RAF1, Active

货号： 7726-100      产品名称： RAF1, Active   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Active RAF1
A proto-oncogene
Product Overview
Alternate Names / Synonyms: raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase
Gene Symbol: RAF1
Accession #: P04049
Gene ID: 5894
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Molecular Weight: 63.0 kDa
Purity by SDS-PAGE: ≥85%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C