Anti-RAS-Related protein Ral-A

货号： ABS223      产品名称： Anti-RAS-Related protein Ral-A   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-RAS-Related protein Ral-A
Recommended Replacement for: 07-2123
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-RAS-Related protein Ral-A Antibody
Replaces:
07-2123
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Molecular Weight:
~26 kDa observed
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of human RAS-Related protein Ral-A.
Background Information:
RalA is a Ras-family GTPase which has a well-documented role in tumorigenesis. RalA is activated by Ras GTPases and in turn interacts with a number of effector molecules, most notably the exocyst or Sec6/8 complex . The Sec6/8 complex is an octameric structure which plays a key role in exocytosis of secretory vesicles by forming a bridge between vesicular and plasma membranes. RalA indirectly mediates this process as it is involved in the assembly of Sec6/8; RalA interacts with components of the Sec6/8 complex including Exo84 and Sec5. RalA may promote tumorigenesis through a Ras-induced pathway involved in cell transformation, actin dynamics or exocytosis.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react in human. Predicted to react with Rat, Mouse, Equine, Porcine, and Bovine based on 100% sequence homology.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected RAS-Related protein Ral-A on 10 µg of HeLa cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P11233
Entrez Gene Number:
NP_005393
Gene Symbol:
RALA
RAL
Alternate Names:
Ras-related protein Ral-A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008].
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UniProt Summary:
FUNCTION: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
2-8℃