Anti-phospho-FAK (Tyr397), clone EP2160Y

货号： 04-974      产品名称： Anti-phospho-FAK (Tyr397), clone EP2160Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, R  WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody detects FAK phosphorylated at Tyr397.
Molecular Weight:
~ 125 kDa
Epitope:
Tyr397
Immunogen:
Phospho-specific peptide surrounding tyrosine 397 of human FAK.
Modifications:
Phosphorylation
Clone:
EP2160Y
Isotype:
IgG
Background Information:
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human and rat.
Control:
Human and rat brain lysates
Quality Assurance:
Evaluated by Western Blot on Human and rat brain cell lysates.
Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect FAK in human and rat brain cell lysates.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q05397
Entrez Gene Number:
Q05397
Gene Symbol:
EC 2.7.10.2
FADK
FAK
FAK1
pp125FAK
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Alternate Names:
FADK 1
PTK2 protein tyrosine kinase 2
Protein-tyrosine kinase 2
focal adhesion kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, prolife -20°C

anti-phospho-FAK (Tyr407)

货号： 07-829      产品名称： anti-phospho-FAK (Tyr407)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-FAK (Tyr407) Antibody
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REFERENCES
Expression of GRP and its receptor in well-differentiated colon cancer cells correlates with the presence of focal adhesion kinase phosphorylated at tyrosines 397 and 407
Matkowskyj, Kristina A, et al (2003) J Histochem Cytochem, 51:1041-8 (2003)
Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities
Le Boeuf, Fabrice, et al (2004) J Biol Chem, 279:39175-85 (2004)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-FAK (Tyr407) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphor-FAK (Tyr407)
Molecular Weight:
125 kDa
Immunogen:
Peptide corresponding to amino acid region encompassing the human, mouse, rat, and chicken phospho-FAK (Tyr407)
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Predicted to cross-react with Mouse, Rat, and Chicken based on complete sequence homology.
Application Notes:
Outside independent labs report it working in immunocytochemistry and immunohistochemistry.
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Immunoaffinity purified
Storage Conditions:
2 years at -20°C
UniProt Number:
Q05397
Entrez Gene Number:
NM_005607.3
NM_153831.2
Gene Symbol:
PTK2
pp125FAK
FAK1
FADK
FAK
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Alternate Names:
PTK2, FADK, FAK, FAK1, pp125FAK, Focal Adhesion Kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
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UniProt Summary:
FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Intera -20℃

anti-phospho-FAK (Tyr576)

货号： 07-830      产品名称： anti-phospho-FAK (Tyr576)   品牌： Millipore 规格： EA

anti-phospho-FAK (Tyr861)

货号： 07-832      产品名称： anti-phospho-FAK (Tyr861)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-FAK (Tyr861) Antibody
Species Reactivity Key Applications Host Format Antibody Type
Ch, H, M  WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-FAK (Tyr861) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated FAK (Tyr861)
Molecular Weight:
125 kDa
Immunogen:
Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-FAK (Tyr861)
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Chicken
Human
Mouse
Species Reactivity Note:
Predicted to cross-react with rat based on sequence homology (100%), but have not been tested.
Purification Method:
Affinity Purfied
Storage Conditions:
2 years at -20°C
UniProt Number:
Q05397
Entrez Gene Number:
NM_005607.3
NM_153831.2
Gene Symbol:
PTK2
pp125FAK
FAK1
FADK
FAK
Alternate Names:
Focal Adhesion Kinase, pp125FAK, FADK1, PTK2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
UniProt Summary:
FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase fam -20℃