标签归档：irak

V2621   IRAK4 Kinase Enzyme System   品牌 Promega

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IRAK4 Kinase Enzyme System

货号： V2621      产品名称： IRAK4 Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在 Sf9 昆虫细胞中，我们通过杆状病毒 表达 N 端带 GST 标签的 重组 全长人源 IRAK4 。 IRAK4 （ Interleukin-1 receptor-associated kinase 4 ，白细胞介素 1 受体相关的激酶 4 ）在介导 TLR （ Toll-like receptor ， Toll 样受体）与 IL1R （ IL-1 receptor ，白细胞介素 1 受体）家族成员的信号转导过程中发挥着重要作用。 IRAK4 参与了引起细胞凋亡的 TLR 信号转导过程；此外，它还具有结合蛋白质、结合 ATP 、结合镁离子、以及激酶活性等分子功能。在许多物种中， Toll/IL1R 受体家族成员像 IRAK4 一样，是其宿主防御机制的中心组分。在其信号转导过程中，丝 / 苏氨酸激酶活性非常保守，能将质膜上受体复合物的早期信号事件与胞质中的较大 信号 复合体偶联。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： IRAK4 ， 10μg ( 人源，重组全长蛋白 ) ； MW : ~ 81 kDa 。
•  底物： 天然的猪髓磷脂碱性 蛋白（ MBP ） 。
•  其它组分： 反应缓冲液， DTT 。
IRAK4 NCBI 数据库入口。
人类激酶酶系统及其应用。 
特点  •  在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2 。
•  可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 

04-368   anti-IRAK-4 (N-term)   品牌 Millipore

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anti-IRAK-4 (N-term)

货号： 04-368      产品名称： anti-IRAK-4 (N-term)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-IRAK-4 (N-term), clone Y279, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC Rabbit Unpurified Monoclonal Antibody
Description:
Anti-IRAK-4 (NT) Antibody, clone Y279, rabbit monoclonal
Promotional Text:
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Specificity:
This antibody recognizes the N-terminus of IRAK-4.
Molecular Weight:
~52 kDa observed
Epitope:
N-terminus
Immunogen:
Synthetic peptide corresponding to the N-terminus of human IRAK-4.
Clone:
Y279
Isotype:
IgG
Background Information:
IRAK-4 is a member of the interleukin-1 receptor-associated kinase (IRAK) family. The IRAK family is implicated in the Toll-like receptor (TLR) and Il-1R signaling pathway (1). Upon phosphorylation, IRAK-4 interacts with IRAK-1, MYD88 and TRAF6 to form a complex, and activate NF-kB and MAPK pathways (2-3). IRAK-4 phosphorylates and activates IRAK-1 at thr387 and is also known to interact with IRAK-1 but not with the other IRAK members. Additionally, like IRAK-1, IRAK-4 possesses an auto and crossphosphorylation kinase activity (4).
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Immunocytochemistry Analysis: A 1:50-100 dilution from a representative lot was used in IC.
Control:
Daudi cell lysate
Quality Assurance:
Evaluated by Western Blot in Jurkat cell lysate
WesternBlot Analysis: A 1:1,000 to 1:10,000 dilution of this antibody detected IRAK-4 in Jurkat cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q9NWZ3
Entrez Gene Number:
Q9NWZ3
Gene Symbol:
NY-REN-64
IRAK-4
EC 2.7.11.1
IPD1
REN64
EC 2.7.11
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Alternate Names:
interleukin-1 receptor-associated kinase 4
Renal carcinoma antigen NY-REN-64
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Product Name:
Anti-IRAK-4 (N-term), clone Y279, Rabbit Monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Unpurified
Host:
Rabbit
Applications:
Anti-IRAK-4 (N-term) Antibody, clone Y279, Rabbit Monoclonal is an antibody against IRAK-4 (N-term) for use in Western Blotting, ICC.

-20°C