标签归档：fak

PS1008-100UL   PD ANTI-FAK (PTYR861) RABBIT PAB   品牌 Merck

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PD ANTI-FAK (PTYR861) RABBIT PAB

货号： PS1008-100UL      产品名称： PD ANTI-FAK (PTYR861) RABBIT PAB   品牌： Merck 规格： *** 3周到货 生化实验

PS1008  PhosphoDetect™ Anti-FAK (pTyr86¹) Rabbit pAb
Anti-FADK1, Phospho-Specific (Tyr86¹), Anti-pp125FAK, Phospho-Specific (Tyr86¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr86¹), Anti-PTK2, Phospho-Specific (Tyr86¹), FAK 1
Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic phosphopeptide surrounding the Tyr86¹ phosphorylation site of human FAK
Form  Liquid
Formulation  In Dulbecco’s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
Species reactivity  chicken, human, mouse
Preservative  ≤0.1 sodium azide
Applications 
Immunoblotting (Western Blotting)
Immunohistochemistry
Avoid freeze/thaw  是的
Positive control  Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
商店和运输信息
Storage  -20°C
Ship  Shipped with Blue Ice or with Dry Ice
Irritant

-20°C

V1971   FAK Kinase Enzyme System   品牌 Promega

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FAK Kinase Enzyme System

货号： V1971      产品名称： FAK Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 His 标签的重组人源 FAK （第 393 位 – 第 698 位氨基酸 ） 。 FAK （ Focal Adhesion Kinase ， 黏着斑激酶）是一种非受体型蛋白酪氨酸激酶，参与了介导细胞与胞外基质联系的整合素富集黏着斑位点的信号转导。 FAK 增强的信号分子可以介导贴壁依赖性细胞的存活，在生长因子受体和整合素刺激引起的有效细胞迁移中至关重要。在人类肿瘤中，表达量升高的 FAK 与恶化程度和侵染程度的增强均相关。在间变型星形细胞瘤和胶质母细胞瘤的活组织切片样本中，已证明伴随有 FAK 表达量的升高。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量：ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： FAK ， 10μg ( 人源，重组， 第 393 位 – 第 698 位氨基酸 ) ； MW : ~35kDa 。
•  底物： Poly (4:1 Glu, Tyr) Peptide 。
•  其它组分： 反应缓冲液， DTT 。
FAK NCBI 数据库入口。
人类激酶酶系统及其应用。
特点  •  在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2 。
•  可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 
 

05-537NA-KL   Anti-FAK, PBS only   品牌 Millipore

发表于由

Anti-FAK, PBS only

货号： 05-537NA-KL      产品名称： Anti-FAK, PBS only   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-FAK Antibody, clone 4.47
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REFERENCES
Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is critical for formation of α-smooth muscle actin filaments during myofibroblast differentiation.
Cai, Guo-Qiang, et al. (2012) Am. J. Physiol. Lung Cell Mol. Physiol., 303: L692-702 (2012)
ADAM15 Protein Amplifies Focal Adhesion Kinase Phosphorylation under Genotoxic Stress Conditions.
Dorothee Fried,Beate B Böhm,Kristin Krause,Harald Burkhardt (2012) The Journal of biological chemistry.287
Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo.
Hicham Lahlou,Virginie Sanguin-Gendreau,Margaret C Frame,William J Muller (2012) Breast cancer research : BCR.14
Dual targeting of EGFR and focal adhesion kinase in 3D grown HNSCC cell cultures.
Eke I, Cordes N (2011) Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.99:279-86. Epub 2011 Jun 23.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  ICC, IHC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-FAK Antibody, clone 4.47
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes p125FAK; does not cross react with Pyk-2.
Molecular Weight:
125 kDa
Immunogen:
GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK
Clone:
4.47
Isotype:
IgG1
Background Information:
Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.
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Species Reactivity:
Human
Mouse
Rat
Control:
Positive Control Included: 3T3 Cell Lysate (12-305). Other controls: A31 cell lysate, PC-12, Hep-G2, SW 620 and Jurkat RIPA cell lysates
Quality Assurance:
Routinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Q05397
Entrez Gene Number:
NM_005607 / NM_153831
Gene Symbol:
PTK2
pp125FAK
FAK
FADK
FAK1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are no -20℃

BML-SE448-0010   FAK (human) (recombinant)   品牌 Biomol

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FAK (human) (recombinant)

货号： BML-SE448-0010      产品名称： FAK (human) (recombinant)   品牌： Biomol 规格： 10 ug 3周到货 生化实验

FAK (human), (recombinant) (His-tag)
Product Specification
Alternative Name:Focal adhesion kinase
MW:36 kDa
Source:Produced in insect cells. Active FAK (aa 411-686).
UniProt ID:Q05397
Application:Kinetic and functional studies, phosphorylation of target substrates, drug screening.
Long Term Storage:-80°C
Miscellaneous/General:FAK is involved in cell motility and it concentrates in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents such as fibronectin. It is also a major target for the tyrosine kinase Src and its deletion can block tumor formation and progression.

-80°C

ABT135   Anti-phospho-FAK (Tyr397)   品牌 Millipore

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Anti-phospho-FAK (Tyr397)

货号： ABT135      产品名称： Anti-phospho-FAK (Tyr397)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-FAK (Tyr397)
Species Reactivity Key Applications Host Format Antibody Type
R, H, Xn, Ch, M  WB, Cell Function Assay Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-FAK (Tyr397) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes FAK phosphorylated at Tyr397.
Molecular Weight:
~125 kDa observed.
An uncharacterized band appears at ~190 kDa in some lysates.
Epitope:
Phosphorylated Tyr397
Immunogen:
KLH-conjugated linear peptide corresponding to human FAK phosphorylated at Tyr397.
Modifications:
Phosphorylation
Background Information:
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.
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Species Reactivity:
Rat
Human
Xenopus
Chicken
Mouse
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Species Reactivity Note:
Demonstrated to react with Rat.
Predicted to react with Human, Xenopus, Chicken, and Mouse based on 100% sequence homology.
Application Notes:
Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot peptide blocked on RAT2 cells transfected with Src.
Control:
untransfected and Src-transfected RAT2 cell lysates
Quality Assurance:
Evaluated by Western Blot in untransfected and Src-transfected RAT2 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-FAK (Tyr397) in 10 µg of untransfected and Src-transfected RAT2 cell lysates.
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Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q05397
Entrez Gene Number:
NP_722560
Gene Symbol:
PTK2
FAK
FAK1
Alternate Names:
Focal adhesion kinase 1
FADK 1
Protein-tyrosine kinase 2
pp125FAK
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Cell Function Assay
Entrez Gene Summary:
This gene encodes a cytoplasmic protein tyros 2-8℃