标签归档：brdu

QIA58-1000TEST   BRDU CELL PROLIFERATION ASSAY   品牌 Merck

发表于由

BRDU CELL PROLIFERATION ASSAY

货号： QIA58-1000TEST      产品名称： BRDU CELL PROLIFERATION ASSAY   品牌： Merck 规格： *** 3周到货 生化实验

BrdU Cell Proliferation Assay
Bromodeoxyuridine Assay
BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform. Note: 1 T = 1 test.
产品信息
Format  96-well plate
Form  200 or 1000 Tests
Detection method  Colorimetric
Avoid freeze/thaw  是的
Assay time  3 h
Sample type  Intact Cells
Kit contains  BrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA58-200TEST   BRDU CELL PROLIFERATION ASSAY   品牌 Merck

发表于由

BRDU CELL PROLIFERATION ASSAY

货号： QIA58-200TEST      产品名称： BRDU CELL PROLIFERATION ASSAY   品牌： Merck 规格： *** 3周到货 生化实验

BrdU Cell Proliferation Assay
Bromodeoxyuridine Assay
BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform. Note: 1 T = 1 test.
产品信息
Format  96-well plate
Form  200 or 1000 Tests
Detection method  Colorimetric
Avoid freeze/thaw  是的
Assay time  3 h
Sample type  Intact Cells
Kit contains  BrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

B5002   热销|5-BrdU|Brdu|5-bromo-2—deoxyuridine|5-溴-2-脱氧脲苷   品牌 Sigma

发表于由

热销|5-BrdU|Brdu|5-bromo-2—deoxyuridine|5-溴-2-脱氧脲苷

货号： B5002      产品名称： 热销|5-BrdU|Brdu|5-bromo-2—deoxyuridine|5-溴-2-脱氧脲苷   品牌： Sigma 规格： 100MG 大量现货

英文名: Brdu,5-bromo-2—deoxyuridine
中文名：5-溴脱氧尿嘧啶核苷
分子式：C9H11BrN2O5
分子量：307.10
外观：白色结晶粉末
Brdu，中文全名5-溴脱氧尿嘧啶核苷，为胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活体注射或细胞培养加入，而后利用抗Brdu单克隆抗体，ICC染色，显示增殖细胞。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。
EdU是一种胸腺嘧啶核苷类似物，能够在细胞增殖时期代替胸腺嘧啶（T）渗入正在复制的DNA分子，通过基于EdU与Apollo荧光染料的特异性反应检测DNA复制活性，通过检测EdU标记便能准确地反映细胞的增殖情况。与BrdU检测方法相比，EdU检测方法更快速、更灵敏、更准确。EdU检测染料只有BrdU抗体大小的1/500
，在细胞内很容易扩散，无需DNA变性（酸解、热解、酶解等）即可有效检测，可有效避免样品损伤，在细胞和组织水平能更准确地反映细胞增殖等现象。而BrdU需要变性DNA后才能与抗体结合，破坏了DNA双链结构，影响了Hochest等染色，导致染色弥散，准确性降低等问题，逐渐被EdU取代.
Sigma相关产品资料：
Sigma  B5002 5-Bromo-2′-deoxyuridine ≥99% (HPLC)
Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
CAS Number: 59-14-3
Linear Formula: C9H11BrN2O5
Molecular Weight: 307.10
Beilstein Registry Number: 30395
EC Number: 200-415-9
MDL number: MFCD00006529
PubChem Substance ID: 24278270
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions  are available for this Product.
Biochem/physiol Actions Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
性质
assay ≥99% (HPLC)
mp 191-194 °C (dec.)(lit.)
storage temp. −20°C

-20°C

B5002   现货|5-BrdU|Brdu|5-Bromo-2′-deoxyuridine|5-溴-2-脱氧脲苷   品牌 Sigma

发表于由

现货|5-BrdU|Brdu|5-Bromo-2′-deoxyuridine|5-溴-2-脱氧脲苷

货号： B5002      产品名称： 现货|5-BrdU|Brdu|5-Bromo-2′-deoxyuridine|5-溴-2-脱氧脲苷   品牌： Sigma 规格： 500MG 大量现货

英文名: Brdu,5-bromo-2—deoxyuridine
中文名：5-溴脱氧尿嘧啶核苷
分子式：C9H11BrN2O5
分子量：307.10
外观：白色结晶粉末
Brdu，中文全名5-溴脱氧尿嘧啶核苷，为胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活体注射或细胞培养加入，而后利用抗Brdu单克隆抗体，ICC染色，显示增殖细胞。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。
EdU是一种胸腺嘧啶核苷类似物，能够在细胞增殖时期代替胸腺嘧啶（T）渗入正在复制的DNA分子，通过基于EdU与Apollo荧光染料的特异性反应检测DNA复制活性，通过检测EdU标记便能准确地反映细胞的增殖情况。与BrdU检测方法相比，EdU检测方法更快速、更灵敏、更准确。EdU检测染料只有BrdU抗体大小的1/500
，在细胞内很容易扩散，无需DNA变性（酸解、热解、酶解等）即可有效检测，可有效避免样品损伤，在细胞和组织水平能更准确地反映细胞增殖等现象。而BrdU需要变性DNA后才能与抗体结合，破坏了DNA双链结构，影响了Hochest等染色，导致染色弥散，准确性降低等问题，逐渐被EdU取代.
Sigma相关产品资料：
Sigma  B5002 5-Bromo-2′-deoxyuridine ≥99% (HPLC)
Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
CAS Number: 59-14-3
Linear Formula: C9H11BrN2O5
Molecular Weight: 307.10
Beilstein Registry Number: 30395
EC Number: 200-415-9
MDL number: MFCD00006529
PubChem Substance ID: 24278270
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions  are available for this Product.
Biochem/physiol Actions Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
性质
assay ≥99% (HPLC)
mp 191-194 °C (dec.)(lit.)
storage temp. −20°C

−20°C

B5002   批发|5-BrdU|Brdu|5-bromo-2—deoxyuridine|5-溴-2-脱氧脲苷|   品牌 Sigma

发表于由

批发|5-BrdU|Brdu|5-bromo-2—deoxyuridine|5-溴-2-脱氧脲苷|

货号： B5002      产品名称： 批发|5-BrdU|Brdu|5-bromo-2—deoxyuridine|5-溴-2-脱氧脲苷|   品牌： Sigma 规格： 1g 大量现货

英文名: Brdu,5-bromo-2—deoxyuridine
中文名：5-溴脱氧尿嘧啶核苷
分子式：C9H11BrN2O5
分子量：307.10
外观：白色结晶粉末
Brdu，中文全名5-溴脱氧尿嘧啶核苷，为胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活体注射或细胞培养加入，而后利用抗Brdu单克隆抗体，ICC染色，显示增殖细胞。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。
EdU是一种胸腺嘧啶核苷类似物，能够在细胞增殖时期代替胸腺嘧啶（T）渗入正在复制的DNA分子，通过基于EdU与Apollo荧光染料的特异性反应检测DNA复制活性，通过检测EdU标记便能准确地反映细胞的增殖情况。与BrdU检测方法相比，EdU检测方法更快速、更灵敏、更准确。EdU检测染料只有BrdU抗体大小的1/500
，在细胞内很容易扩散，无需DNA变性（酸解、热解、酶解等）即可有效检测，可有效避免样品损伤，在细胞和组织水平能更准确地反映细胞增殖等现象。而BrdU需要变性DNA后才能与抗体结合，破坏了DNA双链结构，影响了Hochest等染色，导致染色弥散，准确性降低等问题，逐渐被EdU取代.
Sigma相关产品资料：
Sigma  B5002 5-Bromo-2′-deoxyuridine ≥99% (HPLC)
Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
CAS Number: 59-14-3
Linear Formula: C9H11BrN2O5
Molecular Weight: 307.10
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions  are available for this Product.
Biochem/physiol Actions Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
性质
assay ≥99% (HPLC)
mp 191-194 °C (dec.)(lit.)
storage temp. −20°C

-20度

05-633   ANTI-BRDU, CL BU-1 4 VLS   品牌 Millipore

发表于由

ANTI-BRDU, CL BU-1 4 VLS

货号： 05-633      产品名称： ANTI-BRDU, CL BU-1 4 VLS   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-BrdU Antibody, clone BU-1
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REFERENCES
Difference between random and imprinted X inactivation in common voles.
Elena V Dementyeva,Alexander I Shevchenko,Olga V Anopriyenko,Nina A Mazurok,Eugeny A Elisaphenko,Tatyana B Nesterova,Neil Brockdorff,Suren M Zakian (2010) Chromosoma.119
Immunofluorescence labeling indices in myeloma and related monoclonal gammopathies.
Greipp, P R, et al. (1987) Mayo Clin. Proc., 62: 969-77 (1987)
S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment.
Gonchoroff, N J, et al. (1986) J. Immunol. Methods, 93: 97-101 (1986)
A monoclonal antibody reactive with 5-bromo-2-deoxyuridine that does not require DNA denaturation.
Gonchoroff, N J, et al. (1985) Cytometry, 6: 506-12 (1985)
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Species Reactivity Key Applications Host Format Antibody Type
A  ICC Mouse Culture Supernatant Monoclonal Antibody
Description:
Anti-BrdU Antibody, clone BU-1
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
5-Bromo-2-deoxyuridine
Molecular Weight:
none given
Immunogen:
5-iodouridine:ovalbumin conjugate
Isotype:
IgG2a
Background Information:
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
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Species Reactivity:
All
Quality Assurance:
A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
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Purification Method:
Concentrated culture supernatant
Presentation:
4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.
Storage Conditions:
2 years at -20°C
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Brand Family:
Upstate
Product Name:
Anti-BrdU Antibody, clone BU-1
Antibody Type:
Monoclonal Antibody
Qty/Pk:
4 vials
Format:
Culture Supernatant
Host:
Mouse
Applications:
Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.< -20°C