A-MONOMETHYL-HIST H3 (LYS9)100UL

货号： 07-395      产品名称： A-MONOMETHYL-HIST H3 (LYS9)100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-monomethyl-Histone H3 (Lys9) Antibody
Close
REFERENCES
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins.
Schultz, David C, et al. (2002) Genes Dev., 16: 919-32 (2002)
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Nakayama, J, et al. (2001) Science, 292: 110-3 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Serum Polyclonal Antibody
Description:
Anti-monomethyl-Histone H3 (Lys9) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
methylated Histone H3 (Lys9)
Molecular Weight:
17 kDa
Immunogen:
synthetic peptide containing the sequence ARmeKS corresponding to monomethyl-lysine at residue 9 of human Histone H3.
Modifications:
Methylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot in acid-extracted proteins from HeLa cells
Purification Method:
Antiserum
Presentation:
0.05% sodium azide and 30% glycerol
Storage Conditions:
1 year at -20°C
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
HIST3H3
H3FT
MGC126886
H3t
MGC126888
H3T
H3/g
H3.4
H3/t
View All »
Alternate Names:
H3K9me1
Histone H3 (mono methyl K9)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S -20℃

A-MONOMETHYL-HIST H3 (LYS4) 200UG

货号： 07-436      产品名称： A-MONOMETHYL-HIST H3 (LYS4) 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-monomethyl-Histone H3 (Lys4) Antibody
Close
REFERENCES
DNA Replication Origin Function Is Promoted by H3K4 Di-methylation in Saccharomyces cerevisiae.
Lindsay F Rizzardi,Elizabeth S Dorn,Brian D Strahl,Jeanette Gowen Cook (2012) Genetics.192
Molecular mechanisms controlling CFTR gene expression in the airway.
Zhaolin Zhang,Christopher J Ott,Marzena A Lewandowska,Shih-Hsing Leir,Ann Harris (2012) Journal of cellular and molecular medicine.16
Lysyl oxidase-like 2 deaminates lysine 4 in histone H3.
Herranz, Nicolás, et al. (2012) Mol. Cell, 46: 369-76 (2012)
Molecular mechanisms controlling CFTR gene expression in the airway.
Zhang Z, Ott CJ, Lewandowska MA, Leir SH, Harris A (2011) Journal of cellular and molecular medicine
View All
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt  ICC, IF, PIA, WB, ChIP-seq, DB Rabbit Purified Polyclonal Antibody
Description:
Anti-monomethyl-Histone H3 (Lys4) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes monomethyl-histone H3 (Lys4), Mr 17kDa.
Molecular Weight:
17 kDa
Epitope:
Lys4
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence ART[meK]…, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species to cross-reactivity is expected.
Application Notes:
Immunocytochemistry:
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
View All »
Control:
HeLa cell extract
Quality Assurance:
Routinely evaluated by Western Blot in acid extracted proteins from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
View All »
Purification Method:
Protein A purfied
Presentation:
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2 to 8°C from date of receipt.
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
HIST3H3
H3FT
H3/t
H3t
MGC126888
H3/g
H3.4
H3T
MGC126886
View All »
Alternate Names:
H3K4me1
Histone H3 (mono methyl K4)
H3 histone family, member T
histone 3, H3
histone cluster 3, H3
View All »
Usage Statement:
Un 2-8℃

A-MONOMETHYL-HIST H3 (LYS27) 200UG

货号： 07-448      产品名称： A-MONOMETHYL-HIST H3 (LYS27) 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-monomethyl-Histone H3 (Lys27) Antibody
Close
REFERENCES
Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila.
Hans-Martin Herz,Man Mohan,Alexander S Garrett,Caitlynn Miller,David Casto,Ying Zhang,Christopher Seidel,Jeffrey S Haug,Laurence Florens,Michael P Washburn,Masamitsu Yamaguchi,Ramin Shiekhattar,Ali Shilatifard (2012) Molecular and cellular biology.32
The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
Vandamme, Julien, et al. (2012) PLoS Genet., 8: e1002647 (2012)
Pairing of lacO tandem repeats in Arabidopsis thaliana nuclei requires the presence of hypermethylated, large arrays at two chromosomal positions, but does not depend on H3-lysine-9-dimethylation.
Gabriele Jovtchev,Branimira Emilova Borisova,Markus Kuhlmann,Jörg Fuchs,Koichi Watanabe,Ingo Schubert,Michael Florian Mette (2011) Chromosoma.120
Broad chromosomal domains of histone modification patterns in C. elegans.
Liu T, Rechtsteiner A, Egelhofer TA, Vielle A, Latorre I, Cheung MS, Ercan S, Ikegami K, Jensen M, Kolasinska-Zwierz P, Rosenbaum H, Shin H, Taing S, Takasaki T, Iniguez AL, Desai A, Dernburg AF, Kimura H, Lieb JD, Ahringer J, Strome S, Liu XS (2011) Genome Res.21:227-36. Epub 2010 Dec 22.
View All
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt  DB, ICC, WB, ChIP-seq Rabbit Purified Polyclonal Antibody
Description:
Anti-monomethyl-Histone H3 (Lys27) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.
Molecular Weight:
17 kDa
Epitope:
Lys27
Immunogen:
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity expected, based on sequence homology.
Application Notes:
Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Control:
HeLa acid extract
Quality Assurance:
Routinely evaluated by western blot acid in extracted proteins from Hela cells.
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.
View All »
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q16695
Entrez Gene Number:
NP_003484
Gene Symbol:
HIST3H3
H3FT
H3/t 2-8℃

A-MONOMETHYL-HIST H3 (LYS27)Rb Mg

货号： 07-448-K      产品名称： A-MONOMETHYL-HIST H3 (LYS27)Rb Mg   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-monomethyl-Histone H3 (Lys27) Antibody
Close
REFERENCES
Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila.
Hans-Martin Herz,Man Mohan,Alexander S Garrett,Caitlynn Miller,David Casto,Ying Zhang,Christopher Seidel,Jeffrey S Haug,Laurence Florens,Michael P Washburn,Masamitsu Yamaguchi,Ramin Shiekhattar,Ali Shilatifard (2012) Molecular and cellular biology.32
The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
Vandamme, Julien, et al. (2012) PLoS Genet., 8: e1002647 (2012)
Pairing of lacO tandem repeats in Arabidopsis thaliana nuclei requires the presence of hypermethylated, large arrays at two chromosomal positions, but does not depend on H3-lysine-9-dimethylation.
Gabriele Jovtchev,Branimira Emilova Borisova,Markus Kuhlmann,Jörg Fuchs,Koichi Watanabe,Ingo Schubert,Michael Florian Mette (2011) Chromosoma.120
Broad chromosomal domains of histone modification patterns in C. elegans.
Liu T, Rechtsteiner A, Egelhofer TA, Vielle A, Latorre I, Cheung MS, Ercan S, Ikegami K, Jensen M, Kolasinska-Zwierz P, Rosenbaum H, Shin H, Taing S, Takasaki T, Iniguez AL, Desai A, Dernburg AF, Kimura H, Lieb JD, Ahringer J, Strome S, Liu XS (2011) Genome Res.21:227-36. Epub 2010 Dec 22.
View All
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt  DB, ICC, WB, ChIP-seq Rabbit Purified Polyclonal Antibody
Description:
Anti-monomethyl-Histone H3 (Lys27) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.
Molecular Weight:
17 kDa
Epitope:
Lys27
Immunogen:
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity expected, based on sequence homology.
Application Notes:
Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Control:
HeLa acid extract
Quality Assurance:
Routinely evaluated by western blot acid in extracted proteins from Hela cells.
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.
View All »
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q16695
Entrez Gene Number:
NP_003484
Gene Symbol:
HIST3H3
H3FT
H3/t 2-8℃

A-MONOMETHYL-HIST H3 (LYS9) 100UG

货号： 07-450      产品名称： A-MONOMETHYL-HIST H3 (LYS9) 100UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-monomethyl-Histone H3 (Lys9) Antibody
Close
REFERENCES
Impairment of prostate cancer cell growth by a selective and reversible lysine-specific demethylase 1 inhibitor.
Dominica Willmann,Soyoung Lim,Stefan Wetzel,Eric Metzger,Anett Jandausch,Wolfram Wilk,Manfred Jung,Ignasi Forne,Axel Imhof,Andreas Janzer,Jutta Kirfel,Herbert Waldmann,Roland Sch,Reinhard Buettner (2012) International journal of cancer. Journal international du cancer.131
Sequential establishment of marks on soluble histones H3 and H4.
Francisca Alvarez,Francisca Muñoz,Pierre Schilcher,Axel Imhof,Geneviève Almouzni,Alejandra Loyola (2011) The Journal of biological chemistry.286
Migration cues induce chromatin alterations.
Gabi Gerlitz,Idit Livnat,Carmit Ziv,Oded Yarden,Michael Bustin,Orly Reiner (2007) Traffic (Copenhagen, Denmark).8
Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation.
Michael Schnekenburger,Glenn Talaska,Alvaro Puga (2007) Molecular and cellular biology.27
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, Ch  DB, ICC, PIA, WB Rabbit Purified Polyclonal Antibody
Description:
Anti-monomethyl-Histone H3 (Lys9) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes monomethylated histone H3 (Lys9), Mr ~17 kDa.
Molecular Weight:
17 kDa
Epitope:
Lys9
Immunogen:
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKST… in which meK corresponds to monomethyl-lysine at residue 9 of human histone H3.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Mouse
Chicken
Species Reactivity Note:
Human, mouse and chicken. Broad species cross-reactivity is expected based on sequence homology.
Application Notes:
Peptide Competition:
Specificity confirmed by the ability of 10 μM of the immunizing peptide to abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts (Figure B, Lane 2). No signal reductions were detected with preincubation with 10 μM di- or trimethyl-lysine 9 or di- or trimethyl-lysine 27 modified peptides. Some signal reduction detected after preincubation with 10 μM monomethyl-lysine 27 modified peptide (Figure B, Lane 5, page 2).
Immunocytochemistry & Dot Blot: Reported by an independent laboratory1-4.
View All »
Control:
HeLa cell extract
Quality Assurance:
Routinely evaluated by Western Blot on acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3.
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
View All »
Purification Method:
Protein A purfied
Present -20℃

a-monomethyl-Hist H4 (Lys20) 200ug

货号： 07-748      产品名称： a-monomethyl-Hist H4 (Lys20) 200ug   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-monomethyl-Histone H4 (Lys20) Antibody
Close
PRODUCT FAMILY INFORMATION
Histone H4 Antibodies and Proteins
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon.
EMD Millipore’s Histone H4 antibody demonstrates specificity against linker histone H4. See below for data and references for EMD Millipore’s Histone H4 antibodies and proteins. All EMD Millipore antibodies and proteins are based on the expertise of Upstate & Chemicon.
» More…
Species Reactivity Key Applications Host Format Antibody Type
H  WB, Mplex, ICC Rabbit Purified Polyclonal Antibody
Description:
Anti-monomethyl-Histone H4 (Lys20) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
monomethylated Histone H4 (Lys20)
Molecular Weight:
10kDa
Immunogen:
KLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[me1K]VLRDN) of human Histone H4
Modifications:
Methylation
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Broad species cross-reactivity expected due to sequence conservation
Application Notes:
Additional Referenced Research Applications:
Immunocytochemistry (cells)
Quality Assurance:
Routinely evaluated by immunoblot
Purification Method:
Protein A purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
Q6DRA9
P62805
Entrez Gene Number:
NM_001034077.4
Gene Symbol:
HIST2H4B
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
H4/o
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
HIST1H4E
HIST4H4
HIST2H4A
H4FC
H4FI
H4/H
HIST1H4C
H4/G
HIST1H4K
H4FJ
H4FD
H4/I
H4/B
H4/C
H4/D
HIST1H4J
HIST1H4D
H4/E
View All »
Alternate Names:
H4K20me1
Histone H4 (mono methyl K20)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Multiplexing
Immunocytochemistry
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
View All »
UniProt Summary:

Monomethyl-Hist H3 (Lys4) Pept, biotin 1

货号： 12-563      产品名称： Monomethyl-Hist H3 (Lys4) Pept, biotin 1   品牌： Millipore 规格： EA 三周到货 生化实验

Monomethyl-Histone H3 (Lys4) Peptide, biotin conjugate
Close
PRODUCT FAMILY INFORMATION
Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
» More…
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
» More…
Description:
Monomethyl-Histone H3 (Lys4) Peptide, biotin conjugate
Trade Name:
Upstate (Millipore)
Qty/Pk:
100 µg
Applications:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Key Applications:
Immunoprecipitation
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Me -20℃