BML-PW0150-0025   Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌 Biomol

Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)

货号: BML-PW0150-0025      产品名称: Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌: Biomol 规格: 25 ug 3周到货 生化实验

Product Specification
Clone:FK2
Host:Mouse
Isotype:IgG1
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
Application:ELISA
Immunohistochemistry
Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species. Does not cross-react with free ubiquitin.
Formulation:Liquid. In PBS.
Use/Stability:Antibody is supplied at a concentration of 1mg/mL. Store at 4ºC. Can be stored at -20ºC if required, but avoid multiple freeze/thawing. Vial contains purified HRP-linked immunoglobulin suspended in phosphate-buffered saline.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:+4°C
Background / Technical Information:FK2 antibody was conjugated to horseradish peroxidase (HRP) by a proprietary method. The HRP-linked FK2 monoclonal antibody (FK2H) gives enhanced western blotting detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains compared to the unconjugated FK2 antibody (PW8810). For western blotting an initial antibody dilution of 1:1000 is recommended, followed by washing and signal detection using appropriate development reagents.
Note: Milk should NOT be used in blocking/antibody binding solutions. 1% BSA in PBS or TBS Tween should be used instead.

+4°C

BML-PW0150-0100   Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌 Biomol

Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)

货号: BML-PW0150-0100      产品名称: Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌: Biomol 规格: 100 ug 3周到货 生化实验

Product Specification
Clone:FK2
Host:Mouse
Isotype:IgG1
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
Application:ELISA
Immunohistochemistry
Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species. Does not cross-react with free ubiquitin.
Formulation:Liquid. In PBS.
Use/Stability:Antibody is supplied at a concentration of 1mg/mL. Store at 4ºC. Can be stored at -20ºC if required, but avoid multiple freeze/thawing. Vial contains purified HRP-linked immunoglobulin suspended in phosphate-buffered saline.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:+4°C
Background / Technical Information:FK2 antibody was conjugated to horseradish peroxidase (HRP) by a proprietary method. The HRP-linked FK2 monoclonal antibody (FK2H) gives enhanced western blotting detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains compared to the unconjugated FK2 antibody (PW8810). For western blotting an initial antibody dilution of 1:1000 is recommended, followed by washing and signal detection using appropriate development reagents.
Note: Milk should NOT be used in blocking/antibody binding solutions. 1% BSA in PBS or TBS Tween should be used instead.

+4°C

BML-PW0755-0025   Mono- and polyubiquitinated conjugates, mAb(FK2) (Biotin conjugate)   品牌 Biomol

Mono- and polyubiquitinated conjugates, mAb(FK2) (Biotin conjugate)

货号: BML-PW0755-0025      产品名称: Mono- and polyubiquitinated conjugates, mAb(FK2) (Biotin conjugate)   品牌: Biomol 规格: 25 ul 3周到货 生化实验

Product Specification
Clone:FK2
Host:Mouse
Isotype:IgG1
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
Source:Purified from tissue culture supernatant. Biotin conjugated chemically in vitro.
Application:Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes polyubiquitinylated and monoubiquitinylated protein conjugates in a wide range of species. Does not cross-react with free ubiquitin.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:+4°C
Long Term Storage:-20°C
Background / Technical Information:The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated (all linkage types) and monoubiquitinylated proteins but not free ubiquitin. FK2 antibody was conjugated to biotin by a proprietary method. The biotin-linked FK2 monoclonal antibody (FK2-biotin) facilitates ready detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains by Western blotting using a Streptavidin-HRP detection system.

-20°C

BML-PW0755-0100   Mono- and polyubiquitinated conjugates, mAb(FK2) (Biotin conjugate)   品牌 Biomol

Mono- and polyubiquitinated conjugates, mAb(FK2) (Biotin conjugate)

货号: BML-PW0755-0100      产品名称: Mono- and polyubiquitinated conjugates, mAb(FK2) (Biotin conjugate)   品牌: Biomol 规格: 100 ul 3周到货 生化实验

Product Specification
Clone:FK2
Host:Mouse
Isotype:IgG1
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
Source:Purified from tissue culture supernatant. Biotin conjugated chemically in vitro.
Application:Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes polyubiquitinylated and monoubiquitinylated protein conjugates in a wide range of species. Does not cross-react with free ubiquitin.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:+4°C
Long Term Storage:-20°C
Background / Technical Information:The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated (all linkage types) and monoubiquitinylated proteins but not free ubiquitin. FK2 antibody was conjugated to biotin by a proprietary method. The biotin-linked FK2 monoclonal antibody (FK2-biotin) facilitates ready detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains by Western blotting using a Streptavidin-HRP detection system.

-20°C

0823-1KG   SODIUM PHOSPHATE,MONO,MONOHYDR   品牌 Amresco

SODIUM PHOSPHATE,MONO,MONOHYDR

货号: 0823-1KG      产品名称: SODIUM PHOSPHATE,MONO,MONOHYDR   品牌: Amresco 规格: 1KG 询盘

Grade:ACS GRADE
CAS Number: 10049-21-5
Synonym(s): None
Molecular Weight: 137.99
Molecular Formula: NaH2PO4.H2O
Storage Condition: ROOM TEMPERATURE
Risk Statements: 36/37/38
Safety Statements: 36/37/39

室温

BML-PW8810-0500   Mono- and polyubiquitinylated conjugates, mAb (FK2)|正品   品牌 Biomol

Mono- and polyubiquitinylated conjugates, mAb (FK2)|正品

货号: BML-PW8810-0500      产品名称: Mono- and polyubiquitinylated conjugates, mAb (FK2)|正品   品牌: Biomol 规格: 500 ug 3周到货 生化实验

名称:Mono- and polyubiquitinylated conjugates, mAb (FK2)
货号:BML-PW8810-0500
规格:500 µg
产品说明:
Clone: FK2
Host: Mouse
Isotype: IgG1
Immunogen: Poly-ubiquitinylated lysozyme.
Application: ELISA
Immunohistochemistry
Immunoprecipitation (Care must be taken as MAb to Mono- and Polyubiquitinylated Conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilised)
Western Blot (1:1000)
Optimal conditions must be determined individually for each application.
Specificity: Recognizes mono- and polyubiquitinylated conjugates. Species independent. Does not cross-react with free ubiquitin.
Purity Detail: Purified.
Formulation: Liquid. In PBS containing 0.1% sodium azide.
Handling: Avoid freeze/thaw cycles.
Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month.  
Long Term Storage: -20°C
产品介绍:
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins but not free ubiquitin.

-20°C