Anti-Ras, clone EP1125Y

货号： 04-1039      产品名称： Anti-Ras, clone EP1125Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Ras/p21, clone EP1125Y, Rabbit Monoclonal
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PRODUCT FAMILY INFORMATION
RAS Antibodies, Kits, Proteins & Plasmids
Millipore’s RAS antibodies, kits, and plasmids are high quality, validated tools for research. These are based on the expertise of Upstate & Chemicon. See below for Millipore’s Ras G-protein products.
Millipore’s RAS antibodies, kits, plasmids and proteins are high quality, validated tools for RAS research. See below for data and references for Millipore’s RAS products. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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p21 Antibodies, Kits & Assays
Millipore’s p21 antibodies, kits and assays have been well validated and published. See below for a comprehensive list of our p21 products, based on the expertise of Upstate & Chemicon.
Millipore’s p21 antibodies, kits and assays are based on the expertise of Upstate & Chemicon. Our p21 reagents have been well validated and published. See the ordering tab for a comprehensive list of our p21 products and direct links to their technical pages for data, references and related products.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, FC, IP Rabbit Unpurified Monoclonal Antibody
Description:
Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal
Promotional Text:
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Specificity:
This antibody detects H-Ras, N-Ras and K-Ras at and around the N-terminus.
Molecular Weight:
~21 kDa
Epitope:
N-terminus
Immunogen:
Synthetic peptide corresponding to residues near the N-terminal region of human Ras.
Clone:
EP1125Y
Isotype:
IgG
Background Information:
Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and Ral GDS.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, and rat.
Application Notes:
Flow Cytometry Analysis: Flow cytometric analysis of permeabilized PC-12 cells using Anti-Ras (1:100 dilution).
Immunoprecipitation: A 1:30 dilution from a previous lot was used in IP.
Control:
C6 cell lysate
Quality Assurance:
Evaluated by Western Blot on C6 cell lysates.
Western Blot Analysis: A 1:500,000 dilution of this antibody was used to detect Ras in C6 cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may -20°C

anti-Ras (H-,N-), clone 1A6.2

货号： 05-1071      产品名称： anti-Ras (H-,N-), clone 1A6.2   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Ras Antibody, ( H-, N-), clone 1A6.2
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-Ras Antibody, ( H-, N-), clone 1A6.2
Promotional Text:
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes H- and N-Ras, NOT K-Ras.
Molecular Weight:
21 kDa
Immunogen:
Full length recombinant GST-tagged human H-Ras.
Clone:
1A6.2
Isotype:
IgG1κ
Background Information:
Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in there approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and RalGDS. One path that the pharmaceutical industry has taken to control Ras and its activity is by finding what some consider its Achilles’ heel. For its activation, Ras must localize to the plasma membrane, but interestingly, it lacks a transmembrane domain. To achieve this, Ras must first undergo a post-translational modification (PTM) known as prenylation or geranylation at its C-terminal CAAX motif. For this to take place, a controlled three step process must occur. The first step in the process is the prenylation or geranylation of the C in the CAAX motif that is initiated by the covalent attachment of farnesyl groups to the cysteine that is catalyzed by the heterodimer enzymes farnesyl transferases and . After this modification, the –aaX of the motif is proteolytically removed via Rce1 (Ras Converting Enzyme 1), a membrane associated endoprotease, by a mechanism that is still not fully understood. Finally, the C-terminal prenylcysteine is now methlylated by ICMT (Isoprenylcysteine Carboxymethyl Transferase). These drugs have yet to pass clinical trials though and there is doubt that they will ever be successful in treating tumors associated with Ras activation.
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Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysate from human A431 carcinoma cells, mouse 3T3, mouse brain, orrat brain.
Purification Method:
Protein G purfied
Presentation:
Protein G purified mouse monoclonal in storage buffer containing PBS and 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 4°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
P01116
Entrez Gene Number:
NM_005343
Gene Symbol:
K-Ras
Ki-Ras
K-Ras2
Kras-2
p21B
KRAS
RASK2
HRAS
HA_RAS
N-RAS
H-RAS
NRAS
NRAS1
ALPS4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not t +4°C

anti-Ras (K-,H-,N-), clone 9A11.2

货号： 05-1072      产品名称： anti-Ras (K-,H-,N-), clone 9A11.2   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2
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Special Shipping Offer on Antibodies
100% Performance Guaranteed
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes K-, H-, and N-Ras (all 3 isofroms).
Molecular Weight:
21 kDa
Immunogen:
Full length recombinant GST-tagged human H-Ras.
Clone:
9A11.2
Isotype:
IgG1κ
Background Information:
Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in there approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and RalGDS. One path that the pharmaceutical industry has taken to control Ras and its activity is by finding what some consider its Achilles’ heel. For its activation, Ras must localize to the plasma membrane, but interestingly, it lacks a transmembrane domain. To achieve this, Ras must first undergo a post-translational modification (PTM) known as prenylation or geranylation at its C-terminal CAAX motif. For this to take place, a controlled three step process must occur. The first step in the process is the prenylation or geranylation of the C in the CAAX motif that is initiated by the covalent attachment of farnesyl groups to the cysteine that is catalyzed by the heterodimer enzymes farnesyl transferases and . After this modification, the –aaX of the motif is proteolytically removed via Rce1 (Ras Converting Enzyme 1), a membrane associated endoprotease, by a mechanism that is still not fully understood. Finally, the C-terminal prenylcysteine is now methlylated by ICMT (Isoprenylcysteine Carboxymethyl Transferase). These drugs have yet to pass clinical trials though and there is doubt that they will ever be successful in treating tumors associated with Ras activation.
View All »
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysate from human A431 carcinoma cells, mouse 3T3, mouse brain, orrat brain.
Purification Method:
Protein G purfied
Presentation:
Protein G purified mouse monoclonal in storage buffer containing 0.1M Tris-Glycine (pH 7.4), 15mM NaCl, and 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 4°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
P01116
Entrez Gene Number:
NM_005343
Gene Symbol:
K-Ras
Ki-Ras
K-Ras2
Kras-2
p21B
KRAS
RASK2
HRAS
HA_RAS
N-RAS
H-RAS
NRAS
NRAS1
ALPS4
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our pr +4°C