A-MONOMETHYL-HIST H3 (LYS27) 200UG

货号： 07-448      产品名称： A-MONOMETHYL-HIST H3 (LYS27) 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-monomethyl-Histone H3 (Lys27) Antibody
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REFERENCES
Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila.
Hans-Martin Herz,Man Mohan,Alexander S Garrett,Caitlynn Miller,David Casto,Ying Zhang,Christopher Seidel,Jeffrey S Haug,Laurence Florens,Michael P Washburn,Masamitsu Yamaguchi,Ramin Shiekhattar,Ali Shilatifard (2012) Molecular and cellular biology.32
The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
Vandamme, Julien, et al. (2012) PLoS Genet., 8: e1002647 (2012)
Pairing of lacO tandem repeats in Arabidopsis thaliana nuclei requires the presence of hypermethylated, large arrays at two chromosomal positions, but does not depend on H3-lysine-9-dimethylation.
Gabriele Jovtchev,Branimira Emilova Borisova,Markus Kuhlmann,Jörg Fuchs,Koichi Watanabe,Ingo Schubert,Michael Florian Mette (2011) Chromosoma.120
Broad chromosomal domains of histone modification patterns in C. elegans.
Liu T, Rechtsteiner A, Egelhofer TA, Vielle A, Latorre I, Cheung MS, Ercan S, Ikegami K, Jensen M, Kolasinska-Zwierz P, Rosenbaum H, Shin H, Taing S, Takasaki T, Iniguez AL, Desai A, Dernburg AF, Kimura H, Lieb JD, Ahringer J, Strome S, Liu XS (2011) Genome Res.21:227-36. Epub 2010 Dec 22.
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt  DB, ICC, WB, ChIP-seq Rabbit Purified Polyclonal Antibody
Description:
Anti-monomethyl-Histone H3 (Lys27) Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.
Molecular Weight:
17 kDa
Epitope:
Lys27
Immunogen:
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity expected, based on sequence homology.
Application Notes:
Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Control:
HeLa acid extract
Quality Assurance:
Routinely evaluated by western blot acid in extracted proteins from Hela cells.
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.
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Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q16695
Entrez Gene Number:
NP_003484
Gene Symbol:
HIST3H3
H3FT
H3/t 2-8℃