标签归档：erk

V1961   ERK2 Kinase Enzyme System   品牌 Promega

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ERK2 Kinase Enzyme System

货号： V1961      产品名称： ERK2 Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 我们在大肠杆菌（E. coli ）细胞中 表达 N 末端带 GST 标签的 重组 全长人源 ERK2 ，该蛋白在体外可被 MEK1 激活。 ERK2 是一个丝/ 苏氨酸蛋白激酶，属于胞外信号调控激酶（ERKs）的成员，被许多生长因子和细胞因子激活；其激活需要 MEK 介导的酪氨酸和苏氨酸的磷酸化。 ERK2 广泛分布于许多组织中，在心脏、大脑和脊髓中表达量最高。激活的 ERK2 转运到细胞核内，磷酸化多种转录因子（如Elk-1 、
c-Myc 、c-Jun 、c-Fos 以及 C/EBP β）。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： ERK2 ， 10μg ( 人源，重组全长蛋白 ) ； MW : ~ 68 kDa 。
•  底物： 天然的猪髓磷脂碱性 蛋白（ MBP ） 。
•  其它组分： 反应缓冲液， DTT 。
ERK2 NCBI 数据库入口。
人类激酶酶系统及其应用。 
特点  •  在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2 。
•  可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 

7741-5   ERK1, Active   品牌 Biovision

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ERK1, Active

货号： 7741-5      产品名称： ERK1, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active ERK1
Involved in regulation of meiosis, mitosis, and postmitotic functions
Product Overview
Alternate Names / Synonyms: erk-1, erk 1, Active ERK1, ERK1, ERK, human ERK1, active kinase, active kinases, recombinant ERK1, recombinant ERK, kinase activity assay, protein kinase
Gene Symbol: ERK1
Accession #: P27361
Gene ID: 5595
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 45.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 383 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs), which are activated in response to numerous growth factors and cytokines. Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. In vitro studies indicate that ERK1 phosphorylate both nuclear and cytoplasmic proteins. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP β). The consensus primary sequence for substrate phosphorylation by ERK1 has been identified as -Pro-Leu-Ser/Thr-Pro-. ERK1 has been implicated in the control of a broad spectrum of cellular events in many types of cells. In somatic cells, ERK1 activation seems to be triggered after exit from a quiescent state (in G0 or G2) only and then inactivated by entry into a proliferative state.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

BML-MA1366-0025   ERK1/ERK2 (phosphorylated), mAb (AMK1)   品牌 Biomol

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ERK1/ERK2 (phosphorylated), mAb (AMK1)

货号： BML-MA1366-0025      产品名称： ERK1/ERK2 (phosphorylated), mAb (AMK1)   品牌： Biomol 规格： 25 ug 3周到货 生化实验

Product Specification
Alternative Name:MAPK2/3, MAP kinase 2/3
Clone:AMK1
Host:Mouse
Isotype:IgG1
Immunogen:Synthetic peptide containing the motif pThr-Glu-pTyr.
Application:Western Blot
Specificity:Recognizes the pThr-Glu-pTyr motif of activated mammalian ERK1/2. Does not cross-react with non-activated MAP kinases or with the phosphorylated motifs of SAPK/JNK (Thr-Pro-Tyr) or p38 kinases (Thr-Gly-Tyr).
Formulation:Lyophilized.
Long Term Storage:-20°C

-20°C

BML-P124-0001   ERK1/ERK2 Peptide   品牌 Biomol

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ERK1/ERK2 Peptide

货号： BML-P124-0001      产品名称： ERK1/ERK2 Peptide   品牌： Biomol 规格： 1 mg 3周到货 生化实验

This sequence, ADPDHDHTGFLTGYVATRTRR, is based on Erk1/Erk2 residues 172-190, containing a MAP kinase kinase phosphorylation site (underlined). N-terminal Arg is added for phosphocellulose binding.
Product Specification
Sequence:Ala-Asp-Pro-Asp-His-Asp-His-Thr-Gly-Phe-Leu-Thr-Glu-Tyr-Val-Ala-Thr-Arg-Trp-Arg-Arg
MW:2543.8
Purity:97%
Long Term Storage:-20°C

-20°C

328010-500UG   InSolution ERK Inhibitor II, FR180204   品牌 Merck

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InSolution ERK Inhibitor II, FR180204

货号： 328010-500UG      产品名称： InSolution ERK Inhibitor II, FR180204   品牌： Merck 规格： *** 3周到货 生化实验

InSolution™ ERK Inhibitor II, FR180204
A cell-permeable pyrazolopyridazinamine that acts as a potent, ATP-competitive inhibitor of ERK1 and ERK2 (IC50 = 510 nM and 330 nM; Ki = 310 nM and 140 nM, respectively). Exhibits ~20-fold greater selectivity over p38α (IC50 = 10 µM) and shows little activity towards IKKα, MEK1, MKK4, PDGFRα, PKCα, Src, and Syk even at concentrations as high as 30 µM. Shown to inhibit TGF-β−induced AP-1 activation in Mv1Lu epithelial cells (IC50 of 3.1 µM). ERK Inhibitor II, Negative Control (Cat. No. 328008) and the solid form of this compound (Cat. No. 328007) are also available..
产品信息
Form  Liquid
Formulation  A 10 mM (500 µg/153 µl) solution of ERK Inhibitor II, FR180204 (Cat. No. 328007) in DMSO.
Molar mass  327.3
Protect from Light  是的
Hygrocopic  是的
Packaged under inert gas  是的
Purity  ≥97% by HPLC
Chemical formula  C18H13N7
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Irritant
安全标示
R Phrase  R: 36/38
Irritating to eyes and skin.

-20°C

BML-SA278-0100   ERK1/2, pAb   品牌 Biomol

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ERK1/2, pAb

货号： BML-SA278-0100      产品名称： ERK1/2, pAb   品牌： Biomol 规格： 100 ul 3周到货 生化实验

ERK1/2, pAb
Product Specification
Alternative Name:MAPK2/3, MAP kinase 2/3
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 317-339 of human ERK1 (MAPK). This sequence is conserved in human and rat.
UniProt ID:P27361 (Erk1), P28482 (Erk2)
Application:Western Blot
Specificity:Recognizes human, mouse, rat and bovine ERK1/2.
Formulation:Liquid. In phosphate buffer containing BSA and sodium azide.
Long Term Storage:-20°C

-20°C

BML-SE343-0005   Erk1 (human) (recombinant)   品牌 Biomol

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Erk1 (human) (recombinant)

货号： BML-SE343-0005      产品名称： Erk1 (human) (recombinant)   品牌： Biomol 规格： 5 ug 3周到货 生化实验

Erk1 (human), (recombinant)
Product Specification
Alternative Name:MAPK3, MAP kinase 3
MW:45 kDa
Source:Produced in insect cells. Active, full length Erk1 without a tag.
UniProt ID:P27361
Application:Kinetic and functional studies, phosphorylation of target substrates, drug screening.
Long Term Storage:-80°C
Miscellaneous/General:Erk1 is a serine/ threonine kinase that is a member of the extracellular signal-regulated kinase family, which are activated in response to many growth factors and cytokines.

-80°C

BML-SE343-0020   Erk1 (human) (recombinant)   品牌 Biomol

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Erk1 (human) (recombinant)

货号： BML-SE343-0020      产品名称： Erk1 (human) (recombinant)   品牌： Biomol 规格： 20 ug 3周到货 生化实验

Erk1 (human), (recombinant)
Product Specification
Alternative Name:MAPK3, MAP kinase 3
MW:45 kDa
Source:Produced in insect cells. Active, full length Erk1 without a tag.
UniProt ID:P27361
Application:Kinetic and functional studies, phosphorylation of target substrates, drug screening.
Long Term Storage:-80°C
Miscellaneous/General:Erk1 is a serine/ threonine kinase that is a member of the extracellular signal-regulated kinase family, which are activated in response to many growth factors and cytokines.

-80°C

04-349   anti-Erk2 p42 MAPK (C-term)   品牌 Millipore

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anti-Erk2 p42 MAPK (C-term)

货号： 04-349      产品名称： anti-Erk2 p42 MAPK (C-term)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MAP Kinase 2/Erk2, clone E460, Rabbit Monoclonal
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, ICC, IP, FC, IH(P) Rabbit Unpurified Monoclonal Antibody
Description:
Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal
Promotional Text:
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100% Performance Guaranteed
Molecular Weight:
~42 kDa observed
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to the C-terminus of human MAP Kinase 2/Erk2.
Clone:
E460
Isotype:
IgG
Background Information:
Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen activated protein kinase (MAPK) is involved in eukaryotic signal transduction (1). Activated by a dual phosphorylation on threonine and tyrosine residue by the dual-specificity kinase MKK1/2, ERK2 p42 translocate to the nucleus after stimulation (2). Once phosphorylated, ERK2 can form dimers with phosphorylated and unphosphorylated ERK2 partners. It is also able to phosphorylate multiple substrates such as EGFR, c-PLA2, Elk1/TCF and c-Myc (3-5).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat.
Application Notes:
Immunohistochemistry Analysis: A 1:250-500 dilution from a representative lot was used in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC.
Immunoprecipitation Analysis: A 1:80 dilution from a representative lot was used in IP.
Flow Cytometry Analysis: A 1:50 dilution from a representative lot was used in FC.
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Control:
A431 cell lysate
Quality Assurance:
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected MAP Kinase 2/Erk2 in A431 cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P28482
Entrez Gene Number:
P28482
Gene Symbol:
ERK2
PRKM1
ERK
MAPK2
p41mapk
ERK-2
ERT1
PRKM2
MAPK 2
p42-MAPK
EC 2.7.11.24
OTTHUMP00000028754
OTTHUMP00000174492
P42MAPK
p38
p40
p41
MAPK 1
EC 2.7.11
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Alternate Names:
mitogen-activated protein kinase 1
Extracellular signal-regulated kinase 2
Mitogen-activated protein kinase 2
MAP kinase 1
MAP kinase 2
MAP kinase isoform p42
protein tyrosine kinase ERK2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro dia -20°C

BML-SE463-0100   MKP-2/Erk1 (human) (recombinant)   品牌 Biomol

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MKP-2/Erk1 (human) (recombinant)

货号： BML-SE463-0100      产品名称： MKP-2/Erk1 (human) (recombinant)   品牌： Biomol 规格： 100 ug 3周到货 生化实验

MKP-2/Erk1 (human), (recombinant)
Approximately 1:1 stoichiometric complex of MKP-2 with Erk1.
Product Specification
Alternative Name:MAP kinase phosphatase 2/Erk1, DUSP4/Erk1, Dual specificity protein phosphatase 4/Erk1, HVH2/Erk1
MW:42.3 and 43.1 kDa
Source:Produced in Sf9 cells.
UniProt ID:Q13115 (MKP2), P27361 (Erk1)
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, 200mM NaCl, 5mM DTT, 0.1 % Tween-20, 10% glycerol.
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
Specific Activity:≥40 U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute.
Long Term Storage:-80°C
Miscellaneous/General:MKP-2 (DUSP4) has an N-terminal MAP kinase binding domain. Co-expressing MKP-2 with Erk1 MAP kinase results in increased phosphatase activity compared to MKP-2 expressed alone.

-80°C