on time: 30 minutes
Shipped at room temperature
All components should be stored at -20°C except PBS at 4°C

rep-lz-t   LacZ Tissue Staining Kit   品牌 Invivogen

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LacZ Tissue Staining Kit

货号： rep-lz-t      产品名称： LacZ Tissue Staining Kit   品牌： Invivogen 规格： 1 kit (100 ml) 二周到货 生化试验

Tissue Staining Kit
LacZ Tissue Staining Kit allows the detection of transduced cells expressing the lacZ gene within fresh or frozen tissues. All reagents are provided to prepare the buffer, fixative and staining solutions. The staining of the tissues can be observed in 5 to 24 hours. The LacZ Tissue Staining Kit contains sufficient reagent to prepare 100 ml solutions.
LacZ Tissue Staining Kit A staining kit to determine β-Galactosidase activity in intact tissues

rep-pt2   PlasmoTest   品牌 Invivogen

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PlasmoTest

货号： rep-pt2      产品名称： PlasmoTest   品牌： Invivogen 规格： 1 kit (500 samples) 二周到货 生化试验

PlasmoTest™
PlasmoTest™ provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. This assay is the first to utilize cells to signal the presence of mycoplasma.
PlasmoTest™ features two major constituents: the Mycoplasma sensor cells and the HEK-Blue™ Detection medium.
The Mycoplasma sensor cells detect the presence of mycoplasmas leading to a color change of the HEK-Blue™ Detection medium. The Mycoplasma sensor cells recognize mycoplasmas through Toll-like Receptor 2 (TLR2), a pathogen recognition receptor. In the presence of mycoplasmas, TLR2 initiates a signaling cascade leading to the activation of NF-kB and other transcription factors. These transcription factors induce the secretion of SEAP (secreted embryonic alkaline phosphatase) in the supernatant which is readily detected by the purple/blue coloration of the HEK-Blue™ Detection medium.
• Requires only basic cell culture knowledge
• Hands-on time less than 1 hour. Gives results after overnight incubation
• Detects all cell culture contaminants
• Detects 5.102-5.105 cfu/ml mycoplasmas
• No false positive: a positive result indicates the presence of a cell culture contaminant
• Available in two quantities :
– Kit rep-pt1 : test up to 250 samples
– Kit rep-pt2 : test up to 500 samples
To perform further assays, only the reagents need to be reordered.

rep-ptrk   PlasmoTest Reagent Kit   品牌 Invivogen

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PlasmoTest Reagent Kit

货号： rep-ptrk      产品名称： PlasmoTest Reagent Kit   品牌： Invivogen 规格： 500 samples 二周到货 生化试验

PlasmoTest™ Reagent Kit
PlasmoTest™  provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. Buy the PlasmoTest™ kit once then reorder only the reagents to perform further assays. PlasmoTest™ Reagent Kit allows to test 500 samples.
Shipped at room temperature

rep-qb1   QUANTI-Blue   品牌 Invivogen

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QUANTI-Blue

货号： rep-qb1      产品名称： QUANTI-Blue   品牌： Invivogen 规格： 5 pouches (5 x 100 ml) 三周到货 生化试验

QUANTI-Blue™
QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase activity (AP) in a biological sample, such as supernatants of cell cultures. In particular, QUANTI-Blue™ provides an easy and rapid mean to detect and quantify secreted embryonic alkaline phosphatase (SEAP), a reporter widely used for in vitro and in vivo analytical studies.
In the presence of alkaline phosphatase, the color of QUANTI-Blue™ changes from pink to purple/blue. The intensity of the blue hue reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.
QUANTI-Blue™ is useful to monitor activation in reporter cell lines.
Short hands-on-time: < 10 min
Easy to prepare: just add water
Highly sensitive
High saturation threshold
Allows high-throughput screening

rep-qb2   QUANTI-Blue   品牌 Invivogen

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QUANTI-Blue

货号： rep-qb2      产品名称： QUANTI-Blue   品牌： Invivogen 规格： 10 pouches (10 x 100 ml) 三周到货 生化试验

QUANTI-Blue™
QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase activity (AP) in a biological sample, such as supernatants of cell cultures. In particular, QUANTI-Blue™ provides an easy and rapid mean to detect and quantify secreted embryonic alkaline phosphatase (SEAP), a reporter widely used for in vitro and in vivo analytical studies.
In the presence of alkaline phosphatase, the color of QUANTI-Blue™ changes from pink to purple/blue. The intensity of the blue hue reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.
QUANTI-Blue™ is useful to monitor activation in reporter cell lines.
Short hands-on-time: < 10 min
Easy to prepare: just add water
Highly sensitive
High saturation threshold
Allows high-throughput screening

rep-sap   SEAP Reporter Assay Kit   品牌 Invivogen

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SEAP Reporter Assay Kit

货号： rep-sap      产品名称： SEAP Reporter Assay Kit   品牌： Invivogen 规格： 1 kit 三周到货 生化试验

SEAP Reporter Assay Kit
The SEAP Reporter Assay Kit provides a rapid and convenient method to determine the levels of SEAP in the culture medium of transfected cells expressing the seap gene.
SEAP catalyzes the hydrolysis of p-Nitrophenyl phosphate producing a yellow end product that can be read spectrophotometrically at 405 nm.
Short hands-on-time:  less than 1 hour
Obtention of results: 10 to 40 minutes
Sensitivity: as little as 10-10 g/ml of SEAP
Concentration range: 1 ng – 1 µg/ml

12935145   STEALTH RNAI GFP REP CTRL 20UM   品牌 Invitrogen

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STEALTH RNAI GFP REP CTRL 20UM

货号： 12935145      产品名称： STEALTH RNAI GFP REP CTRL 20UM   品牌： Invitrogen 规格： 1 X 250UL 三周到货 生化试验

Stealth RNAi™ siRNA GFP Reporter Control
Stealth RNAi™ siRNA Reporter Controls are ideal for use in RNA interference (RNAi) experiments to help you optimize your transfection conditions in any vertebrate cell line. These controls are: –Designed to efficiently knock-down its intended target –Not homologous to anything in vertebrate transcriptome
RNAi Type: siRNA 
Target Gene: GFP 
Control Type: Gene Knockdown Positive Control 
Label or Dye: Unconjugated 
Product Size: 250 µL
Shipping Condition: Dry Ice 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
This individual control duplex is supplied as 250 µl at 20 µM and includes 1 ml of 1X RNA Annealing/Dilution Buffer. Store at -20°C. Products are guaranteed stable for 6 months when properly stored.

12935146   STEALTH RNAI LUC REP CTRL 20UM   品牌 Invitrogen

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STEALTH RNAI LUC REP CTRL 20UM

货号： 12935146      产品名称： STEALTH RNAI LUC REP CTRL 20UM   品牌： Invitrogen 规格： 1 X 250UL 三周到货 生化试验

Stealth RNAi™ siRNA Luciferase Reporter Control
Stealth RNAi™ siRNA Reporter Controls are ideal for use in RNA interference (RNAi) experiments to help you optimize your transfection conditions in any vertebrate cell line. These controls are: –Designed to efficiently knock-down its intended target –Not homologous to anything in vertebrate transcriptome
RNAi Type: siRNA 
Target Gene: Luciferase 
Control Type: Gene Knockdown Positive Control
Label or Dye: Unconjugated 
Product Size: 250 µL 
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
This individual control duplex is supplied as 250 µl at 20 µM and includes 1 ml of 1X RNA Annealing/Dilution Buffer. Store at -20°C. Products are guaranteed stable for 6 months when properly stored.

12935148   STEALTH RNA BLAC REP CTRL 20UM   品牌 Invitrogen

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STEALTH RNA BLAC REP CTRL 20UM

货号： 12935148      产品名称： STEALTH RNA BLAC REP CTRL 20UM   品牌： Invitrogen 规格： 1 X 250UL 三周到货 生化试验

Stealth RNAi™ siRNA β-Lactamase Reporter Control
Stealth RNAi™ siRNA Reporter Controls are ideal for use in RNA interference (RNAi) experiments to help you optimize your transfection conditions in any vertebrate cell line. These controls are: –Designed to efficiently knock-down its intended target –Not homologous to anything in vertebrate transcriptome
RNAi Type: siRNA 
Target Gene: β Lactamase
Control Type: Gene Knockdown Positive Control
Label or Dye: Unconjugated 
Product Size: 250 µL 
Shipping Condition: Dry Ice 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
This individual control duplex is supplied as 250 µl at 20 µM and includes 1 ml of 1X RNA Annealing/Dilution Buffer. Store at -20°C. Products are guaranteed stable for 6 months when properly stored.

 

rep-pt1   PlasmoTest   品牌 Invivogen

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PlasmoTest

货号： rep-pt1      产品名称： PlasmoTest   品牌： Invivogen 规格： 1 kit (250 samples) 二周到货 生化试验

PlasmoTest™
PlasmoTest™ provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. This assay is the first to utilize cells to signal the presence of mycoplasma.
PlasmoTest™ features two major constituents: the Mycoplasma sensor cells and the HEK-Blue™ Detection medium.
The Mycoplasma sensor cells detect the presence of mycoplasmas leading to a color change of the HEK-Blue™ Detection medium. The Mycoplasma sensor cells recognize mycoplasmas through Toll-like Receptor 2 (TLR2), a pathogen recognition receptor. In the presence of mycoplasmas, TLR2 initiates a signaling cascade leading to the activation of NF-kB and other transcription factors. These transcription factors induce the secretion of SEAP (secreted embryonic alkaline phosphatase) in the supernatant which is readily detected by the purple/blue coloration of the HEK-Blue™ Detection medium.
• Requires only basic cell culture knowledge
• Hands-on time less than 1 hour. Gives results after overnight incubation
• Detects all cell culture contaminants
•Detects 5.102-5.105 cfu/ml mycoplasmas
• No false positive: a positive result indicates the presence of a cell culture contaminant
• Available in two quantities :
– Kit rep-pt1 : test up to 250 samples
– Kit rep-pt2 : test up to 500 samples
To perform further assays, only the reagents need to be reordered.

rep-qlc1   Quanti-Luc   品牌 Invivogen

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Quanti-Luc

货号： rep-qlc1      产品名称： Quanti-Luc   品牌： Invivogen 规格： 2 pouches (2 x 25ml) 三周到货 生化试验

QUANTI-Luc™
A coelenterazine-based luminescence assay reagent
QUANTI-Luc™, is an assay reagent containing all the components required to quantitively measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases.
QUANTI-Luc™ is optimized for use with Lucia luciferase reporter cell lines for fast and efficient real-time measurements directly from the cell culture media. QUANTI-Luc™ contains the coelenterazine substrate for the luciferase reaction, which produces a light signal that is quantified using a luminometer and expressed as relative light units (RLU).
The signal produced correlates to the amount of luciferase protein expressed, indicating promoter activity in the reporter assay.
Ready to use – Just add water
Cost effective – One pouch prepares 5 x 96 well plates
Practical -Working reagent stable for at least one month
Use with Lucia luciferase:
Exceptional sensitivity and reproducibility
No cell lysis required
Rapid acquisition of results

rep-qlc2   Quanti-Luc   品牌 Invivogen

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Quanti-Luc

货号： rep-qlc2      产品名称： Quanti-Luc   品牌： Invivogen 规格： 5 pouches (5 x 25ml) 三周到货 生化试验

QUANTI-Luc™
A coelenterazine-based luminescence assay reagent
QUANTI-Luc™, is an assay reagent containing all the components required to quantitively measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases.
QUANTI-Luc™ is optimized for use with Lucia luciferase reporter cell lines for fast and efficient real-time measurements directly from the cell culture media. QUANTI-Luc™ contains the coelenterazine substrate for the luciferase reaction, which produces a light signal that is quantified using a luminometer and expressed as relative light units (RLU).
The signal produced correlates to the amount of luciferase protein expressed, indicating promoter activity in the reporter assay.
Ready to use – Just add water
Cost effective – One pouch prepares 5 x 96 well plates
Practical -Working reagent stable for at least one month
Use with Lucia luciferase:
Exceptional sensitivity and reproducibility
No cell lysis required
Rapid acquisition of results