标签归档：ant

pfuse-hchg1e2   pFUSE-CHIg-hG1e2   品牌 Invivogen

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pFUSE-CHIg-hG1e2

货号： pfuse-hchg1e2      产品名称： pFUSE-CHIg-hG1e2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e2
pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations
• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence
Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
– Increased binding to FcRn
– Increased half-life
Both plasmids are selectable with Zeocin™.

pfuse-hchg1e7   pFUSE-CHIg-hG1e7   品牌 Invivogen

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pFUSE-CHIg-hG1e7

货号： pfuse-hchg1e7      产品名称： pFUSE-CHIg-hG1e7   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e7
pFUSE-CHIg-hG1e7 is a cloning plasmid that expresses the human IgG1 heavy chain constant region mutated K326W / E333S. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e7 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain mutated K326W / E333S
• pFUSEss-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain mutated K326W / E333S and the IL2 signal sequence
Characteristics of K326W / E333S mutations:
– Increased binding to C1q
– Increased CDC
Both plasmids are selectable with Zeocin™.

pfuse-hg1sp   pFUSE-SEAP-hG1-Fc   品牌 Invivogen

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pFUSE-SEAP-hG1-Fc

货号： pfuse-hg1sp      产品名称： pFUSE-SEAP-hG1-Fc   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-SEAP-hG1-Fc
pFUSE-SEAP-hIgG1-Fc allows the production of SEAP-human IgG1-Fc fusion proteins. This plasmid can be used to make recombinant SEAP-Fc fusion proteins or used as a transfection control in experiments with other pFUSE-Fc constructs.
Quantification of SEAP-Fc expression can be determined using SEAP Reporter Assay Kit or QUANTI-Blue™.
Isotype: Human IgG1
This plasmid is selectable with Zeocin™.

pfuse-hg3sp   pFUSE-SEAP-hG3-Fc   品牌 Invivogen

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pFUSE-SEAP-hG3-Fc

货号： pfuse-hg3sp      产品名称： pFUSE-SEAP-hG3-Fc   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-SEAP-hG3-Fc
pFUSE-SEAP-hIgG3-Fc allows the production of SEAP-human IgG3-Fc fusion proteins. This plasmid can be used to make recombinant SEAP-Fc fusion proteins or used as a transfection control in experiments with other pFUSE-Fc constructs.
Quantification of SEAP-Fc expression can be determined using SEAP Reporter Assay Kit or QUANTI-Blue™.
Isotype: Human IgG3
This plasmid is selectable with Zeocin™.

pfuse-rtsp   pFUSE-SEAP-rtFc   品牌 Invivogen

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pFUSE-SEAP-rtFc

货号： pfuse-rtsp      产品名称： pFUSE-SEAP-rtFc   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-SEAP-rtFc
pFUSE-SEAP-rtIgG-Fc allows the production of a SEAP-Fc fusion protein with the Fc region of the rat IgG2b. This plasmid can be used to make recombinant SEAP-Fc fusion proteins or used as a transfection control in experiments with other pFUSE-Fc constructs.
Quantification of SEAP-Fc expression can be determined using SEAP Reporter Assay Kit or QUANTI-Blue™.
Isotype: Rat IgG2b
This plasmid is selectable with Zeocin™.

pfusess-hchg1e2   pFUSEss-CHIg-hG1e2   品牌 Invivogen

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pFUSEss-CHIg-hG1e2

货号： pfusess-hchg1e2      产品名称： pFUSEss-CHIg-hG1e2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e2
pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations
• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence
Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
– Increased binding to FcRn
– Increased half-life
Both plasmids are selectable with Zeocin™.

pfusess-hchg1e7   pFUSEss-CHIg-hG1e7   品牌 Invivogen

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pFUSEss-CHIg-hG1e7

货号： pfusess-hchg1e7      产品名称： pFUSEss-CHIg-hG1e7   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e7
pFUSE-CHIg-hG1e7 is a cloning plasmid that expresses the human IgG1 heavy chain constant region mutated K326W / E333S. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e7 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain mutated K326W / E333S
• pFUSEss-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain mutated K326W / E333S and the IL2 signal sequence
Characteristics of K326W / E333S mutations:
– Increased binding to C1q
– Increased CDC
Both plasmids are selectable with Zeocin™.

PC522-100UG   ANTI-ANT-1 (AB-1) RABBIT PAB   品牌 Merck

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ANTI-ANT-1 (AB-1) RABBIT PAB

货号： PC522-100UG      产品名称： ANTI-ANT-1 (AB-1) RABBIT PAB   品牌： Merck 规格： *** 3周到货 生化实验

Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb
Recognizes the ~35 kDa ANT-1 protein in doxorubicin-treated MCF-7 cells or breast carcinoma tissue.
Antibody Target Gene Symbol: ANK3
Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
Hu Entrez ID: 288 (Related Antibodies: NB20, AP1034, PC523, AP1057)
Mu Entrez ID: 11735
Rat Entrez ID: 361833
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic peptide corresponding to amino acids 2-11 of human ANT-1
Form  Liquid
Formulation  In 50 mM sodium phosphate buffer, 50% glycerol.
Species reactivity  human
Preservative  None
Applications 
Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Avoid freeze/thaw  是的
Positive control  doxorubicin-treated MCF7 cells, or breast carcinoma tissue
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Standard Handling

-20°C

ant-fgl-5   FITC-Geldanamycin   品牌 Invivogen

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FITC-Geldanamycin

货号： ant-fgl-5      产品名称： FITC-Geldanamycin   品牌： Invivogen 规格： 5 mg 二周到货 生化试验

FITC-Geldanamycin
FITC-labeled geldanamycin
FITC-geldanamycin is a fluorescent derivative of geldanamycin. The fluorescein-5-isothiocyanate dye was linked to geldanamycin at the C17 position [1].
FITC-geldanamycin binds tightly to Hsp90. This interaction can be competed by Hsp90 inhibitors such as 17-AAG. Therefore, FITC-geldanamycin can be used to screen for new Hsp90 inhibitors by measuring its binding to Hsp90 through a fluorescent polarization assay.
Formula: C67H85N7O15S
Molecular weight: 1260.5
Solubility: DMSO (10 mg/ml)
Working concentration: 1 nM – 10 μM

ant-fgl-1   FITC-Geldanamycin   品牌 Invivogen

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FITC-Geldanamycin

货号： ant-fgl-1      产品名称： FITC-Geldanamycin   品牌： Invivogen 规格： 1 mg 二周到货 生化试验

FITC-Geldanamycin
FITC-labeled geldanamycin
FITC-geldanamycin is a fluorescent derivative of geldanamycin. The fluorescein-5-isothiocyanate dye was linked to geldanamycin at the C17 position [1].
FITC-geldanamycin binds tightly to Hsp90. This interaction can be competed by Hsp90 inhibitors such as 17-AAG. Therefore, FITC-geldanamycin can be used to screen for new Hsp90 inhibitors by measuring its binding to Hsp90 through a fluorescent polarization assay.
Formula: C67H85N7O15S
Molecular weight: 1260.5
Solubility: DMSO (10 mg/ml)
Working concentration: 1 nM – 10 μM

05-983   Ant-PKC (a, b, g), ms monoclonal   品牌 Millipore

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Ant-PKC (a, b, g), ms monoclonal

货号： 05-983      产品名称： Ant-PKC (a, b, g), ms monoclonal   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PKC Antibody (α, β, γ), clone M110
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  ELISA, IP, WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-PKC Antibody (α, β, γ), clone M110
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes PKC α, β, γ. Does not cross react with other PKC isoforms.
Molecular Weight:
80-82 kDa
Immunogen:
Recombinant protein of human PKCγ amino acids 499-697.
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Protein A purfied
Presentation:
100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
P17252
P05771
P05129
Entrez Gene Number:
NP_002728, NP_002730
Gene Symbol:
PRKCA
PKC-A
AAG6
PKC-alpha
MGC129901
MGC129900
PRKACA
PKCA
Alternate Names:
PRKCA, PRKCB, PRKCG, protein kinase C, alpha, beta, gamma
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
ELISA
Immunoprecipitation
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
UniProt Summary:
FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 672 amino acids; 76764 Da
SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & -20°C

07-167   ANT-BLYS/TAL-1/BAF PC 12-303   品牌 Millipore

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ANT-BLYS/TAL-1/BAF PC 12-303

货号： 07-167      产品名称： ANT-BLYS/TAL-1/BAF PC 12-303   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Blys/TALL-1/BAFF/THANK Antibody
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REFERENCES
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Weifeng Xu, Bing He, April Chiu, Amy Chadburn, Meimei Shan, Malwina Buldys, Aihao Ding, Daniel M Knowles, Paul A Santini, Andrea Cerutti (2007) Nature immunology.8:294-303
Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
Patrizia Scapini, Antonio Carletto, Bernardetta Nardelli, Federica Calzetti, Viktor Roschke, Flavia Merigo, Nicola Tamassia, Sara Pieropan, Domenico Biasi, Andrea Sbarbati, Silvano Sozzani, Lisa Bambara, Marco A Cassatella (2005) Blood.105:830-7
Macrophage- and dendritic cell–dependent regulation of human B-cell proliferation requires the TNF family ligand BAFF.
Andrew Craxton, Dario Magaletti, Elizabeth J Ryan, Edward A Clark, Andrew Craxton, Dario Magaletti, Elizabeth J Ryan, Edward A Clark (2003) Blood.101:4464-71
TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease.
Gross, J A, et al. (2000) Nature, 404: 995-9 (2000)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-Blys/TALL-1/BAFF/THANK Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Blys/TALL-1/BAFF/THANK
Molecular Weight:
32kDa
Immunogen:
peptide corresponding to amino acids 254-269 of human Blys (EEGDELQLAIPRENAQ)
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells or HeLa nuclear extract
Purification Method:
Protein G chromatography
Presentation:
PBS containing 0.02% sodium azide
Storage Conditions:
2 years at -20°C
UniProt Number:
Q9Y275
Entrez Gene Number:
NM_006573
Gene Symbol:
TNFSF13B
DTL
TALL-1
ZTNF4
BLyS
THANK
TNFSF20
CD257
TALL1
BAFF
BLYS
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q9Y275 # Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
SIZE: 285 amino acids; 31223 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & -20℃