标签归档：rbt

04-571   Caspase-2, Rbt x, 100ul   品牌 Millipore

发表于由

Caspase-2, Rbt x, 100ul

货号： 04-571      产品名称： Caspase-2, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Caspase 2 Antibody, rabbit monoclonal
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REFERENCES
Activation of caspase-2 in apoptosis
Li, H, et al (1997) J Biol Chem, 272:21010-7 (1997)
Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death
Wang, L, et al (1994) Cell, 78:739-50 (1994)
Apoptosis signaling triggered by the marine alkaloid ascididemin is routed via caspase-2 and JNK to mitochondria
Dirsch, Verena M, et al (2004) Oncogene, 23:1586-93 (2004)
Caspase-2-induced apoptosis is dependent on caspase-9, but its processing during UV- or tumor necrosis factor-dependent cell death requires caspase-3
Paroni, G, et al (2001) J Biol Chem, 276:21907-15 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, M  FC, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase 2 Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Caspase-2.
Immunogen:
A synthetic peptide corresponding to N-terminus of human Caspase-2 p15 subunit. The antibody should recognize both pro-form (~50kDa) and p15 cleaved-form of Caspase-2.
Clone:
Y61
Background Information:
Caspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase; in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:500
Flow Cytometry: 1:10
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P42575
Entrez Gene Number:
NM_032982.2
NM_032983.2
Gene Symbol:
CASP2
NEDD2
ICH1
ICH-1L/1S
ICH-1L
NEDD-2
CASP-2
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Flow Cytometry
Western Blotting
Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript var -20°C

04-573   Caspase-8, Rbt x, 100ul   品牌 Millipore

发表于由

Caspase-8, Rbt x, 100ul

货号： 04-573      产品名称： Caspase-8, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Caspase-8 Antibody, rabbit monoclonal
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REFERENCES
Velogenic newcastle disease virus as an oncolytic virotherapeutics: in vitro characterization.
Rajiv Kumar,Ashok K Tiwari,Uttara Chaturvedi,G Ravi Kumar,Aditya P Sahoo,R S Rajmani,Lovleen Saxena,Shikha Saxena,Sangeeta Tiwari,Sudesh Kumar (2012) Applied biochemistry and biotechnology.167
Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
Boldin, M P, et al. (1996) Cell, 85: 803-15 (1996)
Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases
Srinivasula, S M, et al (1996) Proc Natl Acad Sci USA, 93:14486-91 (1996)
FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)
Medema, J P, et al (1997) EMBO J, 16:2794-804 (1997)
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Species Reactivity Key Applications Host Format Antibody Type
H, R  IP, WB, FC Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase-8 Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Rat Caspase-8.
Immunogen:
A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18. The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.
Clone:
E7
Background Information:
Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, Rat (positive control: Hela cell lysate)
Application Notes:
WB: 1:500
Flow Cytometry: 1:20
IP: 1:40
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q14790
Entrez Gene Number:
NM_001080124.1
NM_001080125.1
NM_001228.4
NM_033355.3
NM_033356.3
NM_033358.3
View All »
Gene Symbol:
CASP8
MCH5
MGC78473
CASP-8
MACH
ALPS2B
FLICE
procaspase-8
CAP4
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Flow Cytometry
Entrez Gene Summary:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires -20°C

04-574   Caspase-8 (N Term), Rbt x, 100ul   品牌 Millipore

发表于由

Caspase-8 (N Term), Rbt x, 100ul

货号： 04-574      产品名称： Caspase-8 (N Term), Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Caspase-8 (NT) Antibody, rabbit monoclonal
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REFERENCES
De-ubiquitinating protease USP2a targets RIP1 and TRAF2 to mediate cell death by TNF.
Mahul-Mellier, A-L, et al. (2011) Cell death and differentiation, (2011)
Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
Boldin, M P, et al. (1996) Cell, 85: 803-15 (1996)
Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases
Srinivasula, S M, et al (1996) Proc Natl Acad Sci USA, 93:14486-91 (1996)
FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)
Medema, J P, et al (1997) EMBO J, 16:2794-804 (1997)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB, IH(P), FC Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase-8 (NT) Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human Caspase-8 (N-Term).
Immunogen:
A synthetic peptide corresponding to N-terminal residues of human Caspase-8. The antibody should recognize all splice isoforms of Caspase-8.
Clone:
E6
Background Information:
Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:3000
IHC: 1:100 (Paraffin-embedded human breast adenocarcinoma)
Flow Cytometry: 1:50
IP: 1:50
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q14790
Entrez Gene Number:
NM_001080124.1
NM_001080125.1
NM_001228.4
NM_033355.3
NM_033356.3
NM_033358.3
View All »
Gene Symbol:
CASP8
MCH5
MGC78473
CASP-8
MACH
ALPS2B
FLICE
procaspase-8
CAP4
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Flow Cytometry
Entrez Gene Summary:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic -20°C

04-575   PARP-1 (P116), Rbt x, 100ul   品牌 Millipore

发表于由

PARP-1 (P116), Rbt x, 100ul

货号： 04-575      产品名称： PARP-1 (P116), Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PARP-1 (p116/p25) Antibody, rabbit monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-PARP-1 (p116/p25) Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human PARP-1 (p116/p25).
Immunogen:
A synthetic peptide corresponding to residues before the cleavage site of human PARP-1. The antibody only recognizes full-length form (p116) of PARP-1.
Clone:
Y17
Background Information:
Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
Species Reactivity:
Human
Species Reactivity Note:
Human (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:1000 IP: 1:80
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P09874
Entrez Gene Number:
NM_001618.2
Gene Symbol:
PARP1
NAD
Poly
PPOL
poly(ADP-ribosyl)transferase
PARP-1
PARP
pADPRT-1
ADPRT1
ADPRT
Alternate Names:
poly (ADP-ribose) polymerase-1
ARTD1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
UniProt Summary:
FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
SIZE: 1014 amino acids; 113084 Da
SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & -20°C

04-577   RasGap, Rbt x, 100ul   品牌 Millipore

发表于由

RasGap, Rbt x, 100ul

货号： 04-577      产品名称： RasGap, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-RasGAP Antibody, rabbit monoclonal, clone EP536Y
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REFERENCES
Surviving the kiss of death.
Yang, Jiang-Yan, et al. (2004) Biochem. Pharmacol., 68: 1027-31 (2004)
Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP
Yang, J Y and Widmann, C (2001) Mol Cell Biol, 21:5346-58 (2001)
Partial cleavage of RasGAP by caspases is required for cell survival in mild stress conditions
Yang, Jiang-Yan, et al (2004) Mol Cell Biol, 24:10425-36 (2004)
Impaired Akt activity down-modulation, caspase-3 activation, and apoptosis in cells expressing a caspase-resistant mutant of RasGAP at position 157
Yang, Jiang-Yan, et al (2005) Mol Biol Cell, 16:3511-20 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H, R  ICC, IH(P), WB Rabbit Purified Monoclonal Antibody
Description:
Anti-RasGAP Antibody, rabbit monoclonal, clone EP536Y
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Rat RasGap.
Immunogen:
A synthetic peptide corresponding to residues near the SH3 domain of human RasGAP.
Clone:
EP536Y
Background Information:
RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).
View All »
Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, Rat (positive control: Rat brain lysate)
Application Notes:
WB: 1:2000
IHC: 1:50 (Paraffin-embedded human ovary carcinoma)
ICC: 1:250 – 500 (Hela cells)
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P20936
Entrez Gene Number:
NM_002890.1
NM_022650.1
Gene Symbol:
RASA1
CMAVM
CM-AVM
RASA
PKWS
GAP
p120GAP
RASGAP
RasGAP
DKFZp434N071
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunocytochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP -20°C

04-578   Smac/Diablo, Rbt x, 100ul   品牌 Millipore

发表于由

Smac/Diablo, Rbt x, 100ul

货号： 04-578      产品名称： Smac/Diablo, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Smac/DIABLO Antibody, rabbit monoclonal, clone Y12
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REFERENCES
Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.
Du, C, et al. (2000) Cell, 102: 33-42 (2000)
Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins
Verhagen, A M, et al (2000) Cell, 102:43-53 (2000)
Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain.
Liu, Z, et al. (2000) Nature, 408: 1004-8 (2000)
Structural basis of IAP recognition by Smac/DIABLO.
Wu, G, et al. (2000) Nature, 408: 1008-12 (2000)
Species Reactivity Key Applications Host Format Antibody Type
H, R  FC, IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Smac/DIABLO Antibody, rabbit monoclonal, clone Y12
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Rat Smac/Diablo.
Immunogen:
A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO.
Clone:
Y12
Background Information:
Smac/DIABLO (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI) is localized to the mitochondria and involved in activating programmed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/DIABLO is released from the mitochondria and binds anti-apoptotic IAP proteins, supressing their inhibitory activity and promoting caspase activation (1,2). This interaction involves primarily the amino-terminal residues of Smac/DIABLO with the BIR3 region of the IAP protein (3,4).
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, Rat (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:1,000
IHC: 1:10 (Paraffin-embedded human skin cancer tissue)
ICC: 1:10
Flow Cytometry: 1:10-50
IP: 1:10-80
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q9NR28
Entrez Gene Number:
NM_019887.3
NM_138929.2
NM_138930.2
Gene Symbol:
DIABLO
SMAC
DIABLO-S
FLJ25049
0610041G12Rik
diablo
FLJ10537
SMAC3
View All »
Alternate Names:
Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
View All »
Entrez Gene Summary:
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.
View All &ra -20°C

04-579   Apaf-1, Rbt x, 100ul   品牌 Millipore

发表于由

Apaf-1, Rbt x, 100ul

货号： 04-579      产品名称： Apaf-1, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Apaf-1 Antibody, rabbit monoclonal, clone E38
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REFERENCES
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.
Zou, H, et al. (1997) Cell, 90: 405-13 (1997)
Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation
Saleh, A, et al (1999) J Biol Chem, 274:17941-5 (1999)
Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis
Hu, Y, et al (1999) EMBO J, 18:3586-95 (1999)
Oligomerization and activation of caspase-9, induced by Apaf-1 CARD
Shiozaki, Eric N, et al (2002) Proc Natl Acad Sci USA, 99:4197-202 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Apaf-1 Antibody, rabbit monoclonal, clone E38
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Apaf-1.
Immunogen:
A synthetic peptide corresponding to residues near N-terminus of human Apaf-1.
Clone:
E38
Background Information:
Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: Hela cell lysate)
Application Notes:
WB: 1:500
IHC: 1:50-1:100 (Paraffin-embedded stomach adenocarcinoma)
ICC: 1:50-1:100
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
O14727
Entrez Gene Number:
NM_001160.2
NM_013229.2
NM_181861.1
NM_181868.1
NM_181869.1
View All »
Gene Symbol:
APAF1
DKFZp781B1145
Apaf-1
KIAA0413
CED4
View All »
Alternate Names:
apoptotic protease activating factor 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encod -20°C

04-581   Paxillin (N Term), Rbt x, 100ul   品牌 Millipore

发表于由

Paxillin (N Term), Rbt x, 100ul

货号： 04-581      产品名称： Paxillin (N Term), Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113
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REFERENCES
Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly.
Burridge, K, et al. (1992) J. Cell Biol., 119: 893-903 (1992)
Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL
Salgia, R, et al (1995) J Biol Chem, 270:5039-47 (1995)
Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase
Bellis, S L, et al (1995) J Biol Chem, 270:17437-41 (1995)
Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration
Iwasaki, Teruo, et al (2002) Int J Cancer, 97:330-5 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Paxillin (N-Term).
Immunogen:
A synthetic peptide corresponding to residues in N-terminus of human Paxillin. This antibody detects alpha, beta and gamma isoforms of Paxillin.
Clone:
Y113
Background Information:
Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Positive control: Hela cell lysate
Application Notes:
WB: 1:5,000-10,000
IHC: 1:100 (Paraffin-embedded breast carcinoma)
ICC: 1:250 (Hela cells)
IP: 1:200
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P49023
Entrez Gene Number:
NM_002859.1
Gene Symbol:
PXN
Paxillin.
paxillin
FLJ16691
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
SIZE: 591 amino acids; 64533 Da
SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds -20°C

04-582   PAK1 (N Term), Rbt x, 100ul   品牌 Millipore

发表于由

PAK1 (N Term), Rbt x, 100ul

货号： 04-582      产品名称： PAK1 (N Term), Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PAK1 (NT) Antibody, rabbit monoclonal, clone EP795Y
Close
REFERENCES
p21-activated kinases: three more join the Pak.
Jaffer, Zahara M and Chernoff, Jonathan (2002) Int. J. Biochem. Cell Biol., 34: 713-7 (2002)
Inhibition of myosin light chain kinase by p21-activated kinase
Sanders, L C, et al (1999) Science, 283:2083-5 (1999)
p21-activated protein kinase: a crucial component of morphological signaling
Daniels, R H and Bokoch, G M (1999) Trends Biochem Sci, 24:350-5 (1999)
A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Manser, E, et al (1994) Nature, 367:40-6 (1994)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  FC, IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-PAK1 (NT) Antibody, rabbit monoclonal, clone EP795Y
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat PAK1 (N-Term).
Immunogen:
A synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein.
Clone:
EP795Y
Background Information:
p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Positive controls: Hela, A431, Jurkat, K562, MCF7, Daudi, NIH/3T3, PC12.
Application Notes:
WB: 1:500
IHC: 1:100-250 (Paraffin-embedded human breast carcinoma)
ICC: 1:50-100 (Hela cells)
Flow Cytometry: 1:10
IP: 1:50
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q13153
Entrez Gene Number:
NM_002576.3
Gene Symbol:
PAK1
p65-PAK
MGC130001
Alpha-PAK
MGC130000
PAK-1
PAKalpha
View All »
Alternate Names:
p21-activated kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
View All »
Entrez Gene Summary:
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures hav -20°C

04-583   PAK4 (N Term), Rbt x, 100ul   品牌 Millipore

发表于由

PAK4 (N Term), Rbt x, 100ul

货号： 04-583      产品名称： PAK4 (N Term), Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PAK4 (NT) Antibody
Close
REFERENCES
p21-activated kinases: three more join the Pak.
Jaffer, Zahara M and Chernoff, Jonathan (2002) Int. J. Biochem. Cell Biol., 34: 713-7 (2002)
p21-activated protein kinase: a crucial component of morphological signaling
Daniels, R H and Bokoch, G M (1999) Trends Biochem Sci, 24:350-5 (1999)
The serine/threonine kinase PAK4 prevents caspase activation and protects cells from apoptosis
Gnesutta, N, et al (2001) J Biol Chem, 276:14414-9 (2001)
A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Manser, E, et al (1994) Nature, 367:40-6 (1994)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-PAK4 (NT) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat PAK4 (N-Term).
Immunogen:
A synthetic peptide corresponding to residues in the N-terminus of human PAK4.
Background Information:
p21-activated kinase 4 (PAK4) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK4 has been shown to regulate cytoskeletal organization and to protect cells from apoptosis induced by multiple stimuli, including serum withdrawal, tumor necrosis factor- (TNF) treatment, or UV irradiation (4). PAK4 expression is elevated in many cancer cell lines, and activated PAK4 is highly transforming, suggesting an important role in tumorigenesis (5).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, Rat (positive control: SH-SY-5Y cell lysate)
Application Notes:
WB: 1:1000
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
O96013
Entrez Gene Number:
NM_001014833.1
NM_001014831.2
NM_001014832.1
NM_001014834.2
NM_001014835.1
NM_005884.3
View All »
Gene Symbol:
PAK4
PAK-4
KIAA1142
Alternate Names:
p21-activated kinase 4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinc -20°C

04-584   PAK1, Rbt x, 100ul   品牌 Millipore

发表于由

PAK1, Rbt x, 100ul

货号： 04-584      产品名称： PAK1, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y
Close
REFERENCES
p21-activated kinases: three more join the Pak.
Jaffer, Zahara M and Chernoff, Jonathan (2002) Int. J. Biochem. Cell Biol., 34: 713-7 (2002)
Inhibition of myosin light chain kinase by p21-activated kinase
Sanders, L C, et al (1999) Science, 283:2083-5 (1999)
p21-activated protein kinase: a crucial component of morphological signaling
Daniels, R H and Bokoch, G M (1999) Trends Biochem Sci, 24:350-5 (1999)
A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Manser, E, et al (1994) Nature, 367:40-6 (1994)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat PAK1.
Immunogen:
A synthetic peptide corresponding to residues in the human PAK1 protein.
Clone:
EP656Y
Background Information:
p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Positive control: Hela cell lysate
Application Notes:
WB: 1:10,000-50,000
IHC: 1: 250-500 (Paraffin-embedded human colon cancer)
ICC: 1:250-500 (Hela cells)
IP: 1:50
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q13153
Entrez Gene Number:
NM_002576.3
Gene Symbol:
PAK1
p65-PAK
MGC130001
Alpha-PAK
MGC130000
PAK-1
PAKalpha
View All »
Alternate Names:
p21-activated kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined.
View All »
UniProt Summary:
FUNCTIO -20°C

04-585   Desmin, Rbt x, 100ul   品牌 Millipore

发表于由

Desmin, Rbt x, 100ul

货号： 04-585      产品名称： Desmin, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Desmin Antibody, rabbit monoclonal, clone Y66
Close
REFERENCES
The antiangiogenic 16K prolactin impairs functional tumor neovascularization by inhibiting vessel maturation.
Nguyen, Ngoc-Quynh-Nhu, et al. (2011) PLoS ONE, 6: e27318 (2011)
High level desmin expression depends on a muscle-specific enhancer.
Li, Z L and Paulin, D (1991) J. Biol. Chem., 266: 6562-70 (1991)
Human desmin-coding gene: complete nucleotide sequence, characterization and regulation of expression during myogenesis and development
Li, Z L, et al (1989) Gene, 78:243-54 (1989)
Intermediate filament structure and assembly
Stewart, M (1993) Curr Opin Cell Biol, 5:3-11 (1993)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Desmin Antibody, rabbit monoclonal, clone Y66
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Desmin.
Immunogen:
A synthetic peptide corresponding to C-terminus of human Desmin. Predicted to cross-react with rat, bovine and pig, based on sequence homology.
Clone:
Y66
Background Information:
Desmin are class-III intermediate filaments found in muscle cells, and are expressed during the formation of muscle cells or fibers. Cytoskeletal intermediate filaments (IFs) are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice (1,2). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Desmin mutations have been linked to familial cardiac and skeletal myopathy (3,4).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Positive control: Mouse heart tissue.
Application Notes:
WB: 1:500
IHC: 1:500 (Paraffin-embedded human heart muscle)
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P17661
Entrez Gene Number:
NM_001927.3
Gene Symbol:
DES
CSM2
FLJ41013
FLJ39719
CSM1
FLJ41793
FLJ12025
Desmin.
CMD1I
desmin
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
View All »
UniProt Summary:
FUNCTION: SwissProt: P17661 # Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the -20°C

04-586   Cytokeratin 18, Rbt x, 100ul   品牌 Millipore

发表于由

Cytokeratin 18, Rbt x, 100ul

货号： 04-586      产品名称： Cytokeratin 18, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
Close
REFERENCES
Keratins and the skin.
Fuchs, E (1995) Annu. Rev. Cell Dev. Biol., 11: 123-53 (1995)
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells
Kulesh, D A and Oshima, R G (1988) Mol Cell Biol, 8:1540-50 (1988)
Identification and mutational analysis of the glycosylation sites of human keratin 18
Ku, N O and Omary, M B (1995) J Biol Chem, 270:11820-7 (1995)
Cytokeratin expression, fibrillar organization, and subtle function in liver cells
Marceau, N and Loranger, ABiochem Cell Biol, 73:619-25 ()
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, ICC, IH(P), FC Rabbit Purified Monoclonal Antibody
Description:
Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Cytokeratin 18.
Immunogen:
A synthetic peptide corresponding to residues of human Cytokeratin 18.
Clone:
E431-1
Background Information:
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
View All »
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western Blot: 1:10,000
Immunohistochemistry: 1:100-1:250 (Paraffin-embedded colon carcinoma)
Immunocytochemistry: 1:100
Flow Cytometry: 1:20

Optimal working dilutions must be determined by the end user.
View All »
Control:
Positive control: A431 cell lysate.
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P05783
Entrez Gene Number:
NM_000224.2
NM_199187.1
Gene Symbol:
KRT18
K18
CYK18
Keratin-18
Cytokeratin-18
CK-18
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Flow Cytometry
Entrez Gene Summary:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: P05783 # Involved in the uptake of thrombin-anti -20°C

04-587   Cytokeratin 5, Rbt x, 100ul   品牌 Millipore

发表于由

Cytokeratin 5, Rbt x, 100ul

货号： 04-587      产品名称： Cytokeratin 5, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Cytokeratin 5 Antibody
Close
REFERENCES
Isolation, sequence, and expression of a human keratin K5 gene: transcriptional regulation of keratins and insights into pairwise control.
Lersch, R, et al. (1989) Mol. Cell. Biol., 9: 3685-97 (1989)
Expression of cytokeratin 5 and calretinin in clear-cell renal cell carcinoma
Cid Mouteira, P, et al (2006) Actas urologicas españolas , 30:367-71 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC, IH(P) Rabbit Purified Polyclonal Antibody
Description:
Anti-Cytokeratin 5 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human Cytokeratin 5.
Immunogen:
A synthetic peptide corresponding to residues near the N-terminus of human CK-5.
Background Information:
Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human (positive control: A431 cell lysate)
Application Notes:
WB: 1:1000
IHC: 1:50 – 100 (Paraffin-embedded human transitional carcinoma of bladder)
ICC: 1:50 – 100
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P13647
Entrez Gene Number:
NM_000424.3
Gene Symbol:
KRT5
DDD
Keratin-5
KRT5A
CK-5
CK5
K5
EBS2
Cytokeratin-5
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
View All »
UniProt Summary:
SIZE: 590 amino acids; 62378 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
DISEASE: SwissProt: P13647 # Defects in KRT5 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 131960]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mu -20°C

04-588   Cytokeratin 8, Rbt x, 100ul   品牌 Millipore

发表于由

Cytokeratin 8, Rbt x, 100ul

货号： 04-588      产品名称： Cytokeratin 8, Rbt x, 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Cytokeratin 8 Antibody, rabbit monoclonal, clone E432
Close
REFERENCES
Keratins and the skin.
Fuchs, E (1995) Annu. Rev. Cell Dev. Biol., 11: 123-53 (1995)
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells
Kulesh, D A and Oshima, R G (1988) Mol Cell Biol, 8:1540-50 (1988)
Identification and mutational analysis of the glycosylation sites of human keratin 18
Ku, N O and Omary, M B (1995) J Biol Chem, 270:11820-7 (1995)
Cytokeratin expression, fibrillar organization, and subtle function in liver cells
Marceau, N and Loranger, ABiochem Cell Biol, 73:619-25 ()
Species Reactivity Key Applications Host Format Antibody Type
H, M  FC, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Cytokeratin 8 Antibody, rabbit monoclonal, clone E432
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Cytokeratin 8.
Immunogen:
A synthetic peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8.
Clone:
E432
Background Information:
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: A431 cell lysate)
Application Notes:
WB: 1:500
IHC: 1:250-1:500 (Paraffin-embedded stomach carcinoma)
ICC: 1:50
Flow Cytometry: 1:20
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P05787
Entrez Gene Number:
NM_002273.2
Gene Symbol:
KRT8
Cytokeratin-8
Keratin-8
K2C8
CYK8
CK-8
KO
K8
CK8
CARD2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P05787 # Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
SIZE: 483 amino acids; 53704 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed -20°C