A ACETYLATED HISTONE H4 200UG

货号： 06-598      产品名称： A ACETYLATED HISTONE H4 200UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-acetyl-Histone H4 Antibody
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REFERENCES
Histone deacetylase inhibition decreases cholesterol levels in neuronal cells by modulating key genes in cholesterol synthesis, uptake and efflux.
Nunes, Maria João, et al. (2013) PLoS ONE, 8: e53394 (2013)
hCAF1/CNOT7 regulates interferon signalling by targeting STAT1.
Chapat, Clément, et al. (2013) EMBO J., 32: 688-700 (2013)
A FOXO3-IRF7 gene regulatory circuit limits inflammatory sequelae of antiviral responses.
Vladimir Litvak,Alexander V Ratushny,Aaron E Lampano,Frank Schmitz,Albert C Huang,Ayush Raman,Alistair G Rust,Andreas Bergthaler,John D Aitchison,Alan Aderem (2012) Nature.490
Negative regulation of human growth hormone gene expression by insulin is dependent on hypoxia-inducible factor binding in primary non-tumor pituitary cells.
Vakili, Hana, et al. (2012) J. Biol. Chem., 287: 33282-92 (2012)
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Species Reactivity Key Applications Host Format Antibody Type
T, H  ICC, IP, WB, ChIP Rabbit Purified Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes acetylated histone H4.
Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
Molecular Weight:
10 kDa
Epitope:
a.a. 2-19
Immunogen:
KLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.
Modifications:
Acetylation
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Tetrahymena
Human
Species Reactivity Note:
Human and Tetrahymena. Other species not tested.
Application Notes:
Immunoprecipitation:
5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours.
Immunocytochemistry:
5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS.
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.
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Control:
TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.
Quality Assurance:
Routinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
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Purification Method:
Protein A chromatography
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7 -20°C

Acetylated Histone H4 (LYS 5,8,12,16)

货号： 12-379-K      产品名称： Acetylated Histone H4 (LYS 5,8,12,16)   品牌： Millipore 规格： MG 三周到货 生化实验

Acetyl-Histone H4 (Lys5, 8, 12, 16) Peptide, biotin conjugate
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PRODUCT FAMILY INFORMATION
Histone H4 Antibodies and Proteins
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon.
EMD Millipore’s Histone H4 antibody demonstrates specificity against linker histone H4. See below for data and references for EMD Millipore’s Histone H4 antibodies and proteins. All EMD Millipore antibodies and proteins are based on the expertise of Upstate & Chemicon.
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Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
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Description:
Acetyl-Histone H4 (Lys5, 8, 12, 16) Peptide, biotin conjugate
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Synthetic peptide, containing amino acids 1-18 of Histone H4 followed by a GSGS linker and biotinylated lysine (SGRGACKGGACKGLGAC-KGGAACKRHGSGSK-biotin).
Applications:
Acetyl-Histone H4 (Lys5, 8, 12, 16) Peptide, biotin conjugate primarily used in Immunoprecipitation & Affinity Precipitation Assays.
Key Applications:
Immunoprecipitation
Affinity Precipitation Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
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UniProt Summary:
FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to u -20℃