anti-phospho-PRAS40 (Thr246)

货号： 05-1069      产品名称： anti-phospho-PRAS40 (Thr246)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, IP, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Detects PRAS40 when phosphorylated on Thr246.
Molecular Weight:
40 kDa
Epitope:
Phosphorylated Thr246
Immunogen:
Synthetic peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40.
Modifications:
Phosphorylation
Clone:
4H2.2
Isotype:
IgG1κ
Background Information:
PRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and Mouse. Predicted to cross-react with rat and bovine based sequence homology of immunizing peptide.
Quality Assurance:
Evaluated by western blot on untreated and PDGF-treated NIH/3T3 cell lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect PRAS40 in PDGF-treated NIH/3T3 cell lysate.
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Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q96B36
Entrez Gene Number:
NP_001092102.1
Gene Symbol:
PRAS40
AKT1S1
Lobe
Alternate Names:
40 kDa proline-rich AKT substrate
AKT1 substrate 1 (proline-rich)
proline-rich Akt substrate, 40 kDa
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunocytochemistry
Entrez Gene Summary:
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]
UniProt Summary:
FUNCTION: May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SUBUNIT STRUCTURE: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm – cytosol. Not 2°C – 8°C

anti-phospho-PRAS40 (Thr246)

货号： 07-1282      产品名称： anti-phospho-PRAS40 (Thr246)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-PRAS40 (Thr246)
Species Reactivity Key Applications Host Format Antibody Type
M, R, H, B  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PRAS40 (Thr246) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Detects PRAS40 when phosphorylated on Thr246.
Molecular Weight:
40 kDa
Epitope:
Phosphorylated Thr246
Immunogen:
KLH-conjugated synthetic linear peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40.
Modifications:
Phosphorylation
Background Information:
PRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
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Species Reactivity:
Mouse
Rat
Human
Bovine
Species Reactivity Note:
Rat and Mouse. Predicted to cross-react with human and bovine based sequence homology of immunizing peptide.
Quality Assurance:
Evaluated by western blot on untreated and Insulin-treated NIH/3T3 cell lysate
Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect PRAS40 in Insulin-treated NIH/3T3 cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Antigen affinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
Q96B36
Entrez Gene Number:
NM_026270.3
Gene Symbol:
Lobe
MGC2865
PRAS40
Alternate Names:
40 kDa proline-rich AKT substrate
AKT1 substrate 1 (proline-rich)
proline-rich Akt substrate, 40 kDa
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SUBUNIT STRUCTURE: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm › cytosolBy similarity. Note= Found in the cytosolic fraction of the brain By similarity.
TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell line 2°C – 8°C

anti-phospho-PRAS40 (Thr246)

货号： 07-888      产品名称： anti-phospho-PRAS40 (Thr246)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.
Molecular Weight:
Approx. 40 kDa
Immunogen:
Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).
Modifications:
Phosphorylation
Isotype:
IgG
Background Information:
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse.
Application Notes:
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Control:
PDGF stimulated NIH3T3 cells.
Quality Assurance:
Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
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Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q96B36
Entrez Gene Number:
NP_001092102
Gene Symbol:
Lobe
MGC2865
OTTHUMP00000196757
OTTHUMP00000196758
OTTHUMP00000196759
OTTHUMP00000196760
PRAS40
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Alternate Names:
40 kDa proline-rich AKT substrate
AKT1 substrate 1 (proline-rich)
proline-rich Akt substrate, 40 kDa
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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-20℃