标签： rock

V3411   ROCK1 Kinase Enzyme System   品牌 Promega

ROCK1 Kinase Enzyme System

货号： V3411      产品名称： ROCK1 Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 GST 标签的重组人源 ROCK1 （第 17 位 – 第 535 位氨基酸）。 ROCK1 是一个广泛表达的丝氨酸和苏氨酸激酶，是小 GTP 酶 RhoA 的下游靶标。 ROCK1 参与了不同的细胞功能，包括平滑肌收缩、激动蛋白细胞骨架组装、细胞黏附和运动、基因表达。在心肌细胞应对病理刺激时， ROCK1 可引起心肌纤维化的发展及对纤维化细胞因子的诱导。 ROCK1 基因敲除小鼠表现出血管周围及细胞间隙纤维化的降低，这与多种胞外基质（ ECM ）蛋白及纤维化细胞因子的表达量降低有关。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： ROCK1 , 10μg ( 人源，重组， 第 17 位 – 第 535 位氨基酸 ) ； MW : ~85kDa 。
•  底物： S6K 底物（ KRRRLASLR ）；从人 40S 核糖体蛋白 S6 （第 230 – 第 238 位氨基酸）衍生而来 。
其它组分： 反应缓冲液， DTT 。
ROCK1 NCBI 数据库入口。
人类激酶酶系统及其应用。 
特点  •  在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2 。
•  可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 

04-1121   Anti-ROCK1, clone EPR638Y   品牌 Millipore

Anti-ROCK1, clone EPR638Y

货号： 04-1121      产品名称： Anti-ROCK1, clone EPR638Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IH(P), WB, ICC, IP Rabbit Unpurified Monoclonal Antibody
Description:
Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal
Promotional Text:
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Specificity:
This antibody recognizes ROCK-1.
Molecular Weight:
~ 160 kDa
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to residues near the C-terminus of human ROCK-1.
Clone:
EPR638Y
Isotype:
IgG
Background Information:
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, and rat.
Application Notes:
Immunohistochemistry Analysis: 1:50-100 dilution from a previous lot detected ROCK-1 in human lymphoma tissue.
Immunocytochemistry: 1:50-100 dilution from a previous lot was used in IC.
Immunoprecipitation: 1:60 dilution from a previous lot was used in IP.
Control:
HeLa cell lysate.
Quality Assurance:
Evaluated by Western Blot on HeLa cell lysates.
Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect ROCK-1 in HeLa cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-glycine (pH 7.4), 0.15 M NaCl containing 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q13464
Entrez Gene Number:
Q13464
Gene Symbol:
EC 2.7.11.1
MGC131603
MGC43611
OTTHUMP00000162514
P160ROCK
PRO0435
p160-ROCK
p160ROCK
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Alternate Names:
Renal carcinoma antigen NY-REN-35
Rho-associated, coiled-coil containing protein kinase 1
Rho-associated, coiled-coil-containing protein kinase 1
p160 ROCK-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Immunoprecipitation
UniProt Summary:
FUNCTION: Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction a -20°C

04-841   A-ROKA/ROCK-II, CLONE A9W4 100ul   品牌 Millipore

A-ROKA/ROCK-II, CLONE A9W4 100ul

货号： 04-841      产品名称： A-ROKA/ROCK-II, CLONE A9W4 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-ROKα/ROCK-II, clone A9W4
Recommended Replacement for: 05-841
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REFERENCES
The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.
Vincent, S and Settleman, J (1997) Mol. Cell. Biol., 17: 2247-56 (1997)
The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton.
Leung, T, et al. (1996) Mol. Cell. Biol., 16: 5313-27 (1996)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, B  IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
Replaces:
05-841
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
Molecular Weight:
150 kDa
Immunogen:
6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.
Clone:
A9W4
Background Information:
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
View All »
Species Reactivity:
Human
Mouse
Rat
Bovine
Species Reactivity Note:
Human and rat. Predicted to cross-react with mouse and bovine based on sequence homology.
Application Notes:
Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
Control:
RIPA lysates from PC-12 cells
Quality Assurance:
Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Presentation:
Cultured supernatant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q62868
Entrez Gene Number:
NM_013022
Gene Symbol:
EC 2.7.11.1
MGC131603
MGC43611
OTTHUMP00000162514
P160ROCK
PRO0435
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Alternate Names:
RhoA-binding kinase 2
Rho-associated, coiled-coil containing protein kinase 2
Rho-associated, coiled-coil-containing protein kinase II
p150 ROK-alpha
p164 ROCK-2
ROCK-II
ROKalpha
Rock2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
A Rho kinase that is involved in inducing membrane blebbing and chromatin -20°C

ABS436   Anti-ROCK2   品牌 Millipore

Anti-ROCK2

货号： ABS436      产品名称： Anti-ROCK2   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-ROCK2
Species Reactivity Key Applications Host Format Antibody Type
M  WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-ROCK2 Antibody
Molecular Weight:
~160 kDa observed. Uncharacterized bands may appear in some lysate(s).
Immunogen:
KLH-conjugated linear peptide corresponding to human ROCK2.
Background Information:
Rho-associated protein kinase 2 (ROCK2), also known as Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase II, and p164 ROCK-2, is a member of the AGC serine/threonine protein kinase family. ROCK2 is a key regulator of actin cytoskeleton and cell polarity. It is involved in regulation of actin cytoskeleton organization, smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, stress fiber and focal adhesion formation, neurite retraction, motility and cell adhesion. Many of the key roles of ROCK2 are attributed to its ability to phosphorylate ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A, VIM, SORL1 and IRF4. ROCK2 regulates positively the activation of p42/MAPK1-p44/MAPK3 and the regulation of p90RSK/RPS6KA1 during myogenic differentiation. Alternatively, ROCK2 is a negative regulator of VEGF-induced angiogenic endothelial cell activation. Recent studies have shown that ROCK2 inhibits keratinocyte terminal differentiation and may play an important role in the timely initiation of centrosome duplication. It also may regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Additional evidence suggests that ROCK2 plays a critical role in the regulation of spine and synaptic properties in the hippocampus.
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Species Reactivity:
Mouse
Application Notes:
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ROCK2 in mouse brain tissue.
Control:
Mouse brain tissue lysate
Quality Assurance:
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected ROCK2 in 15 µg of mouse brain tissue lysate.
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O75116
Gene Symbol:
ROCK2
Alternate Names:
Rho-associated coiled-coil containing protein kinase 2
Rho-associated protein kinase 2
Rho-associated, coiled-coil-containing protein kinase II
p164 ROCK-2
ROCK2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-ROCK2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-ROCK2 Antibody is a highly specific rabbit polyclonal antibody, that targets Rho-associated protein kinase 2 & has been tested in western blotting & IHC.

2-8℃