Anti-RAB35

货号： ABN38      产品名称： Anti-RAB35   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-RAB35
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Species Reactivity Key Applications Host Format Antibody Type
H, R, Ca, Eq  WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-RAB35 Antibody
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Specificity:
This antibody recognizes RAB35 at the C-terminus.
Molecular Weight:
~19 kDa observed. The calculated molecular weight is 23 kDa.
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of human RAB35.
Background Information:
RAB35 is a member of the Rab GTPases identified in the human genome. It is ubiquitously expressed and regulates a fast endocytotic recycling events associated with the terminal steps of cytokinesis, the formation of immunological synapse, and peptide loading onto major histocompatibility complex III molecules. RAB35 also plays a significant role in the formation of the immunological synapse (IS) between peptide-loaded antigen presenting cells (APCs) and T cells.
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Species Reactivity:
Human
Rat
Canine
Horse
Species Reactivity Note:
Demonstrated to react with Human and Rat. Predicted to react with Canine and Equine based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RAB35 in normal human brain tissue.
Control:
Human brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in human brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected RAB35 on 10 µg of human brain tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q15286
Entrez Gene Number:
NP_006852
Gene Symbol:
RAB35
RAB1C
RAY
Alternate Names:
Ras-related protein Rab-35
GTP-binding protein RAY
Ras-related protein Rab-1C
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
SUBUNIT STRUCTURE: Interacts with DENND1A and DENND1B in a nucleotide-dependent manner. The low binding to DENND1C is nucleotide-independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Membrane › clathrin-coated pit. Cytoplasmic vesicle › clathrin-coated vesicle. Endosome. Melanosome. Note: Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and p 2-8℃