ANTI-CLEAVED BID (AB-1) RABBIT PAB
货号： PC645-10T 产品名称： ANTI-CLEAVED BID (AB-1) RABBIT PAB 品牌： Merck 规格： *** 3周到货 生化实验
Anti-Cleaved Bid (Ab-1) Rabbit pAb
Recognizes the ~15 kDa cleaved Bid protein. Does not recognize uncleaved, full-length Bid.
产品信息
Host Rabbit
Isotype IgG
Immunogen a synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
Form Liquid
Formulation In Dulbecco’s PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Species reactivity not human, mouse
Preservative ≤0.1% sodium azide
Applications
Immunoblotting (Western Blotting)
Avoid freeze/thaw 是的
Positive control TNF-α-treated L929 cells
商店和运输信息
Storage -20°C
Ship Blue Ice Only
Standard Handling
-20°C
B12020 Anti-BID, mouse pAb 品牌 Agilent
Anti-BID, mouse pAb
货号： B12020 产品名称： Anti-BID, mouse pAb 品牌： Agilent 规格：
ADI-AAM-141-E BID, mAb (5C9) 品牌 Enzo
BID, mAb (5C9)
货号： ADI-AAM-141-E 产品名称： BID, mAb (5C9) 品牌： Enzo 规格： 100 ug
632419 pDsRed2-Bid Vector 品牌 clontech
pDsRed2-Bid Vector
货号： 632419 产品名称： pDsRed2-Bid Vector 品牌： clontech 规格： 20 μg
MA515216 Anti-BID 品牌 Pierce
Anti-BID
货号： MA515216 产品名称： Anti-BID 品牌： Pierce 规格： 100 μl
04-438 anti-Bid rabbit monoclonal 品牌 Millipore
anti-Bid rabbit monoclonal
货号： 04-438 产品名称： anti-Bid rabbit monoclonal 品牌： Millipore 规格： EA 3周到货 生化实验
Anti-Bid Antibody, rabbit monoclonal
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REFERENCES
Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death
Gross, A, et al (1999) J Biol Chem, 274:1156-63 (1999)
Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors
Luo, X, et al (1998) Cell, 94:481-90 (1998)
Species Reactivity Key Applications Host Format Antibody Type
H FC, IF, IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Bid Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region before the BH3 domain of human Bid
Molecular Weight:
22 kDa
Immunogen:
KLH-conjugated synthetic peptide before the BH3 domain of human Bid.
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P55957
Entrez Gene Number:
NM_001196.2
NM_197966.1
NM_197967.1
Gene Symbol:
BID
FP497
MGC42355
MGC15319
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
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UniProt Summary:
FUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SIZE: 195 amino acids; 21995 Da
SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic. & BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitocho -20°C
ALX-210-006-R050 Bid (human), pAb (AR-54) 品牌 Alexis
Bid (human), pAb (AR-54)
货号： ALX-210-006-R050 产品名称： Bid (human), pAb (AR-54) 品牌： Alexis 规格： 50.micro.l 三周 生化实验
Bid (human), pAb (AR-54)
Product Specification
Alternative Name: BH3 interacting domain death agonist
Host: Rabbit
Immunogen: Synthetic peptide corresponding to aa 28-50 (C28SDNSFRRELDALGHELPVLAPQ50) of human Bid.
UniProt ID: P55957
Application: Immunohistochemistry (paraffin sections (1:3’000))
Western Blot (1:1’000-1:5’000)
Specificity: Recognizes human, mouse and rabbit Bid. Detects a band of ~22kDa by Western blot.
Formulation: Liquid. In PBS containing 0.05% sodium azide.
Use/Stability: Stable for at least 1 year after receipt when stored at -80°C.
Handling: Avoid freeze/thaw cycles.
Long Term Storage: -80°C
-80°C
ALX-210-007-R050 Bid (mouse), pAb (AR-52) 品牌 Alexis
Bid (mouse), pAb (AR-52)
货号： ALX-210-007-R050 产品名称： Bid (mouse), pAb (AR-52) 品牌： Alexis 规格： 50.micro.l 三周 生化实验
Bid (human), pAb (AR-54)
Product Specification
Alternative Name: BH3 interacting domain death agonist
Host: Rabbit
Immunogen: Synthetic peptide corresponding to aa 28-50 (C28SDNSFRRELDALGHELPVLAPQ50) of human Bid.
UniProt ID: P55957
Application: Immunohistochemistry (paraffin sections (1:3’000))
Western Blot (1:1’000-1:5’000)
Specificity: Recognizes human, mouse and rabbit Bid. Detects a band of ~22kDa by Western blot.
Formulation: Liquid. In PBS containing 0.05% sodium azide.
Use/Stability: Stable for at least 1 year after receipt when stored at -80°C.
Handling: Avoid freeze/thaw cycles.
Long Term Storage: -80°C
-80°C
PA122010 Pierce(R) Anti-BID Rabbit Polyclonal Antibody, 50 uL. 品牌 Pierce
Pierce(R) Anti-BID Rabbit Polyclonal Antibody, 50 uL.
货号： PA122010 产品名称： Pierce(R) Anti-BID Rabbit Polyclonal Antibody, 50 uL. 品牌： Pierce 规格： 50 μl
AB10002 Rabbit anti-Mouse BID Cleavage site spec 品牌 Millipore
Rabbit anti-Mouse BID Cleavage site spec
货号： AB10002 产品名称： Rabbit anti-Mouse BID Cleavage site spec 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-mouse BID cleavage (59/60) site specific Antibody
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REFERENCES
Three novel Bid proteins generated by alternative splicing of the human Bid gene.
Renshaw, Stephen A, et al. (2004) J. Biol. Chem., 279: 2846-55 (2004)
Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
Degli Esposti, Mauro, et al. (2003) J. Biol. Chem., 278: 15749-57 (2003)
Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death
Gross, A, et al (1999) J Biol Chem, 274:1156-63 (1999)
Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis.
Li, H., et al. (1998) Cell , 94:491-501 (1998)
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Species Reactivity Key Applications Host Format Antibody Type
M WB Rabbit Purified Polyclonal Antibody
Description:
Anti-mouse BID cleavage (59/60) site specific Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Mouse 15 kDa caspase-8 cleavage product of BID. Human (75% conserved) BID has been tested and shown not to cross-react. Rat (100% conserved) and chicken (62% conserved) BID have not been tested.
Immunogen:
The antiserum was produced against a chemically synthesized peptide derived from the 15 kDa caspase-8 cleavage product of mouse BID.
Background Information:
BH3 interacting domain death agonist (BID, 22 kDa) is a pro-apoptotic member of the Bcl-2 family. BID interacts with both Bcl-2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase-8 in response to TNF-α or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.
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Species Reactivity:
Mouse
Control:
Mouse L929 (3T3-L1) cells treated with TNF-α to induce cleavage; caspase-8 cleaved recombinant mouse BID.
Presentation:
Dulbecco’s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier, 0.05% sodium azide.
Storage Conditions:
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
UniProt Number:
P70444
Gene Symbol:
BID
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Brand Family:
Chemicon
Product Name:
Anti-mouse BID cleavage (59/60) site specific Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Rabbit
Applications:
This Anti-mouse BID cleavage (59/60) site specific Antibody is validated for use in WB for the detection of mouse BID cleavage (59/60) site specific.
-20℃
3272BP-50 Bid Blocking Peptide 品牌 Biovision
Bid Blocking Peptide
货号： 3272BP-50 产品名称： Bid Blocking Peptide 品牌： Biovision 规格： 50 μg 3周到货 生化实验
Bid Blocking Peptide
Product Overview
Product Name: Bid Blocking Peptide
Antibody Target: Synaptotagmin
Accession #: P21707
Gene ID: 25716
Appearance: Colorless liquid
Concentration: 0.5mg/ml
Formulation: 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Temp.: -20°C
Shipping: Gel Pack
Application & Usage: The peptide is used for blocking the antibody activity of synaptotagmin (Cat. # 3177-100). It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Description: 50 ug blocking peptide for synaptotagmin polyclonal Antibody (Cat. # 3177-100).
Handling: The peptide solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.
-20°C
AB1730 BID, RB X-100UG 品牌 Millipore
BID, RB X-100UG
货号： AB1730 产品名称： BID, RB X-100UG 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Bid (full length) Antibody
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REFERENCES
Survivin expression in glioblastomas correlates with proliferation, but not with apoptosis.
Marta Mellai,Valentina Caldera,Alessia Patrucco,Laura Annovazzi,Davide Schiffer (0001) Anticancer research.28
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Bid (full length) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
AB1730 detects the full length (22 kDa), detection of the 15kDa truncated form is very difficult with AB1730.
Immunogen:
An 18 amino acid synthetic peptide peptide surrounding amino acid 37 of human Bid (ID# BV63-64), corresponding to a region near the N-terminus of human Bid.
Background Information:
Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
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Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western blot: 1 μg/mL
Immunoprecipitation: 5-10 μg/mL
Immunohistochemistry: 20-40 μg/mL
Optimal working dilutions must be determined by end user.
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Presentation:
Affinity purified immunoglobulin in liquid in PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Storage Conditions:
Maintain frozen at -20°C for up to 12 momnths from date of receipt. For optimal storage, aliquot and freeze at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
UniProt Number:
P55957
Entrez Gene Number:
NM_001196.2
NM_197966.1
NM_197967.1
Gene Symbol:
BID
FP497
MGC42355
MGC15319
Alternate Names:
BH3 Interacting Domain Death Agonist
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Immunohistochemistry
Entrez Gene Summary:
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
View All »
UniProt Summary:
FUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SIZE: 195 amino acids; 21 -20℃
AB1735 BID, RBX -100UL 品牌 Millipore
BID, RBX -100UL
货号： AB1735 产品名称： BID, RBX -100UL 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Bid Antibody
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Rb WB, IP, IHC Rabbit Serum Polyclonal Antibody
Description:
Anti-Bid Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
AB1735 recognizes Bid from human, mouse, rat and rabbit.
Immunogen:
Recombinant full length GST-tagged mouse Bid protein
Background Information:
Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
View All »
Species Reactivity:
Human
Mouse
Rat
Rabbit
Application Notes:
Western blot: Recommended antibody dilution 1:3,000 to 1:6,000. Recognizes a band migrating at approximately 22 kDa corresponding to Bid.
Immunoprecipitation: Recommended antibody dilution 1:200
Immunohistochemistry: Recommended antibody dilution 1:1500
Optimal working dilutions must be determined by end user.
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Presentation:
Whole serum without preservatives
Storage Conditions:
Store at -20°C for up to 12 months.
UniProt Number:
P55957
Entrez Gene Number:
NM_001196.2
NM_197966.1
NM_197967.1
Gene Symbol:
BID
FP497
MGC42355
MGC15319
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunoprecipitation
Immunohistochemistry
Entrez Gene Summary:
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
View All »
UniProt Summary:
FUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SIZE: 195 amino acids; 21995 Da
SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic. & BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitochondria as an integral membrane protein (By similarity). & BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note= -20℃
MA113043 Pierce BID Mouse (23F7) Monoclonal, 0.1 mg (100 uL) 品牌 Pierce
Pierce BID Mouse (23F7) Monoclonal, 0.1 mg (100 uL)
货号： MA113043 产品名称： Pierce BID Mouse (23F7) Monoclonal, 0.1 mg (100 uL) 品牌： Pierce 规格： 100 UL
AB2994 Anti-Bid (pS65) Polyclonal Ab 品牌 Millipore
Anti-Bid (pS65) Polyclonal Ab
货号： AB2994 产品名称： Anti-Bid (pS65) Polyclonal Ab 品牌： Millipore 规格： EA 三周到货 生化实验