Anti-FAK, PBS only

货号： 05-537NA-KL      产品名称： Anti-FAK, PBS only   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-FAK Antibody, clone 4.47
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REFERENCES
Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is critical for formation of α-smooth muscle actin filaments during myofibroblast differentiation.
Cai, Guo-Qiang, et al. (2012) Am. J. Physiol. Lung Cell Mol. Physiol., 303: L692-702 (2012)
ADAM15 Protein Amplifies Focal Adhesion Kinase Phosphorylation under Genotoxic Stress Conditions.
Dorothee Fried,Beate B Böhm,Kristin Krause,Harald Burkhardt (2012) The Journal of biological chemistry.287
Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo.
Hicham Lahlou,Virginie Sanguin-Gendreau,Margaret C Frame,William J Muller (2012) Breast cancer research : BCR.14
Dual targeting of EGFR and focal adhesion kinase in 3D grown HNSCC cell cultures.
Eke I, Cordes N (2011) Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.99:279-86. Epub 2011 Jun 23.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  ICC, IHC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-FAK Antibody, clone 4.47
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes p125FAK; does not cross react with Pyk-2.
Molecular Weight:
125 kDa
Immunogen:
GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK
Clone:
4.47
Isotype:
IgG1
Background Information:
Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.
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Species Reactivity:
Human
Mouse
Rat
Control:
Positive Control Included: 3T3 Cell Lysate (12-305). Other controls: A31 cell lysate, PC-12, Hep-G2, SW 620 and Jurkat RIPA cell lysates
Quality Assurance:
Routinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Q05397
Entrez Gene Number:
NM_005607 / NM_153831
Gene Symbol:
PTK2
pp125FAK
FAK
FADK
FAK1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are no -20℃