标签归档：eks

ADI-EKS-700B   HSP70 ELISA kit   品牌 Enzo

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HSP70 ELISA kit

货号： ADI-EKS-700B      产品名称： HSP70 ELISA kit   品牌： Enzo 规格： 1 x 96 wells 3周到货 生化实验

HSP70 ELISA kit
Widely used ELISA HSP70 kit for chaperone and stress research.
•Quantitative – save time, money, and precious sample with fully quantitative results compared to Western blot analysis
•Specific – antibodies do not react with other HSP
•Validated – tested in stressed and non-stressed samples
The HSP70 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours. Absorbance is read at 450 nm. This kit is ideal for measuring intracellular HSP70 (HSP72). Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
Product Specification
Alternative Name: Heat shock protein 70, HSP72
Sensitivity: 200 pg/ml (range 780 – 50,000 pg/ml) 
Assay Time: 4.5 hours 
Application: For the quantitative determination of HSP70 in cell lysates and tissue of human, mouse, and rat origin. Prod. No. ADI-EKS-715 is recommended for the detection of Hsp70 in serum and plasma samples.
Use/Stability: Store all components at +4°, except standard at -20°.
Kit/Set Contains: Microtiter plate, 5X Extraction reagent, Standard, Sample diluent 2, Wash buffer concentrate, Antibody, Conjugate, TMB Substrate, Stop solution 2
Miscellaneous/General: The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

+4°

ADI-EKS-750   Anti-HSP70 IgG/A/M (human), ELISA kit   品牌 Enzo

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Anti-HSP70 IgG/A/M (human), ELISA kit

货号： ADI-EKS-750      产品名称： Anti-HSP70 IgG/A/M (human), ELISA kit   品牌： Enzo 规格： 1 x 96 wells 3周到货 生化实验

Anti-HSP70 IgG/A/M (human), ELISA kit
Most comprehensive portfolio of heat shock protein (chaperone) products commercially available.
•Quantitative – fully quantitate levels of auto-antibodies to HSP70
•Higher throughput – obtain results from up to 41 samples in duplicate in less than 4 hours
•Easy-to-use – liquid color-coded reagents and pre-coated plates reduce errors and save time in the lab
Product Specification
Alternative Name: Heat shock protein 70, HSP72 
Sensitivity: 6.79 ng/ml (range 31.25 – 1,000 ng/ml)  
Assay Time: <4 hours
Application: For the quantitative determination of anti-HSP70 IgG/A/M autoantibodies in human serum. 
Use/Stability: Store all components at +4°, except standard at -20°. 
Kit/Set Contains: Microtiter plate, Standard, Sample diluent 2, Wash buffer concentrate, HRP Conjugate, TMB Substrate, Stop solution 2  
Miscellaneous/General: The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

+4°