标签归档：raf

7726-100   RAF1, Active   品牌 Biovision

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RAF1, Active

货号： 7726-100      产品名称： RAF1, Active   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Active RAF1
A proto-oncogene
Product Overview
Alternate Names / Synonyms: raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase
Gene Symbol: RAF1
Accession #: P04049
Gene ID: 5894
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Molecular Weight: 63.0 kDa
Purity by SDS-PAGE: ≥85%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

7726-5   RAF1, Active   品牌 Biovision

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RAF1, Active

货号： 7726-5      产品名称： RAF1, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active RAF1
A proto-oncogene
Product Overview
Alternate Names / Synonyms: raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase
Gene Symbol: RAF1
Accession #: P04049
Gene ID: 5894
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Molecular Weight: 63.0 kDa
Purity by SDS-PAGE: ≥85%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

475958-20MG   C-Raf Inhibitor   品牌 Merck

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C-Raf Inhibitor

货号： 475958-20MG      产品名称： C-Raf Inhibitor   品牌： Merck 规格： *** 3周到货 生化实验

c-Raf Inhibitor
1-(3-(1,4-dihydroimidazo[4,5-c]pyrazol-5-yl)-4-methylphenyl)-3-(3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)phenyl)urea
A cell-permeable pyrazoloimidazole phenyl urea derivative that acts as a selective type II inhibitor of c-Raf. It shows an IC50 value of 50 nM in an enzymatic assay, and comparable anti-proliferative activities to sorafenib against A375P (GI 50 = 2.68 µM) and WM3629 (GI50 = 0.86 µM) human melanoma cell lines. When screening against a panel of 30 different kinases at a concentration of 10 µM, it exerts an inhibitory activity of 96% on c-Raf, but below 50% for most of the other kinases.
产品信息
Form  Off white solid
Molar mass  480.45
Purity  ≥95% by HPLC
Solubility  DMSO
Chemical formula  C23H19F3N8O
商店和运输信息
Storage  +2°C to +8°C
Ship  Ambient Temperature Only
Standard Handling

2°C – 8°C

521238-10MG   PDGFRbeta/RAF Kinase Inhibitor   品牌 Merck

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PDGFRbeta/RAF Kinase Inhibitor

货号： 521238-10MG      产品名称： PDGFRbeta/RAF Kinase Inhibitor   品牌： Merck 规格： *** 3周到货 生化实验

PDGFRβ/RAF Kinase Inhibitor
6-(4-(5-(3-(Trifluoromethyl)phenylamino)-4 H-1,2,4-triazol-3-yl)phenoxy)-2-(methylthio)pyrimidin-4-amine , PDGFR Tyrosine Kinase Inhibitor XXII
An orally bioavailable amino-triazole compound that is shown to bind to the inactive conformations of both PDGFRβ and Raf kinases (allosteric, Type II inh), and acts as a potent inhibitor of Flt3, Kit, PDGFRα and PDGFRβ (Kd = 52, 170, 300 and 520 nM, respectively) with selectivity over B-Raf, p38α, FGFR2 and VEGFR2 (Kd > 10 µM) in a multi-kinase panel. Completely blocks the phosphorylations of MEK-1/2 S217/S221, ERK-1/2 T202/Y204 in FGF-stimulated HUVECs, PDGFRβ in PDGF-BB-stimulated VSMCs, ERK-1/2 T202/Y204 in 1205Lu-B Raf V600E cells, and C-Raf S338 at 10 µM. Reduces FGF-induced angiogenesis in mouse Matrigel model (50 mg/kg, i.p., bid) and selectively induces apoptosis in the endothelium; further, suppresses orthotopic renal cell carcinoma growth in mice (100 mg/kg, p.o., qd).
产品信息
Form  Beige solid
Molar mass  459.6
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥95% by HPLC
Solubility  DMSO
Chemical formula  C20H16F3N7OS
商店和运输信息
Storage  -20°C
Ship  Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling

-20°C