Anti-Notch 2, cleaved N-terminus (rabbit polyclonal)

货号： 07-1234      产品名称： Anti-Notch 2, cleaved N-terminus (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Notch 2 Antibody, NT
Recommended Replacement for: 07-1234 is a recommended replacement for part AB5713
Species Reactivity Key Applications Host Format Antibody Type
H, M  ELISA, WB, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Notch 2 Antibody, NT
Replaces:
07-1234 is a recommended replacement for part AB5713
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Specificity:
Notch 2, cleaved N terminal. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.
Molecular Weight:
~72 kDa
Epitope:
Cleaved N-terminus
Immunogen:
Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 2.
Isotype:
IgG
Background Information:
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse. The antibody is also expected to react with rat due to immunogen sequence homology (14/15). Other species have not been tested.
Application Notes:
ELISA:
1:30,000-1:90,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of NOTCH-2 Polyclonal Antibody: Squamous Cell Carcinoma
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Control:
NIH/3T3 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH2 on 10 μg of NIH/3T3 lysates.
Presentation:
Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium phosphate buffer with 0.15 M NaCl and 0.1% sodium azide
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
Q04721
Entrez Gene Number:
NM_024408.2
Gene Symbol:
NOTCH2
hN2
OTTHUMP00000059536
AGS2
Alternate Names:
Neurogenic locus notch homolog protein 2 precursor
Notch (Drosophila -20°C