标签归档：src

PK1109-50UL   PD ANTI-SRC FAMILY (PTYR416) RABBIT PAB   品牌 Merck

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PD ANTI-SRC FAMILY (PTYR416) RABBIT PAB

货号： PK1109-50UL      产品名称： PD ANTI-SRC FAMILY (PTYR416) RABBIT PAB   品牌： Merck 规格： *** 3周到货 生化实验

PhosphoDetect™ Anti-Src Family (pTyr4¹6) Rabbit pAb
Recognizes the ~60 kDa Src protein phosphorylated at Tyr416 in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr4¹6 phosphorylation site of human Src
Form  Liquid
Formulation  In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Species reactivity Xenopus, chicken, human, mouse, rat
Preservative  None
Applications 
Immunoblotting (Western Blotting)
Positive control  COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

-20°C

V2921   SRC Kinase Enzyme System   品牌 Promega

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SRC Kinase Enzyme System

货号： V2921      产品名称： SRC Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明 
 我们在大肠杆菌（E. coli）中表达 N 端带 GST 标签的重组全长人源 SRC 。 SRC 家族属于非受体酪氨酸激酶，最初被认为是 劳斯氏肉瘤病 毒（RSV）的转化蛋白，可以催化蛋白底物中的酪氨酸发生磷酸化。 SRC 在许多人类恶性肿瘤中过表达并被活化，与癌症的发生和远处转移酶的发展相关联。除了能促进细胞增殖外， SRC 在癌症中的一个关键作用是促进侵入和运动，这些功能可能导致了肿瘤的发展。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量：ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： SRC ，10μg ( 人源，重组全长蛋白) ； MW : ~83kDa 。
•  底物： SRC 底物（KVEKIGEGTYGVVYK-amide ）；从人 p34cdc2 （第6 位–第20 位氨基酸 ) 衍生得到。
•  其它组分： 反应缓冲液，DTT ，MnCl2 。
SRC NCBI 数据库入口。
人类激酶酶系统及其应用。
特点  •  在实验室内即可筛选更多的化合物：ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2 。
•  可得到可靠结果：ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 

7750-5   Src1, Active   品牌 Biovision

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Src1, Active

货号： 7750-5      产品名称： Src1, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active Src1
A proto-oncogene
Product Overview
Product Name: Active Src1
Alternate Names / Synonyms: SRC 1, SRC-1, Active Src1, Src1, Src, human Src1, active kinase, active kinases, recombinant Src1, recombinant Src, kinase activity assay, protein kinase.
Gene Symbol: SRC
Accession #: P12931
Gene ID: 6714
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 85.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 116 nmol phosphate incorporated into Src substrate peptide per minute per mg proteins at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83µCi/assay in 25µl reaction volume).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: Src family belongs to non-receptor tyrosine kinases. Src was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. Src is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression. Oncogenic forms of the Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that Src plays a role in these functions by influencing the RhoA-ROCK pathway that controls contractile actin filament assembly, the STAT family of transcription factors needed for transformation, and the Cbl ubiquitin ligase that controls Src protein levels. These studies also shed light on the role of focal adhesion kinase (FAK) downstream of v-Src and other signaling pathways in controlling migration, invasion and survival of transformed cells. Src directly phosphorylates integrins and can also modulate R-Ras activity. Moreover, it stimulates the E-cadherin regulator Hakai, interacts with and phosphorylates the novel podosome-linked adaptor protein Fish, and progressively phosphorylates the gap junction component connexion 43. In addition to the above functions and substrates, the role in cellular physiology of Src is continually expanding.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

7750-100   Src1, Active   品牌 Biovision

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Src1, Active

货号： 7750-100      产品名称： Src1, Active   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Active Src1
A proto-oncogene
Product Overview
Product Name: Active Src1
Alternate Names / Synonyms: SRC 1, SRC-1, Active Src1, Src1, Src, human Src1, active kinase, active kinases, recombinant Src1, recombinant Src, kinase activity assay, protein kinase.
Gene Symbol: SRC
Accession #: P12931
Gene ID: 6714
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 85.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 116 nmol phosphate incorporated into Src substrate peptide per minute per mg proteins at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83µCi/assay in 25µl reaction volume).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: Src family belongs to non-receptor tyrosine kinases. Src was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. Src is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression. Oncogenic forms of the Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that Src plays a role in these functions by influencing the RhoA-ROCK pathway that controls contractile actin filament assembly, the STAT family of transcription factors needed for transformation, and the Cbl ubiquitin ligase that controls Src protein levels. These studies also shed light on the role of focal adhesion kinase (FAK) downstream of v-Src and other signaling pathways in controlling migration, invasion and survival of transformed cells. Src directly phosphorylates integrins and can also modulate R-Ras activity. Moreover, it stimulates the E-cadherin regulator Hakai, interacts with and phosphorylates the novel podosome-linked adaptor protein Fish, and progressively phosphorylates the gap junction component connexion 43. In addition to the above functions and substrates, the role in cellular physiology of Src is continually expanding.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

BML-P197-0001   Src Peptide Substrate   品牌 Biomol

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Src Peptide Substrate

货号： BML-P197-0001      产品名称： Src Peptide Substrate   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This peptide, KVEKIGEGTYGVVYK, is derived from amino acids 6-20 of cdc2 and is a good substrate for src and other tyrosine kinases.
Product Specification
Alternative Name:cdc2 (6-20)
Sequence:Lys-Val-Glu-Lys-Ile-Gly-Glu-Gly-Thr-Tyr-Gly-Val-Val-Tyr-Lys
MW:1671
Purity:95%
Long Term Storage:-20°C

-20°C

BML-P259-0001   Src Peptide Substrate II   品牌 Biomol

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Src Peptide Substrate II

货号： BML-P259-0001      产品名称： Src Peptide Substrate II   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This peptide, YIYGSFK, was derived through a random combinatorial peptide library method and is a specific and efficient substrate for the scr family of kinases. The Km of YIYGSFK for p60c-src is 55µM.
Product Specification
Alternative Name:Tyr-Ile-Tyr-Gly-Ser-Phe-Lys
Sequence:H-Tyr-Ile-Tyr-Gly-Ser-Phe-Lys-OH
MW:877.3
Formulation:Lyophilized.
Purity:≥95% (HPLC)
Solubility:Soluble in water.
Long Term Storage:-20°C

-20°C

BML-P260-0001   Src Peptide Substrate (Biotinylated)   品牌 Biomol

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Src Peptide Substrate (Biotinylated)

货号： BML-P260-0001      产品名称： Src Peptide Substrate (Biotinylated)   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This peptide, YIYGSFK, was derived through a random combinatorial peptide library method and is a specific and efficient substrate for the scr family of kinases. The Km of YIYGSFK for p60c-src is 55µM.
Product Specification
Alternative Name:Tyr-Ile-Tyr-Gly-Ser-Phe-Lys
Sequence:H-Tyr-Ile-Tyr-Gly-Ser-Phe-Lys-OH
MW:877.3
Formulation:Lyophilized.
Purity:≥95% (HPLC)
Solubility:Soluble in water.
Long Term Storage:-20°C

-20°C

BML-P301-0001   pp60c-Src C-terminal Phosphoregulatory Peptide   品牌 Biomol

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pp60c-Src C-terminal Phosphoregulatory Peptide

货号： BML-P301-0001      产品名称： pp60c-Src C-terminal Phosphoregulatory Peptide   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This phosphopeptideis the C-terminal negative regulatory domain of c-Src (521-533) which includes the negative regulatory site (Tyr527). This peptide, when tyrosine-phosphorylated, binds to the internal SH2 domain.
Product Specification
Alternative Name:[pTyr527] c-Src (521-533)
Sequence:Ac-Thr-Ser-Thr-Glu-Pro-Gln-pTyr-Gln-Pro-Gly-Glu-Asn-Leu
MW:1585.7
Purity:>98%
Appearance:Lyophilized solid.
Solubility:Soluble in Water.
Long Term Storage:-20°C

-20°C

BML-P304-0001   pp60c-Src C-terminal Peptide   品牌 Biomol

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pp60c-Src C-terminal Peptide

货号： BML-P304-0001      产品名称： pp60c-Src C-terminal Peptide   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This sequence, TSTEPQYQPGENL, is identical to pp60c-srv, C-terminal Phosphoregulatory Peptide, which includes the negative regulatory site (Tyr527) but is not phosphorylated. Useful as a control peptide or as a tyrosine kinase substrate. This peptide, when tyrosine-phosphorylated, binds to the internal SH2 domain.
Product Specification
Sequence:Thr-Ser-Thr-Glu-Pro-Gln-Tyr-Gln-Pro-Gly-Glu-Asn-Leu
MW:1463.7
Long Term Storage:-20°C

-20°C

BML-P307-0001   pp60v-src Autophosphorylation Site   品牌 Biomol

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pp60v-src Autophosphorylation Site

货号： BML-P307-0001      产品名称： pp60v-src Autophosphorylation Site   品牌： Biomol 规格： 1 mg 3周到货 生化实验

Residues 2-12 are the v-Src (412-422) autophosphorylation site with an extra Arg for phosphocellulose binding. This substrate sequence, RRLIEDNEYTARG, was originally described as an EGFR substrate in A431 cell extracts (Km=0.3-0.6 mM).
Product Specification
Sequence:Arg-Arg-Leu-Ile-Glu-Asp-Asn-Glu-Tyr-Thr-Ala-Arg-Gly
MW:1592.9
CAS:81493-98-3
Purity:97%
Long Term Storage:-20°C

-20°C

BML-P308-0001   RR-src (Protein Tyrosine Kinase Inhibitor)   品牌 Biomol

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RR-src (Protein Tyrosine Kinase Inhibitor)

货号： BML-P308-0001      产品名称： RR-src (Protein Tyrosine Kinase Inhibitor)   品牌： Biomol 规格： 1 mg 3周到货 生化实验

Product Specification
Alternative Name:v-src autophosphorylation site
Sequence:Arg-Arg-Leu-Ile-Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Gly
MW:1519.9
CAS:81156-93-6
Purity:97%
Appearance:Lyophilized solid.
Solubility:Soluble in Water.
Long Term Storage:-20°C

-20°C