标签归档：at

pbroad2   pBROAD2-mcs   品牌 Invivogen

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pBROAD2-mcs

货号： pbroad2      产品名称： pBROAD2-mcs   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pBROAD2
pBROAD2 plasmids were designed for the expression of your favorite gene in virtually all tissues of new transgenic mice and rats. pBROAD2 plasmids feature the ubiquitous human ROSA26 promoter, a high CpG content and TATA-less promoter. It directs in vivo expression of the transgene in most tissue types including cells of the developing germ line and hematopoietic lineage. It is also very effective in vitro in a very broad range of mammalian cell lines.
– Strong and Ubiquitous Promoter
– Features Multiple Cloning Site or Synthetic LacZ Reporter Gene
– Easy Excision of the E. coli Region
– Efficient in vivo Arrest of the Transgene Transcription

pfc1-hg1e9   pFUSE-hIgG1e9-Fc1   品牌 Invivogen

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pFUSE-hIgG1e9-Fc1

货号： pfc1-hg1e9      产品名称： pFUSE-hIgG1e9-Fc1   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-hIgG1e9-Fc
pFUSE-hIgG1e9-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated S239D/I332E/G236A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-hIgG1e9-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype: IgG1 human
Mutations: S239D/I332E/G236A
Characteristics:
– Increased FcγRIIa/FcγRIIb ratio
– Increased macrophage phagocytosis
Both plasmids are selectable with Zeocin™.

pfc1-hg2e1   pFUSE-hIgG2e1-Fc1   品牌 Invivogen

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pFUSE-hIgG2e1-Fc1

货号： pfc1-hg2e1      产品名称： pFUSE-hIgG2e1-Fc1   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-hIgG2e1-Fc
pFUSE-hIgG2e1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG2 heavy chain mutated K322A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-hIgG2e1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype : IgG2 human
Mutations: K322A
Characteristics:
– Reduced binding to C1q
– Reduced CDC
Both plasmids are selectable with Zeocin™.

pfc1-mgin2   pINFUSE-mIgG2B-Fc1   品牌 Invivogen

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pINFUSE-mIgG2B-Fc1

货号： pfc1-mgin2      产品名称： pINFUSE-mIgG2B-Fc1   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pINFUSE-mIgG2b-Fc
pINFUSE-mIgG2b-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG2b heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. A short intron is present between each region (one intron between the hinge and CH2 and one intron between CH2 and CH3). The presence of introns is known to enhance the level of gene expression as splicing is known to promote rapid and efficient mRNA export.
pINFUSE-mIgG2b-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted
Murine IgG2b dispays high ADCC and CDC.
Both plasmids are selectable with Zeocin™.

pfc2-hg1e1   pFUSE-hIgG1e1-Fc2   品牌 Invivogen

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pFUSE-hIgG1e1-Fc2

货号： pfc2-hg1e1      产品名称： pFUSE-hIgG1e1-Fc2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-hIgG1e1-Fc
pFUSE-hIgG1e1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated T250Q/M428L and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-hIgG1e1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype: IgG1 human
Mutations: T250Q/M428L
Characteristics:
– Increased binding to neonatal FcR (FcRn)
– Increased half-life
Both plasmids are selectable with Zeocin™.

pfc2-hg1e2   pFUSE-hIgG1e2-Fc2   品牌 Invivogen

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pFUSE-hIgG1e2-Fc2

货号： pfc2-hg1e2      产品名称： pFUSE-hIgG1e2-Fc2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-hIgG1e2-Fc
pFUSE-hIgG1e2-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. The engineered hIgG1e2 Fc contains the following mutations: M252Y/S254T/T256E and H433K/N434F.
pFUSE-hIgG1e2-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype: IgG1 human
Mutations: M252Y/S254T/T256E + H433K/N434F
Characteristics:
– Increased binding to neonatal FcR (FcRn)
– Increased half-life
Both plasmids are selectable with Zeocin™.

pfc2-hg1e9   pFUSE-hIgG1e9-Fc2   品牌 Invivogen

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pFUSE-hIgG1e9-Fc2

货号： pfc2-hg1e9      产品名称： pFUSE-hIgG1e9-Fc2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-hIgG1e9-Fc
pFUSE-hIgG1e9-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated S239D/I332E/G236A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-hIgG1e9-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype: IgG1 human
Mutations: S239D/I332E/G236A
Characteristics:
– Increased FcγRIIa/FcγRIIb ratio
– Increased macrophage phagocytosis
Both plasmids are selectable with Zeocin™.

pfc2-hgin1   pINFUSE-hIgG1-Fc2   品牌 Invivogen

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pINFUSE-hIgG1-Fc2

货号： pfc2-hgin1      产品名称： pINFUSE-hIgG1-Fc2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pINFUSE-hIgG1-Fc
pINFUSE-hIgG1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. A short intron is present between each region (one intron between the hinge and CH2 and one intron between CH2 and CH3). The presence of introns is known to enhance the level of gene expression as splicing is known to promote rapid and efficient mRNA export.
pINFUSE-hIgG1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted
Human IgG1 displays high ADCC and CDC, and is the most suitable for therapeutic use against pathogens and cancer cells.
Both plasmids are selectable with Zeocin™.

pfuse-hchg1e2   pFUSE-CHIg-hG1e2   品牌 Invivogen

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pFUSE-CHIg-hG1e2

货号： pfuse-hchg1e2      产品名称： pFUSE-CHIg-hG1e2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e2
pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations
• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence
Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
– Increased binding to FcRn
– Increased half-life
Both plasmids are selectable with Zeocin™.

pfuse-rtg2bfc1   pFUSE-rtIgG2B-Fc1   品牌 Invivogen

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pFUSE-rtIgG2B-Fc1

货号： pfuse-rtg2bfc1      产品名称： pFUSE-rtIgG2B-Fc1   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-rtIgG2b-Fc (rat)
pFUSE-rtIgG2b-Fc1 is a cloning plasmid for the generation ofa Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the rat IgG2b heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-rtIgG2b-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype : IgG2b rat
Effector Activities : ADCC++, CDC ++
Protein A binding: –
Protein G binding: ++
Protein L binding: ++++
Both plasmids are selectable with Zeocin™.

pfuse-rtg2bfc2   pFUSE-rtIgG2B-Fc2   品牌 Invivogen

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pFUSE-rtIgG2B-Fc2

货号： pfuse-rtg2bfc2      产品名称： pFUSE-rtIgG2B-Fc2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-rtIgG2b-Fc (rat)
pFUSE-rtIgG2b-Fc1 is a cloning plasmid for the generation ofa Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the rat IgG2b heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-rtIgG2b-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Isotype : IgG2b rat
Effector Activities : ADCC++, CDC ++
Protein A binding: –
Protein G binding: ++
Protein L binding: ++++
Both plasmids are selectable with Zeocin™.

pfusess-hchg1e2   pFUSEss-CHIg-hG1e2   品牌 Invivogen

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pFUSEss-CHIg-hG1e2

货号： pfusess-hchg1e2      产品名称： pFUSEss-CHIg-hG1e2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e2
pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations
• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence
Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
– Increased binding to FcRn
– Increased half-life
Both plasmids are selectable with Zeocin™.

BML-SA608-0050   AT1 receptor (extracellular), pAb   品牌 Biomol

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AT1 receptor (extracellular), pAb

货号： BML-SA608-0050      产品名称： AT1 receptor (extracellular), pAb   品牌： Biomol 规格： 50 ul 3周到货 生化实验

AT1 receptor (extracellular), pAb
Product Specification
Alternative Name:Angiotensin II receptor type 1
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 4-18 of human AT1.
UniProt ID:P30556
Application:Flow Cytometry
Immunocytochemistry
Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human, mouse and rat AT1.
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
Reconstitution:Reconstitute antibody with either 50µl or 200µl of distilled water depending upon the package size purchased.
Reconstitute control peptide with 100µl distilled water.
Long Term Storage:-20°C

-20°C

BML-SA609-0050   AT2 receptor (extracellular), pAb   品牌 Biomol

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AT2 receptor (extracellular), pAb

货号： BML-SA609-0050      产品名称： AT2 receptor (extracellular), pAb   品牌： Biomol 规格： 50 ul 3周到货 生化实验

AT2 receptor (extracellular), pAb
Angiotensin II receptor type 2 or AT2 is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Research indicates that the AT2 receptor signals through the Gia2 and the Gia3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. The AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain.
Product Specification
Alternative Name:Angiotensin II receptor type 2
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 21-35 of rat AT2.
UniProt ID:P35351
Application:Western Blot
Specificity:Recognizes mouse and rat AT2 receptor.
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
Long Term Storage:-20°C

-20°C

BML-SA609-0200   AT2 receptor (extracellular), pAb   品牌 Biomol

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AT2 receptor (extracellular), pAb

货号： BML-SA609-0200      产品名称： AT2 receptor (extracellular), pAb   品牌： Biomol 规格： 200 ul 3周到货 生化实验

AT2 receptor (extracellular), pAb
Angiotensin II receptor type 2 or AT2 is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Research indicates that the AT2 receptor signals through the Gia2 and the Gia3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. The AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain.
Product Specification
Alternative Name:Angiotensin II receptor type 2
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 21-35 of rat AT2.
UniProt ID:P35351
Application:Western Blot
Specificity:Recognizes mouse and rat AT2 receptor.
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
Long Term Storage:-20°C

-20°C