pDeNy-hASC
货号： pdn-hasc 产品名称： pDeNy-hASC 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-ASC
Dominant Negative ASC gene in expression plasmid
Alias: PYCARD,CARD5
Dominant negative ASC (Pycard) (aa1-94) is a truncated form of ASC that contains the Pyrin domain but not the CARD domain. DN-ASC binds to NALP1 but not caspase-1 and strongly blocks maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1].
1. Martinon F et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hASC (pDeNy-hASC)
ORF Size: 285 bp
Subclone: AgeI – NheI
Mouse DN-mASC (pDeNy-mASC)
ORF Size: 282 bp
Subclone: NcoI – NheI
pdn-hirak pDeNy-hIRAK 品牌 Invivogen
pDeNy-hIRAK
货号： pdn-hirak 产品名称： pDeNy-hIRAK 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-IRAK
Dominant Negative IRAK gene in expression plasmid
Dominant negative IRAK (aa1-211) is a truncated form of IRAK that contains the N-terminal death domain but lacks the protein-kinase domain. This DN IRAK was shown to inhibit MyD88-induced NF-κB activation, suggesting that IRAK and MyD88 interact through their death domains [1].
1. Burns K. et al., 1998. MyD88, an adapter protein involved in interleukin-1 signaling. J Biol Chem. 273(20):12203-9.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hIRAK (pDeNy-hIRAK)
pdn-hmyd88 pDeNy-hMyD88 品牌 Invivogen
pDeNy-hMyD88
货号： pdn-hmyd88 产品名称： pDeNy-hMyD88 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-MyD88
Dominant Negative MyD88 gene in expression plasmid
Dominant negative MyD88 (aa161-296) is a truncated form of MyD88 that contains the C-terminal TIR domain but lacks the death domain. This DN MyD88 is unable to homodimerize1 and subsequently activate the signaling cascade.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hMyD88 (pDeNy-hMyD88)
Mouse DN-mMyD88 (pDeNy-mMyD88)
pdn-hnod1 pDeNy-hNOD1 品牌 Invivogen
pDeNy-hNOD1
货号： pdn-hnod1 产品名称： pDeNy-hNOD1 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-NOD1
Dominant Negative NOD1 gene in expression plasmid
Dominant negative NOD1 was generated by a deletion of the centrally located nucleotide-binding site (NBS) domain (aa 127-518) [1].
1. Bertin JJ et al. 1999. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem. 274(19):12955-8
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
human DN-hNOD1 (pDeNy-hNOD1)
pdn-hnod2 pDeNy-hNOD2 品牌 Invivogen
pDeNy-hNOD2
货号： pdn-hnod2 产品名称： pDeNy-hNOD2 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-NOD2
Dominant Negative NOD2 gene in expression plasmid
Dominant negative NOD2 was generated by a L145P mutation. This single amino-acid substitution in the CARD (caspase-recruitment domain) of the NOD2 gene inhibited the ability of the downstream factor RICK (also called RIP2 and CARDIAK) to activate NF-κB [1].
1. Tanabe T. et al. 2004. Regulatory regions and critical residues of NOD2 involved in muramyl dipeptide recognition EMBO J. 23(7): 1587-1597.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
human DN-hNOD2 (pDeNy-hNOD2)
pdn-hpkr pDeNy-hPKR 品牌 Invivogen
pDeNy-hPKR
货号： pdn-hpkr 产品名称： pDeNy-hPKR 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-PKR
Dominant Negative PKR gene in expression plasmid
Alias: EIF2AK2,PRKR
Dominant negative PKR (aa361-366) lacks six amino acids between catalytic domains IV and V which render DN-hPKR unable to autophosphorylate or activate substrate proteins. Expression of DN-hPKR in NIH 3T3 cells resulted in a malignant tranformation, suggesting that PKR may function as a suppressor of cell proliferation and tumorigenesis [1].
1. Koromilas AE et al. 1992. Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase. Science 257(5077):1685-9.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hPKR (pDeNy-hPKR)
pdn-hrigi pDeNy-hRIG-I 品牌 Invivogen
pDeNy-hRIG-I
货号： pdn-hrigi 产品名称： pDeNy-hRIG-I 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-RIG-I
Dominant Negative RIG-I gene in expression plasmid
Alias: DDX58
Dominant negative RIG-I (RIG-IC) was generated by removing the two caspase recruitment domains (CARD) found at the N terminus (aa1-217). Expression of RIG-IC in L929 cells was shown to inhibit virus-induced gene expression [1].
1. Yoneyama M. et al., 2005. Shared and Unique Functions of the DExD/H-Box Helicases RIG-I, MDA5, and LGP2 in Antiviral Innate Immunity. J. Immunol. 175:2851-58.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hRIG-I (pDeNy-hRIG-I)
Subclone: AgeI – NheI
pdn-htirap pDeNy-hTIRAP 品牌 Invivogen
pDeNy-hTIRAP
货号： pdn-htirap 产品名称： pDeNy-hTIRAP 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-TIRAP
Dominant Negative TIRAP gene in expression plasmid
Alias: MAL
Dominant negative TIRAP/Mal (P125H) was generated by mutating the proline at position 125 into a histidine. This DN TIRAP/Mal was shown to inhibit NF-κB activated by LPS-induced TLR4 but not NF-κB activated by IL-1RI or IL-18R suggesting that TIRAP/Mal is involved in TLR4 signaling [1].
1. Fitzgerald KA et al., 2001. Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction. Nature. 2001 Sep 6;413(6851):78-83.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hTIRAP (pDeNy-hTIRAP)
pdn-htraf6 pDeNy-hTRAF6 品牌 Invivogen
pDeNy-hTRAF6
货号： pdn-htraf6 产品名称： pDeNy-hTRAF6 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-TRAF6
Dominant Negative TRAF6 gene in expression plasmid
Dominant negative TRAF6 (aa289-522) harbors the C-terminal tumor necrosis factor receptor (TNFR)-associated factor (TRAF) domain but lacks the RING finger domain. Expression of this DN TRAF6 resulted in the suppression of NF-κB activity signalled by interleukin-1 (IL-1) but not by TNF [1].
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hTRAF6 (pDeNy-hTRAF6)
pdn-htram pDeNy-hTRAM 品牌 Invivogen
pDeNy-hTRAM
货号： pdn-htram 产品名称： pDeNy-hTRAM 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-TRAM
Dominant Negative TRAM gene in expression plasmid
Alias: TICAM2,TIRP
Dominant negative TRAM/TICAM2 (C117H) was generated by mutating the cysteine at position 117 into a histidine. This DN TRAM/TICAM2 was shown to retain its ability to bind to TLR4 but to lose its homo (TICAM2-TICAM2) and hetero (TICAM1-TICAM2) dimerizing properties [1].
1. Oshiumi H. et al, 2003. TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J Biol Chem. 278(50):49751-62.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hTRAM (pDeNy-hTRAM)
pdn-htrif pDeNy-hTRIF 品牌 Invivogen
pDeNy-hTRIF
货号： pdn-htrif 产品名称： pDeNy-hTRIF 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-TRIF
Dominant Negative TRIF gene in expression plasmid
Alias: TICAM1
Dominant negative TRIF (aa387-566) harbors only the TIR domain and lacks the N-terminal and C-terminal portions [1]. Furthermore, it contains a proline to histidine mutation at amino acid 434. This proline residue is conserved among TLRs and is essential for activation of the TLR-mediated
signaling.
1. Yamamoto M. et al., 2002. Cutting edge: a novel Toll/IL-1 receptor domain-containing adapter that preferentially activates the IFN-beta promoter in the Toll-like receptor signaling. J Immunol. 169(12):6668-72.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hTRIF (pDeNy-hTRIF)
pdn-masc pDeNy-mASC 品牌 Invivogen
pDeNy-mASC
货号： pdn-masc 产品名称： pDeNy-mASC 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-ASC
Dominant Negative ASC gene in expression plasmid
Alias: PYCARD,CARD5
Dominant negative ASC (Pycard) (aa1-94) is a truncated form of ASC that contains the Pyrin domain but not the CARD domain. DN-ASC binds to NALP1 but not caspase-1 and strongly blocks maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1].
1. Martinon F et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hASC (pDeNy-hASC)
ORF Size: 285 bp
Subclone: AgeI – NheI
Mouse DN-mASC (pDeNy-mASC)
ORF Size: 282 bp
Subclone: NcoI – NheI
pdn-mcs pDeNy-mcs 品牌 Invivogen
pDeNy-mcs
货号： pdn-mcs 产品名称： pDeNy-mcs 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-mcs
pDeNy is a cloning plasmid for the expression of dominant negative (DN) forms of the Toll-Like Receptor (TLR) signaling genes.
Since pDeNy-mcs does not contain a DN TLR signaling gene it can be used in conjunction with other vectors of the pDeNy family to serve as an experimental control.
– Cotransfectable with pUNO plasmids
– pDeNy plasmids can be used for transient or stable transfection experiments
– Selectable with Zeocin™ in both E. coli and mammalian cells
pdn-mmyd88 pDeNy-mMyD88 品牌 Invivogen
pDeNy-mMyD88
货号： pdn-mmyd88 产品名称： pDeNy-mMyD88 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-MyD88
Dominant Negative MyD88 gene in expression plasmid
Dominant negative MyD88 (aa161-296) is a truncated form of MyD88 that contains the C-terminal TIR domain but lacks the death domain. This DN MyD88 is unable to homodimerize1 and subsequently activate the signaling cascade.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hMyD88 (pDeNy-hMyD88)
Mouse DN-mMyD88 (pDeNy-mMyD88)