标签归档：src

BML-P312-0001   pp60v-src Autophosphorylation Site (pY Substrate)   品牌 Biomol

发表于由

pp60v-src Autophosphorylation Site (pY Substrate)

货号： BML-P312-0001      产品名称： pp60v-src Autophosphorylation Site (pY Substrate)   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This sequence, RRLIEDNEpYTARG, is the v-Src (412-422) autophosphorylation site and this peptide contains a phosphorylated tyrosine. Useful in a nonradioactive tyrosine phosphatase assay and as a competitive tyrosine phosphatase inhibitor.
Product Specification
Sequence:Arg-Arg-Leu-Ile-Glu-Asp-Asn-Glu-pTyr-Thr-Ala-Arg-Gly
MW:1671.9
Long Term Storage:-20°C

-20°C

539571-5MG   InSolution Src Inhibitor, PP1   品牌 Merck

发表于由

InSolution Src Inhibitor, PP1

货号： 539571-5MG      产品名称： InSolution Src Inhibitor, PP1   品牌： Merck 规格： *** 3周到货 生化实验

InSolution™ Src Inhibitor, PP1
A cell-permeable pyrazolopyrimidine compound that is shown to inhibit Src family tyrosine kinases Lck, Fyn, Hck, and Src (IC50 = 5, 6, 20, and 170 nM, respectively), as well as non-Src family kinases such as CSK, RIP2, and CK1δ (IC50 = 640 nM, 26 nM, 170 nM, respectively), in in vitro kinase assays. At 1 µM, in vitro kinase activity is reduced by more than 57% for p38α, p38β, CK1δ, CSK, RIP2, and GAK, while exhibiting much lower activity against more than 60 other kinases. Kinetic studies employing Src and [poly Glu-Tyr 4:1] indicate that the inhibition is mixed competitive with respect to the substrate and non-competitive with respect to ATP. PP1 also demonstrates anti-growth properties in several B lymphoma cultures of human and murine origins (IC50 = 2.5 to 8.0 µM) and pediatric brain tumor cells (IC50 = 1.1 to 9.7 µM). 1 mg of Src Inhibitor, PP1 (Cat. No. 567809) is also available.
产品信息
Form  Liquid
Formulation  A 50 mM (5 mg/355 µl) solution of Src Inhibitor, PP1 (Cat. No. 567809) in DMSO.
Molar mass  281.36
Protect from Light  是的
Hygrocopic  是的
Avoid freeze/thaw  是的
Packaged under inert gas  是的
Purity  ≥90% by HPLC
Chemical formula  C16H19N5
商店和运输信息
Storage  -20°C
Ship  Shipped with Blue Ice or with Dry Ice
Irritant

-20°C

567809-1MG   Src Inhibitor PP1   品牌 Merck

发表于由

Src Inhibitor PP1

货号： 567809-1MG      产品名称： Src Inhibitor PP1   品牌： Merck 规格： *** 3周到货 生化实验

Src Inhibitor, PP1
4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo-d-3,4-pyrimidine
A cell-permeable pyrazolopyrimidine compound that is shown to inhibit Src family tyrosine kinases Lck, Fyn, Hck, and Src (IC50 = 5, 6, 20, and 170 nM, respectively), as well as non-Src family kinases such as CSK, RIP2, and CK1δ (IC50 = 640 nM, 26 nM, 170 nM, respectively), in in vitro kinase assays. At 1 µM, in vitro kinase activity is reduced by more than 57% for p38α, p38β, CK1δ, CSK, RIP2, and GAK, while exhibiting much lower activity against more than 60 other kinases. Kinetic studies employing Src and [poly Glu-Tyr 4:1] indicate that the inhibition is mixed competitive with respect to the substrate and non-competitive with respect to ATP. PP1 also demonstrates anti-growth properties in several B lymphoma cultures of human and murine origins (IC50 = 2.5 to 8.0 µM) and pediatric brain tumor cells (IC50 = 1.1 to 9.7 µM). Also available in InSolution™ format (Cat. No. 539741).
产品信息
Form  Off-white solid
Molar mass  281.36
Purity  ≥90% by HPLC
Solubility  DMSO
Chemical formula  C16H19N5
CAS number  172889-26-8
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C

BML-SA413-0020   [pTyr529]Src, pAb   品牌 Biomol

发表于由

[pTyr529]Src, pAb

货号： BML-SA413-0020      产品名称： [pTyr529]Src, pAb   品牌： Biomol 规格： 20 ul 3周到货 生化实验

[pTyr529]Src, pAb
Product Specification
Alternative Name:Proto-oncogene c-Src, pp60c-src
Host:Rabbit
Immunogen:Synthetic peptide corresponding to a portion of human Src phosphorylated at Tyr529.
UniProt ID:P12931
Application:Western Blot
Specificity:Recognizes human, mouse, rat and chicken src phosphorylated at Tyr529.
Formulation:Liquid. In PBS, pH 7.3.
Long Term Storage:-80°C

-80°C

BML-SE357-0005   Src (human) (recombinant)   品牌 Biomol

发表于由

Src (human) (recombinant)

货号： BML-SE357-0005      产品名称： Src (human) (recombinant)   品牌： Biomol 规格： 5 ug 3周到货 生化实验

Src (human), (recombinant) (GST-tag)
Product Specification
Alternative Name:pp60src
MW:~83kDa
Source:Produced in E. coli.
UniProt ID:P12931
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Purity:≥95% (SDS-PAGE)
Application:Kinetic and functional studies, phosphorylation of target substrates, drug screening.
Long Term Storage:-80°C
Use/Stability:After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Miscellaneous/General:Src, a non-receptor type tyrosine kinase, is a proto-oncogene that plays key roles in cell morphology, motility, proliferation, and survival. Src is overexpressed and activated in a large number of human cancers. Oncogenic forms of Src alter cell structure and affect thin filaments and adhesion networks that control cell migration. Src also influences the RhoA-ROCK pathway that controls actin polymerization, the STAT family of transcription factors which are involved in tumorigenesis and the Cbl ubiquitin ligase that controls Src protein levels.

-80°C

07-1285   Anti-acetylated-Src (Lys430)   品牌 Millipore

发表于由

Anti-acetylated-Src (Lys430)

货号： 07-1285      产品名称： Anti-acetylated-Src (Lys430)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-acetylated-Src (Lys430)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, Cell Function Assay Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetylated-Src (Lys430) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Src acetylated at Lys430.
Molecular Weight:
~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the weight of the protein.
Epitope:
Acetylated Lys430
Immunogen:
KLH-conjugated linear peptide corresponding to human Src acetylated at Lys430.
Modifications:
Acetylation
Background Information:
Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Rat and Mouse based on 100% sequence homology.
Application Notes:
Western Blot Analysis: 5 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.
Control:
HEK293T cells transfected with myc-tagged Src and CBP
Quality Assurance:
Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P12931
Entrez Gene Number:
NP_005408
Gene Symbol:
Src1
c-src
p60-Src
SRC1
c-SRC
Alternate Names:
SRC
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Cell Function Assay
UniProt Summary:
SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Product Name:
Anti-acetylated-Src (Lys430)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
This Anti-acetylated-Src (Lys430) Antibody is validated for use in WB, Cell Funct 2°C – 8°C

07-1322   Anti-acetylated-Src (Lys7)   品牌 Millipore

发表于由

Anti-acetylated-Src (Lys7)

货号： 07-1322      产品名称： Anti-acetylated-Src (Lys7)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-acetylated-Src (Lys7)
Species Reactivity Key Applications Host Format Antibody Type
H, R, M  WB, Cell Function Assay Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetylated-Src (Lys7) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Src acetylated at Lys7.
Molecular Weight:
~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the molecular weight of the protein.
Epitope:
Acetylated Lys7
Immunogen:
KLH-conjugated linear peptide corresponding to human Src acetylated at Lys7.
Modifications:
Acetylation
Background Information:
Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.
View All »
Species Reactivity:
Human
Rat
Mouse
Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Rat and Mouse based on 100% sequence homology.
Application Notes:
Western Blot Analysis: 2 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.
Control:
HEK293T cells transfected with myc-tagged Src and CBP
Quality Assurance:
Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P12931
Entrez Gene Number:
NP_005408
Gene Symbol:
Src1
c-src
p60-Src
SRC1
c-SRC
View All »
Alternate Names:
SRC
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Cell Function Assay
UniProt Summary:
SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
View All »
Product Name:
Anti-acetylated-Src (Lys7)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applicat 2°C – 8°C

ABC105   Anti-Phospho-Src (Tyr419)   品牌 Millipore

发表于由

Anti-Phospho-Src (Tyr419)

货号： ABC105      产品名称： Anti-Phospho-Src (Tyr419)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Phospho-Src (Tyr419)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Phospho-Src (Tyr419) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Src phosphorylated at Tyr419.
Molecular Weight:
~60 kDa observed
Epitope:
Phosphorylated Tyr419
Immunogen:
KLH-conjugated linear peptide corresponding to human Src phosphorylated at Tyr419.
Background Information:
Src is a ubiquitous non-receptor tyrosine kinase with relatively high levels of expression in neurons, osteoclasts, and platelets. It couples many different classes of cell surface receptors, including immune receptors, integrins, receptor tyrosine kinases, and cytokine receptors, to their respective intracellular signaling networks. Src recognizes activated cell surface receptors as dimers or clusters, and phosphorylates their tyrosine residues, providing binding motifs for signaling molecules. By this mechanism, Src facilitates a number of signaling pathways involved in cell adhesion, cell cycle, apoptosis, gene transcription, and cell migration. Src may also play a role in tumor development. Src is regulated by phosphorylation at multiple tyrosine residues.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Mouse and Rat based on 100% sequence homology.
Control:
COS1 transfected with wildtype Src
Quality Assurance:
Evaluated by Western Blot/Peptide Inhibition Assay in COS1 transfected with wildtype Src.
Western Blot Analysis: 0.02 µg/mL of this antibody detected Src on 10 µg of COS1 transfected with wildtype Src.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P12931
Entrez Gene Number:
NP_005408
Gene Symbol:
SRC
SRC1
Alternate Names:
Proto-oncogene tyrosine-protein kinase Src
Proto-oncogene c-Src
pp60c-src
p60-Src
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Peptide Inhibition Assay
Product Name:
Anti-Phospho-Src (Tyr419)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Phospho-Src (Tyr419) using this Anti-Phospho-Src (Tyr419) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.

2-8℃

12-140   SRC SBST PPT,P34 (CDC2)(6-20) 1MG   品牌 Millipore

发表于由

SRC SBST PPT,P34 (CDC2)(6-20) 1MG

货号： 12-140      产品名称： SRC SBST PPT,P34 (CDC2)(6-20) 1MG   品牌： Millipore 规格： EA 询盘 生化实验

Src Substrate Peptide
Close
PRODUCT FAMILY INFORMATION
Kinase & Phosphatase Products
Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Millipore has a broad and continuously expanding portfolio of recombinant proteins. This portfolio includes more than 350 active, inactive and unactivated kinases, phosphatases and the largest available selection of disease relevant mutants. All of our enzymes are available in quantities sufficient for routine testing, as well as high throughput screening.
» More…
Description:
Src Substrate Peptide
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 mg
Product Overview:
Synthetic peptide (KVEKIGEGTYGVVYK) corresponding to amino acids 6-20 of p34cdc2.
Applications:
Src Substrate Peptide primarily used in Kinase Assays.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
View All »
UniProt Summary:
SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
View All »
Molecular Weight:
1670 Daltons (1.67 kDa)
Brand Family:
Upstate
Protein Target:
Src
Presentation:
Lyophilized powder
Storage Conditions:
Stable for 2 years at -20°C
Target Sub-Family:
TK
Gene Symbol:
SRC
ASV
c-src
p60-Src
SRC1
c-SRC
pp60c-src
View All »
Product Name:
Src Substrate Peptide
Quality Assurance:
Routinely evaluated in a protein kinase assay as a substrate for Src (p60c-src), (14-117) or Lck (p56lck), active (14-379).
UniProt Number:
P12931
Entrez Gene Number:
NM_005417.3
NM_198291.1

-20℃

12-543   SRC SUBS PEP, BIOTIN CONJ 1MG   品牌 Millipore

发表于由

SRC SUBS PEP, BIOTIN CONJ 1MG

货号： 12-543      产品名称： SRC SUBS PEP, BIOTIN CONJ 1MG   品牌： Millipore 规格： EA 三周到货 生化实验

Src Substrate Peptide, biotin conjugate
Close
PRODUCT FAMILY INFORMATION
Kinase & Phosphatase Products
Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Millipore has a broad and continuously expanding portfolio of recombinant proteins. This portfolio includes more than 350 active, inactive and unactivated kinases, phosphatases and the largest available selection of disease relevant mutants. All of our enzymes are available in quantities sufficient for routine testing, as well as high throughput screening.
» More…
Description:
Src Substrate Peptide, biotin conjugate
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 mg
Applications:
Src Substrate Peptide, biotin conjugate primarily used in Kinase Assays.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
View All »
UniProt Summary:
SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
View All »
Brand Family:
Upstate
Protein Target:
Src
Presentation:
Lyophilized powder
Storage Conditions:
1 year at 4°C
Target Sub-Family:
TK
Gene Symbol:
SRC
ASV
c-src
p60-Src
SRC1
c-SRC
pp60c-src
View All »
Product Name:
Src Substrate Peptide, biotin conjugate
Quality Assurance:
Routinely evaluated as a substrate for Src, active (Catalog # 14-326).
UniProt Number:
P12931
Entrez Gene Number:
NM_005417.3
NM_198291.1

4℃