标签归档：ip

4277-10   CXCL10/IP-10/CRG-2, human recombinant   品牌 Biovision

发表于由

CXCL10/IP-10/CRG-2, human recombinant

货号： 4277-10      产品名称： CXCL10/IP-10/CRG-2, human recombinant   品牌： Biovision 规格： 10 μg 3周到货 生化实验

CXCL10/IP-10/CRG-2, human recombinant
Chemoattractant for monocytes, T cells, NK cells, and dendritic cells
Product Overview
Product Name: CXCL10/IP-10/CRG-2, human recombinant
Alternate Name/Synonyms: C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) Cleaved into: CXCL10(1-73)
Gene Symbol: CXCL10
Accession #: P02778
Gene ID: 3627
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 150 mM NaCl, pH 7.0.
Molecular Weight: 8.8 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg (1 IEU/μg)
Biological Activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 μg/mL.
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/ml). This can further be diluted to other aqueous buffers.
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Human Chemokine (C-X-C motif) ligand 10(CXCL10),also known as Interferon gamma-inducible protein-10 (IP-10), is a non-ELR chemokine secreted by various cell types including monocytes, endothelial cells and fibroblasts in response to IFN-γ. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles, such as chemo-attraction for activated T-lymphocytes, inhibition of angiogenesis and antitumor activity.
Amino Acid Sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

4277-50   CXCL10/IP-10/CRG-2, human recombinant   品牌 Biovision

发表于由

CXCL10/IP-10/CRG-2, human recombinant

货号： 4277-50      产品名称： CXCL10/IP-10/CRG-2, human recombinant   品牌： Biovision 规格： 50 μg 3周到货 生化实验

CXCL10/IP-10/CRG-2, human recombinant
Chemoattractant for monocytes, T cells, NK cells, and dendritic cells
Product Overview
Product Name: CXCL10/IP-10/CRG-2, human recombinant
Alternate Name/Synonyms: C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) Cleaved into: CXCL10(1-73)
Gene Symbol: CXCL10
Accession #: P02778
Gene ID: 3627
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 150 mM NaCl, pH 7.0.
Molecular Weight: 8.8 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg (1 IEU/μg)
Biological Activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 μg/mL.
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/ml). This can further be diluted to other aqueous buffers.
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Human Chemokine (C-X-C motif) ligand 10(CXCL10),also known as Interferon gamma-inducible protein-10 (IP-10), is a non-ELR chemokine secreted by various cell types including monocytes, endothelial cells and fibroblasts in response to IFN-γ. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles, such as chemo-attraction for activated T-lymphocytes, inhibition of angiogenesis and antitumor activity.
Amino Acid Sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

4277-1000   CXCL10/IP-10/CRG-2, human recombinant   品牌 Biovision

发表于由

CXCL10/IP-10/CRG-2, human recombinant

货号： 4277-1000      产品名称： CXCL10/IP-10/CRG-2, human recombinant   品牌： Biovision 规格： 1 mg 3周到货 生化实验

CXCL10/IP-10/CRG-2, human recombinant
Chemoattractant for monocytes, T cells, NK cells, and dendritic cells
Product Overview
Product Name: CXCL10/IP-10/CRG-2, human recombinant
Alternate Name/Synonyms: C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) Cleaved into: CXCL10(1-73)
Gene Symbol: CXCL10
Accession #: P02778
Gene ID: 3627
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 150 mM NaCl, pH 7.0.
Molecular Weight: 8.8 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg (1 IEU/μg)
Biological Activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 μg/mL.
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/ml). This can further be diluted to other aqueous buffers.
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Human Chemokine (C-X-C motif) ligand 10(CXCL10),also known as Interferon gamma-inducible protein-10 (IP-10), is a non-ELR chemokine secreted by various cell types including monocytes, endothelial cells and fibroblasts in response to IFN-γ. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles, such as chemo-attraction for activated T-lymphocytes, inhibition of angiogenesis and antitumor activity.
Amino Acid Sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

BML-SA254-0100   IP3 receptor type 1, pAb   品牌 Biomol

发表于由

IP3 receptor type 1, pAb

货号： BML-SA254-0100      产品名称： IP3 receptor type 1, pAb   品牌： Biomol 规格： 100 ul 3周到货 生化实验

IP3 receptor type 1, pAb
Product Specification
Alternative Name:IP3R1, ITPR1, IP3R-I, Inositol 1,4,5-triphosphate receptor type 1, INSP3R1
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 1829-1848 of human IP3R1.
UniProt ID:Q14643
Application:Immunohistochemistry
Western Blot
Specificity:Recognizes human, rat and dog IP3R1. Detects a band of ~240kDa by Western blot.
Formulation:Liquid. Neat serum containing 0.05% sodium azide.
Long Term Storage:-20°C

-20°C

23625   |Mammalian c-Myc Tag IP/Co-IP Kit|哺乳动物c-Myc-Tag IP/Co-IP   品牌 Pierce

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|Mammalian c-Myc Tag IP/Co-IP Kit|哺乳动物c-Myc-Tag IP/Co-IP

货号： 23625      产品名称： |Mammalian c-Myc Tag IP/Co-IP Kit|哺乳动物c-Myc-Tag IP/Co-IP   品牌： Pierce 规格： KIT

Pierce c-Myc Tag IP/Co-IP Kit     
Perform IP or Co-IP experiments with a c-Myc tagged protein.
The Thermo Scientific Pierce c-Myc Tag IP/Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments using a c-Myc-tagged protein as the "bait".
The c-Myc peptide (EQKLISEEDL) has become a popular fusion tag for mammalian recombinant protein expression. This kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized. Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged "bait" protein are captured on the agarose beads. After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis. The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions.
Highlights:
Specific – the immobilized anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection (Figures 1 and 2)
High Capacity – excellent results with as little as 1 µg of anti-c-Myc antibody in IP mode with the positive control lysate
Rigorous – kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls (see Figure 2 for example controls)
Robust – the affinity system is compatible with IP or Co-IP from various cell lysates and physiological (non-denaturing) buffer systems; choose the Mammalian Kit to use the validated M-PER Mammalian Cell Lysis Reagent for the procedure
Convenient and Easy – complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions 

26146   |Pierce Classic IP Kit/经典的免疫沉淀试剂盒   品牌 Pierce

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|Pierce Classic IP Kit/经典的免疫沉淀试剂盒

货号： 26146      产品名称： |Pierce Classic IP Kit/经典的免疫沉淀试剂盒   品牌： Pierce 规格： KIT 现货

Pierce Classic IP Kit/经典的免疫沉淀试剂盒
用于快速有效地回收免疫复合
Thermo Scientific 经典免疫沉淀试剂盒使用微型离心机离心柱和收集管可轻松实现蛋白A/G琼脂糖树脂与回收蛋白的分离。不需进行冗长的吸液操作将上清与树脂分离。简单地加入含有与蛋白A/G树脂结合的免疫复合物的样品并离心即可除去未结合的物质。加入洗脱缓冲液并离心可回收免疫沉淀蛋白。或者可以加入试剂盒提供的样品上样缓冲液（loadingbuffer）并煮沸。
产品特点：
改进的实验一致性-离心柱可去除树脂损失，改善样品回收率并保证与树脂有效地分离
易于操作-只需简单的操作即可从裂解液粗提物中免疫沉淀靶蛋白
快速-在不到一小时内即可完成整个实验
经济-可在今后的免疫沉淀实验中重复使用蛋白A/G琼脂糖树脂
完全-试剂盒包含足够进行至少50次IP实验的成分

 

26147   |Pierce Crosslink IP Kit/交联免疫沉淀试剂盒   品牌 Pierce

发表于由

|Pierce Crosslink IP Kit/交联免疫沉淀试剂盒

货号： 26147      产品名称： |Pierce Crosslink IP Kit/交联免疫沉淀试剂盒   品牌： Pierce 规格： KIT 现货

Pierce Crosslink IP Kit/交联免疫沉淀试剂盒
可回收更多蛋白质且无抗体干扰
产品特点：
无抗体污染-没有抗体的重链和轻链被洗脱下来，因此不会对纯化蛋白的SDS-PAGE分析造成干扰
改进的实验可信度和样品处理方式-离心柱可消除树脂的损失，并且与传统免疫沉淀相比能更有效地分离溶液
完全-试剂盒包含足够固相化多种抗体并进行至少50次IP实验的成分
Thermo Scientific 交联免疫沉淀试剂盒通过加入交联技术和微型离心机离心柱样品处理步骤，扩展了传统免疫沉淀法的功能。这些特点的主要好处是纯化靶蛋白而无抗体污染，并可将样品从珠状琼脂糖树脂上高效洗涤和分离下来。
 

交联免疫沉淀试剂盒 货号 名称 包装 26147 交联免疫沉淀试剂盒
足够用于至少50次免疫沉淀反应的试剂
包括：蛋白A/G Plus琼脂糖
对照用琼脂糖树脂
DSS交联剂
偶联缓冲液（20×）
免疫沉淀裂解/洗涤缓冲液
条件缓冲液（conditioning buffer0）（100×）
Tris缓冲盐溶液（25×）
洗脱缓冲液
上样缓冲液（5×）
离心柱和收集管 试剂盒
 
1ml
2ml
8×2mg
25ml
2×50ml
5ml
25ml
50ml
5ml