psiRNA-hMyD88 Kit
货号： ksirna42-hmyd88 产品名称： psiRNA-hMyD88 Kit 品牌： Invivogen 规格： 1 kit 三周到货 生化试验
psiRNA-MyD88
psiRNA vector expressing shRNA targeting human MyD88 gene
Name: MyD88
Genbank: NM_002468
Backbone: psiRNA-h7SK GFPzeo
Selection: Zeocin™
psiRNA vector expressing shRNA targeting mouse MyD88 gene
Name: MyD88
Genbank: NM_010851
Backbone: psiRNA-h7SK GFPzeo
Selection: Zeocin™
pdn-hmyd88 pDeNy-hMyD88 品牌 Invivogen
pDeNy-hMyD88
货号： pdn-hmyd88 产品名称： pDeNy-hMyD88 品牌： Invivogen 规格： 1 disk 二周到货 生化试验
pDeNy-MyD88
Dominant Negative MyD88 gene in expression plasmid
Dominant negative MyD88 (aa161-296) is a truncated form of MyD88 that contains the C-terminal TIR domain but lacks the death domain. This DN MyD88 is unable to homodimerize1 and subsequently activate the signaling cascade.
This gene is available in pDeNy expression plasmid selectable with Zeocin™.
Contents update notification
All InvivoGen plasmids are now provided as lyophilized DNA.
Human DN-hMyD88 (pDeNy-hMyD88)
Mouse DN-mMyD88 (pDeNy-mMyD88)
psirna42-hmyd88 psiRNA-hMyD88 品牌 Invivogen
psiRNA-hMyD88
货号： psirna42-hmyd88 产品名称： psiRNA-hMyD88 品牌： Invivogen 规格： 20 μg 三周到货 生化试验
psiRNA-MyD88
psiRNA vector expressing shRNA targeting human MyD88 gene
Name: MyD88
Genbank: NM_002468
Backbone: psiRNA-h7SK GFPzeo
Selection: Zeocin™
psiRNA vector expressing shRNA targeting mouse MyD88 gene
Name: MyD88
Genbank: NM_010851
Backbone: psiRNA-h7SK GFPzeo
Selection: Zeocin™
punoha-hmyd88 pUNO-hMyD88-HA 品牌 Invivogen
pUNO-hMyD88-HA
货号： punoha-hmyd88 产品名称： pUNO-hMyD88-HA 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
MyD88-HA
Coding sequence in expression plasmid
Myeloid Differentiation primary response gene 88, HA-tagged
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin or in pUNO2-HA expression plasmid selectable with Zeocin™.
Human MyD88-HA (pUNO1-hMyD88-HA3x)
Mouse MyD88-HA (pUNO2-mMyD88-HA3x)
puno-hmyd88 pUNO-hMyD88 品牌 Invivogen
pUNO-hMyD88
货号： puno-hmyd88 产品名称： pUNO-hMyD88 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
MyD88
Coding sequence in expression plasmid
Myeloid Differentiation Primary Response 88
Myeloid differentiation factor 88 (MyD88) is a cytoplasmic adaptor protein that is involved in interleukin-1 receptor (IL-1R)- and Toll-like receptor (TLR)-induced activation of NF-kappaB. MyD88 contains a C-terminal Toll/IL-1R homology (TIR) domain and an N-terminal death domain (DD), which mediate the interaction of MyD88 with IL-1R/TLR and IRAK, respectively [1]. MyD88 is essential for signaling as MyD88 -/- mice fail to induce cytokines in response to LPS and CpG DNA [2]. Some TLRs induce antiviral responses by producing IFN-alpha in a MyD88-dependent manner. MyD88 interacts with IRF7 resulting in the activation of IFN-alpha-dependent promoters [3].
1. Janssens S. et al., 2002. Regulation of interleukin-1- and lipopolysaccharide-induced NF-kappaB activation by alternative splicing of MyD88. Curr Biol. 12(6):467-71.
2. Kawai T. et al. 1999. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 11(1):115-22.
3. Kawai T. et al., 2004. Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6. Nat Immunol. 5(10):1061-8.
This gene is available in pUNO1 expression plasmid selectable with Blasticidin.
Human MyD88 (pUNO1-hMYD88)
Genbank: NM_002468.4
ORF Size: 891 bp
Subclone: NcoI – NheI
Mouse MyD88 (pUNO1-mMYD88)
ORF Size: 891 bp
Subclone: BspLU11I – NheI