标签归档：rab

ABN38   Anti-RAB35   品牌 Millipore

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Anti-RAB35

货号： ABN38      产品名称： Anti-RAB35   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-RAB35
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Species Reactivity Key Applications Host Format Antibody Type
H, R, Ca, Eq  WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-RAB35 Antibody
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Specificity:
This antibody recognizes RAB35 at the C-terminus.
Molecular Weight:
~19 kDa observed. The calculated molecular weight is 23 kDa.
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of human RAB35.
Background Information:
RAB35 is a member of the Rab GTPases identified in the human genome. It is ubiquitously expressed and regulates a fast endocytotic recycling events associated with the terminal steps of cytokinesis, the formation of immunological synapse, and peptide loading onto major histocompatibility complex III molecules. RAB35 also plays a significant role in the formation of the immunological synapse (IS) between peptide-loaded antigen presenting cells (APCs) and T cells.
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Species Reactivity:
Human
Rat
Canine
Horse
Species Reactivity Note:
Demonstrated to react with Human and Rat. Predicted to react with Canine and Equine based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RAB35 in normal human brain tissue.
Control:
Human brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in human brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected RAB35 on 10 µg of human brain tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q15286
Entrez Gene Number:
NP_006852
Gene Symbol:
RAB35
RAB1C
RAY
Alternate Names:
Ras-related protein Rab-35
GTP-binding protein RAY
Ras-related protein Rab-1C
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
SUBUNIT STRUCTURE: Interacts with DENND1A and DENND1B in a nucleotide-dependent manner. The low binding to DENND1C is nucleotide-independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Membrane › clathrin-coated pit. Cytoplasmic vesicle › clathrin-coated vesicle. Endosome. Melanosome. Note: Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and p 2-8℃

ABS93   Anti-RAB25   品牌 Millipore

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Anti-RAB25

货号： ABS93      产品名称： Anti-RAB25   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-RAB25
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, B, Eq  WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-RAB25 Antibody
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Molecular Weight:
~25 kDa observed.
Uncharacterized bands may be visible at ~60 and ~72 kDa in some lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human RAB25.
Background Information:
Ras-related protein Rab-25 (RAB25) is a GTP-binding protein belonging to the Rab family of proteins. RAB25 has been observed to be expressed in kidney, lung and gastrointestinal mucosa, with exclusive expression in epithelial cells. This specific expression has led to the implication that RAB25 has a role as a regulator for vesicular transport associated with epithelia. RAB25 is potentially a selective regulator for transcytotic pathways and apical recycling. Several recent studies have observed a loss of RAB25 in breast cancer tissue and cell lines, and have suggested a potential role for RAB25 as a regulator for Ras induced tumorigenesis.
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Species Reactivity:
Human
Mouse
Rat
Bovine
Horse
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Species Reactivity Note:
Demonstrated to react with Human, Mouse and Rat.
Predicted to react with Bovine and Equine based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: A 1:600 dilution of a representative lot of this antibody detected RAB25 in invasive and in situ ductal carcinoma tissue.
Control:
MCF7 cell lysate
Quality Assurance:
Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected RAB25 in 10 µg of MCF7 cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P57735
Entrez Gene Number:
NP_065120
Gene Symbol:
RAB25
CATX8
Alternate Names:
Ras-related protein Rab-25
CATX-8
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
Summary: RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM].
UniProt Summary:
FUNCTION: Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling and/or transcytotic pathways.
SUBUNIT STRUCTURE: Interacts with RAB11FIP1, RAB 2-8℃