标签归档：gdi

4814-5   D4-GDI, human recombinant   品牌 Biovision

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D4-GDI, human recombinant

货号： 4814-5      产品名称： D4-GDI, human recombinant   品牌： Biovision 规格： 5 μg 3周到货 生化实验

D4-GDI, human recombinant
Inhibits the release of GDP from the Rho proteins
Product Overview
Alternate Names / Synonyms: D4-GDI, D4GDI, Rho GDP dissociation inhibitor (GDI) alpha
Gene Symbol: ARHGDIA
Accession #: NM_004309
Gene ID: 396
Source: E. coli
Appearance: Liquid
Physical Form Description: Sterile filtered liquid (100 µg/ml) in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% Glycerol.
Molecular Weight: 54 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: ≥90%
Endotoxin Level: <0.1 ng/μg
Biological Activity: Not available at this time
Reconstitution Instructions: N/A
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: D4-GDI belongs to the family of rho-GDI or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of rhoGDI-1, D4-GDI/rhoGDI-2 and rhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and is involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas-mediated apoptosis.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

06-730   A HU RHO-GDI PC 12-305   品牌 Millipore

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A HU RHO-GDI PC 12-305

货号： 06-730      产品名称： A HU RHO-GDI PC 12-305   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-RhoGDI Antibody
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REFERENCES
RhoGDI-3 is a new GDP dissociation inhibitor (GDI). Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG.
Zalcman, G, et al. (1996) J. Biol. Chem., 271: 30366-74 (1996)
Defective Rho GTPase regulation by IL-1 beta-converting enzyme-mediated cleavage of D4 GDP dissociation inhibitor.
Danley, D E, et al. (1996) J. Immunol., 157: 500-3 (1996)
D4-GDI, a substrate of CPP32, is proteolyzed during Fas-induced apoptosis.
Na, S, et al. (1996) J. Biol. Chem., 271: 11209-13 (1996)
RhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas.
Adra, C N, et al. (1997) Proc. Natl. Acad. Sci. U.S.A., 94: 4279-84 (1997)
Species Reactivity Key Applications Host Format Antibody Type
B, H, M  IP, WB Rabbit Purified Polyclonal Antibody
Description:
Anti-RhoGDI Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Rho-GDI; cross-reacts with D4-GDI.
Molecular Weight:
28 kDa
Immunogen:
GST fusion protein corresponding to full length Human Rho-GDI
Isotype:
IgG
Species Reactivity:
Bovine
Human
Mouse
Control:
Positive Control Included: 3T3 Cell Lysate (12-305)
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from human A431, HL-60, HeLa, Hs294T, Jurkat or murine 3T3/NIH cells
Purification Method:
Protein A purfied
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P52565
Entrez Gene Number:
NM_004309
Gene Symbol:
ARHGDIA
MGC117248
RHOGDI-1
GDIA1
RHOGDI
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P52565 # Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (By similarity).
SIZE: 204 amino acids; 23207 Da
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: SwissProt: P52565 ## Belongs to the Rho GDI family.
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Brand Family:
Upstate
Product Name:
Anti-RhoGDI Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Rabbit
Applications:
Detect RhoGDI using this Anti-RhoGDI Antibody validated for use in IP & WB.

-20°C