标签归档：raf

04-412   anti-Raf1, Rb mono   品牌 Millipore

发表于由

anti-Raf1, Rb mono

货号： 04-412      产品名称： anti-Raf1, Rb mono   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Raf-1 Antibody, rabbit monoclonal
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REFERENCES
Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor.
Edin, Matthew L and Juliano, Rudy L (2005) Mol. Cell. Biol., 25: 4466-75 (2005)
Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding
Zhu, Jun, et al (2005) Mol Biol Cell, 16:4733-44 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IF, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-Raf-1 Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Raf-1.
Molecular Weight:
74 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the central region of human Raf-1.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Suggested Dilutions:
Immunoblot Analysis: 1:500
Immunoprecipitation: 1:100
Immunohistochemistry (PE): 1:50 – 1:100
Immunfluorescence Staining: 1:50 – 1:100
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
View All »
UniProt Number:
P04049
Entrez Gene Number:
NM_002880.2
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
View All »
Alternate Names:
RAF1, CRAF, c-Raf, v-raf-1 murine leukemia viral oncogene homolog 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
View All »
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino aci -20°C

04-739   ANTI-RAF-1,! PC 12-305 100ul   品牌 Millipore

发表于由

ANTI-RAF-1,! PC 12-305 100ul

货号： 04-739      产品名称： ANTI-RAF-1,! PC 12-305 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Raf-1, clone AM223
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IP, WB Rabbit null Monoclonal Antibody
Description:
Anti-Raf-1 Antibody, clone AM223 | 04-739
Promotional Text:
Special Offer on Antibodies! Click Here!
Specificity:
Recognizes Raf-1, Mr 74 kDa.
Molecular Weight:
74 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated, synthetic peptide corresponding to the C-terminus (amino acids 637-648 (CTLTTSPRLPVF)) of human Raf-1.
Clone:
AM223
Isotype:
IgG
Background Information:
The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a c-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. In addition to its role in mitogenesis, Raf-1 may play a role in regulation of apoptosis and cell cycle progression. Activation of Raf-1 involves phosphorylation of Ser338/339 and Tyr340/341. Activating mutations of B-Raf that disrupt its auto-inhibition loop have been implicated in a number of cancers, including melanoma and colon cancer.
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and mouse. Predicted to cross-react with rat based on sequence homology.
Application Notes:
Western Blotting Analysis:
A 1:500-1:2,000 diltuoin of this lot detected Raf-1 in 3T3/A31 cell lysates.
Immunoprecipitation: 2-4 μg of a previous lot immunoprecipitated Raf-1 from 400 μg of 3T3/A31 RIPA lysate.
Control:
3T3/A31 cell lysate.
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
Routinely evaluated by Western Blot on 3T3/A31 lysates.
Presentation:
Cultured supernatant with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P04049
Entrez Gene Number:
NM_002880
Gene Symbol:
C-RAF
CRAF
EC 2.7.11.1
NS5
RAF
Raf-1
c-Raf
cRaf
View All »
Alternate Names:
Oncogene RAF1
raf proto-oncogene serine/threonine protein kinase
v-raf-1 murine leukemia viral oncogene homolog 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificitypro -20°C

05-534   ANTI-PHOSPHO-RAF-1 (SER338)100UG   品牌 Millipore

发表于由

ANTI-PHOSPHO-RAF-1 (SER338)100UG

货号： 05-534      产品名称： ANTI-PHOSPHO-RAF-1 (SER338)100UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-Raf-1 (Ser338) Antibody
Close
REFERENCES
Activation of protein kinase Calpha signaling prevents cytotoxicity and mutagenicity following lead acetate in CL3 human lung cancer cells.
Chun-Yu Wang, Yun-Wei Lin, Jia-Ling Yang (2008) Toxicology.250:55-61
Raf kinase as a target for anticancer therapeutics.
Sridhar, Srikala S, et al. (2005) Mol. Cancer Ther., 4: 677-85 (2005)
Renewing the conspiracy theory debate: does Raf function alone to mediate Ras oncogenesis
Repasky, Gretchen A, et al. (2004) Trends Cell Biol., 14: 639-47 (2004)
The RAF proteins take centre stage.
Wellbrock, Claudia, et al. (2004) Nat. Rev. Mol. Cell Biol., 5: 875-85 (2004)
View All
Species Reactivity Key Applications Host Format Antibody Type
M  WB Rat Purified Monoclonal Antibody
Description:
Anti-phospho-Raf-1 (Ser338) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Raf-1 phosphorylated at Ser 338, irrespective of the phosphorylation state of Tyr341; does not bind to unactive Raf-1 or Raf-1 peptides phosphorylated only at Tyr341 or Ser339
Molecular Weight:
74kDa
Immunogen:
Peptide corresponding to residues 334-347 of Raf-1; recognizes phosphorylated Raf-1 on Serine 338.
Modifications:
Phosphorylation
Isotype:
IgG1
Species Reactivity:
Mouse
Quality Assurance:
routinely evaluated by immunoblot on PDGF stimulated 3T3/NIH cells
Purification Method:
Protein A chromatography
Presentation:
PBS, pH 7.4, with 0.09% sodium azide
Storage Conditions:
1 year at -20°C
UniProt Number:
P04049
Entrez Gene Number:
NM_002880
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
View All »
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-es -20°C

05-538   ANTI-PHOS-RAF-1 (S338) 200UG   品牌 Millipore

发表于由

ANTI-PHOS-RAF-1 (S338) 200UG

货号： 05-538      产品名称： ANTI-PHOS-RAF-1 (S338) 200UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-Raf-1 (Ser338) Antibody
Close
REFERENCES
Regulation of mTORC1 signaling by pH.
Aruna D Balgi,Graham H Diering,Elizabeth Donohue,Karen K Y Lam,Bruno D Fonseca,Carla Zimmerman,Masayuki Numata,Michel Roberge (2011) PloS one.6
Development of a highly reproducible three-dimensional organotypic model of the oral mucosa.
Anna Dongari-Bagtzoglou, Helena Kashleva (2006) Nature protocols.1:2012-8
Raf kinase as a target for anticancer therapeutics.
Sridhar, Srikala S, et al. (2005) Mol. Cancer Ther., 4: 677-85 (2005)
Renewing the conspiracy theory debate: does Raf function alone to mediate Ras oncogenesis
Repasky, Gretchen A, et al. (2004) Trends Cell Biol., 14: 639-47 (2004)
View All
Species Reactivity Key Applications Host Format Antibody Type
M  WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Raf-1 (Ser338) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Raf-1 phosphorylated at Serine 338.
Molecular Weight:
74 kDa
Immunogen:
Peptide corresponding to residues 333-345 of human Raf-1
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Mouse
Control:
Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with PDGF
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P04049
Entrez Gene Number:
NM_002880
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
View All »
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protei 2°C – 8°C

05-538SP   ANTI-PHOS-RAF-1 (S338)   品牌 Millipore

发表于由

ANTI-PHOS-RAF-1 (S338)

货号： 05-538SP      产品名称： ANTI-PHOS-RAF-1 (S338)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-Raf-1 (Ser338) Antibody
Close
REFERENCES
Regulation of mTORC1 signaling by pH.
Aruna D Balgi,Graham H Diering,Elizabeth Donohue,Karen K Y Lam,Bruno D Fonseca,Carla Zimmerman,Masayuki Numata,Michel Roberge (2011) PloS one.6
Development of a highly reproducible three-dimensional organotypic model of the oral mucosa.
Anna Dongari-Bagtzoglou, Helena Kashleva (2006) Nature protocols.1:2012-8
Raf kinase as a target for anticancer therapeutics.
Sridhar, Srikala S, et al. (2005) Mol. Cancer Ther., 4: 677-85 (2005)
Renewing the conspiracy theory debate: does Raf function alone to mediate Ras oncogenesis
Repasky, Gretchen A, et al. (2004) Trends Cell Biol., 14: 639-47 (2004)
View All
Species Reactivity Key Applications Host Format Antibody Type
M  WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Raf-1 (Ser338) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Raf-1 phosphorylated at Serine 338.
Molecular Weight:
74 kDa
Immunogen:
Peptide corresponding to residues 333-345 of human Raf-1
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Mouse
Control:
Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with PDGF
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P04049
Entrez Gene Number:
NM_002880
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
View All »
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protei 2-8℃

07-1343   Anti-phopsho-B-Raf (Ser445)   品牌 Millipore

发表于由

Anti-phopsho-B-Raf (Ser445)

货号： 07-1343      产品名称： Anti-phopsho-B-Raf (Ser445)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-B-Raf (Ser445)
Species Reactivity Key Applications Host Format Antibody Type
M, R, H, Xn, Ch, Ca, Eq  WB, EA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-B-Raf (Ser445) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Antibody recognizes B-Raf phosphorylated at and around Ser445.
Molecular Weight:
84 kDa
Epitope:
Phosphorylated Ser445
Immunogen:
KLH-conjugated linear peptide corresponding to human B-Raf phosphorylated at and around Ser445.
Modifications:
Phosphorylation
Background Information:
The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a C-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. B-Raf acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. B-Raf is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. B-Raf acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.
View All »
Species Reactivity:
Mouse
Rat
Human
Xenopus
Chicken
Canine
Horse
View All »
Species Reactivity Note:
Reacts with mouse and rat. Predicted to react with human, xenopus, chicken, dog and horse based on 100% sequence homology.
Control:
NIH/3T3 untreated and PDGF treated cell lysates.
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 untreated and PDGF treated cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected phosphorylated B-Raf (Ser445) on NIH/3T3 cell lysates.
Purification Method:
Affinity Purfied
Presentation:
Purified Rabbit Serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P15056
Entrez Gene Number:
NP_004324
Gene Symbol:
BRAF
Alternate Names:
B-RAF
B-Raf
BRAF1
p94
Raf
RAF
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Enzyme Assay
Entrez Gene Summary:
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma o 2°C – 8°C

07-396   ANTI-RAF-1 200UG PC 12-305   品牌 Millipore

发表于由

ANTI-RAF-1 200UG PC 12-305

货号： 07-396      产品名称： ANTI-RAF-1 200UG PC 12-305   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Raf-1 Antibody
Close
REFERENCES
Dectin-1 directs T helper cell differentiation by controlling noncanonical NF-kappaB activation through Raf-1 and Syk.
Sonja I Gringhuis,Jeroen den Dunnen,Manja Litjens,Michiel van der Vlist,Brigitte Wevers,Sven C M Bruijns,Teunis B H Geijtenbeek (2009) Nature immunology.10
Raf kinase as a target for anticancer therapeutics.
Sridhar, Srikala S, et al. (2005) Mol. Cancer Ther., 4: 677-85 (2005)
Renewing the conspiracy theory debate: does Raf function alone to mediate Ras oncogenesis
Repasky, Gretchen A, et al. (2004) Trends Cell Biol., 14: 639-47 (2004)
The RAF proteins take centre stage.
Wellbrock, Claudia, et al. (2004) Nat. Rev. Mol. Cell Biol., 5: 875-85 (2004)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB Rabbit Purified Polyclonal Antibody
Description:
Anti-Raf-1 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Raf-1
Molecular Weight:
70kDa
Immunogen:
peptide corresponding to amino acids 637-648 of human Raf-1.
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from 3T3/A31 cell lysates
Purification Method:
Protein A purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20degC
UniProt Number:
P04049
Entrez Gene Number:
NM_002880
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
View All »
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
View All &ra -20℃

07-453   ANTI-B-RAF 200UG   品牌 Millipore

发表于由

ANTI-B-RAF 200UG

货号： 07-453      产品名称： ANTI-B-RAF 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-B-Raf Antibody
Close
REFERENCES
A Micro-RNA Connection in BRaf(V600E)-Mediated Premature Senescence of Human Melanocytes.
Gang Ren,Jingwei Feng,Ila Datar,Aaron H Yeung,Srinivas Vinod Saladi,Yongqing Feng,Ivana de la Serna,Kam C Yeung (2012) International journal of cell biology.2012
Filamin A-mediated down-regulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cells.
Tie-Nian Zhu,Hua-Jun He,Sutapa Kole,Theresa D’Souza,Rachana Agarwal,Patrice J Morin,Michel Bernier (2007) The Journal of biological chemistry.282
Lack of association between BRAF V600E mutation and mitogen-activated protein kinase activation in papillary thyroid carcinoma.
Hui Zuo,Yasushi Nakamura,Hironao Yasuoka,Ping Zhang,Misa Nakamura,Ichiro Mori,Akira Miyauchi,Kennichi Kakudo (2007) Pathology international.57
Raf kinase as a target for anticancer therapeutics.
Sridhar, Srikala S, et al. (2005) Mol. Cancer Ther., 4: 677-85 (2005)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IP, WB Rabbit Purified Polyclonal Antibody
Description:
Anti-B-Raf Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
full length recombinant BRaf (plus 6His tag) and B-Raf splice variants in cell lysates
Molecular Weight:
94kDa
Immunogen:
synthetic peptide corresponding to amino acids 754-765 of human B-Raf
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme
Purification Method:
Protein A chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
P15056
Entrez Gene Number:
NM_004333
Gene Symbol:
BRAF
B-raf-1
BRAF1
MGC126806
B-Raf
p94
MGC138284
RAFB1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehe -20℃

ALX-270-324-M001   Raf1 Kinase Inhibitor I   品牌 Alexis

发表于由

Raf1 Kinase Inhibitor I

货号： ALX-270-324-M001      产品名称： Raf1 Kinase Inhibitor I   品牌： Alexis 规格： 1 mg 3周到货 生化实验

Raf1 Kinase Inhibitor I 
Potent Raf1 kinase inhibitor (IC50=9nM). Shows ≥100-fold selectivity for Raf kinase versus CDK1, CDK2, c-Src, ERK2, MEK, p38, Tie2, VEGFR2, and c-Fms.
Product Specification
Alternative Name: 5-Iodo-3-[(3,5-dibromo-4-hydroxyphenyl)methylene]-2-indolinone, GW 5074
Formula: C15H8Br2INO2
MW: 520.9
CAS: 220904-83-6
Purity: ≥97% (HPLC)
Appearance: Off-white to orange powder.
Solubility: Soluble in DMSO (200mg/ml).
Long Term Storage: -20°C
Use/Stability: Stock solutions are stable for at least 6 months when stored at -20°C.
Handling: Protect from light. Keep under inert gas.

-20°C

07-583   ANTI-B-RAF, NT 200UL   品牌 Millipore

发表于由

ANTI-B-RAF, NT 200UL

货号： 07-583      产品名称： ANTI-B-RAF, NT 200UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-B-Raf Antibody, NT
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REFERENCES
Rap1/B-Raf signaling is activated in neuroendocrine tumors of the digestive tract and Raf kinase inhibition constitutes a putative therapeutic target.
Dorothee Karhoff,Susanne Sauer,Jörg Schrader,Rudolf Arnold,Volker Fendrich,Detlef K Bartsch,Dieter Hörsch (2007) Neuroendocrinology.85
Raf kinase as a target for anticancer therapeutics.
Sridhar, Srikala S, et al. (2005) Mol. Cancer Ther., 4: 677-85 (2005)
The role of B-RAF in melanoma.
Gray-Schopfer, Vanessa C, et al. (2005) Cancer Metastasis Rev., 24: 165-83 (2005)
Renewing the conspiracy theory debate: does Raf function alone to mediate Ras oncogenesis
Repasky, Gretchen A, et al. (2004) Trends Cell Biol., 14: 639-47 (2004)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  EA, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-B-Raf Antibody, NT
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
B-Raf
Molecular Weight:
95kDa
Immunogen:
synthetic peptide corresponding to amino acids 10-22 of human B-Raf
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from MCF-7 and PC12 cells
Purification Method:
Immunoaffinity purified
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
P15056
Entrez Gene Number:
NM_004333
Gene Symbol:
BRAF
B-raf-1
BRAF1
MGC126806
B-Raf
p94
MGC138284
RAFB1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Enzyme Assay
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. &amp -20℃