标签归档：pkd

ST1042-50UG   ANTI-PKD2 RABBIT PAB   品牌 Merck

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ANTI-PKD2 RABBIT PAB

货号： ST1042-50UG      产品名称： ANTI-PKD2 RABBIT PAB   品牌： Merck 规格： *** 3周到货 生化实验

Anti-PKD2 Rabbit pAb
Anti-Protein Kinase D2
Recognizes the ~105 kDa PKD2 protein in HEK293 cells.
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic peptide corresponding to amino acids near the C-terminus of human PKD2
Form  Liquid
Formulation  In PBS.
Species reactivity  human
Preservative  ≤0.1% sodium azide
Applications 
Immunoblotting (Western Blotting)
Immunoprecipitation
Positive control  HEK 293 cells
商店和运输信息
Storage  +2°C to +8°C
Ship  Blue Ice Only
Highly Toxic

2°C – 8°C

7711-5   PKD2, Active   品牌 Biovision

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PKD2, Active

货号： 7711-5      产品名称： PKD2, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active PKD2
Inactivates the enzyme pyruvate dehydrogenase
Product Overview
Alternate Names / Synonyms: pdk-2, pdk 2, Active PKD2, PKD2, human PKD2, active kinase, active kinases, PKD, recombinant PKD2, recombinant PKD, kinase activity assay, protein kinase
Gene Symbol: PKD2
Accession #: Q13563
Gene ID: 5311
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: ~130.0 kDa
Purity by SDS-PAGE: ≥80%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 259 nmol phosphate incorporated into CREBtide substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: gel pack
Background Information: PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain (1). It exhibits the strongest homology to the serine/threonine protein kinases PKD/PKCmu and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation (2).PKD2 can be activated by phorbol esters both in vivo and in vitro but also by gastrin via the cholecystokinin/CCK(B) receptor in human gastric cancer cells stably transfected with the CCK(B)/gastrin receptor (AGS-B cells) (3). Gastrin-stimulated PKD2 activation involves a heterotrimeric G α(q) protein as well as the activation of phospholipase C. Furthermore, PKD2 can be activated by classical and novel members of the protein kinase C (PKC) family such as PKC α, PKC epsilon, and PKC eta implicating PKD2 to be a downstream target of specific PKCs upon the stimulation of AGS-B cells with gastrin . PKD2 is predominantly cytoplasmic and stimulation of cells with the G protein-coupled receptor agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKD2 by a mechanism that requires PKC activity (4). In contrast to the other PKD isoenzymes, PKD2 activation does not induce its redistribution from the cytoplasm to the nucleus.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

07-2177   Anti-phospho-PKD2 (Ser197/Ser200)   品牌 Millipore

发表于由

Anti-phospho-PKD2 (Ser197/Ser200)

货号： 07-2177      产品名称： Anti-phospho-PKD2 (Ser197/Ser200)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-PKD2 Antibody (Ser197/Ser200)
Description:
Anti-phospho-PKD2 Antibody (Ser197/Ser200)
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes PKD2 phosphorylated at Ser197/Ser200.
Molecular Weight:
~110 kDa observed. An uncharacterized band appears at ~150 kDa in some lysates.
Epitope:
Phosphorylated Ser197/Ser200
Immunogen:
KLH-conjugated linear peptide corresponding to human PKD2 phosphorylated at Ser197/Ser200.
Modifications:
Phosphorylation
Phosphorylation
Background Information:
As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.
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Species Reactivity:
Mouse
Human
Rat
Species Reactivity Note:
Demonstrated to react with Mouse.
Predicted to react with Human and Rat based on 100% sequence homology.
Application Notes:
Evaluated by Western Blot in MEF cell lysates untreated and treated with PDBu, Rapamycin, and GO6983.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected PKD2 in 10 µg of MEF cell lysates untreated and treated with PDBu, Rapamycin, and GO6983.
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Control:
MEF cell lysates untreated and PBDu-treated
Quality Assurance:
Evaluated by Western Blot in MEF cell lysates untreated and PBDu-treated.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected PKD2 in 10 µg of MEF cell lysates untreated and PBDu-treated.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13563
Entrez Gene Number:
NP_000288
Gene Symbol:
PC2
PKD4
Pc-2
APKD2
TRPP2
MGC138466
MGC138468
View All »
Alternate Names:
Autosomal dominant polycystic kidney disease type II protein
Polycystic kidney disease 2 protein
Polycystwin
R48321
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the polycystin protein family. The encoded p 2°C – 8°C

04-787   A-PH-PKD (SER916),CL MC29 100UL   品牌 Millipore

发表于由

A-PH-PKD (SER916),CL MC29 100UL

货号： 04-787      产品名称： A-PH-PKD (SER916),CL MC29 100UL   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-PKD (Ser916), clone MC29
Recommended Replacement for: 05-787
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REFERENCES
Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts.
James Sinnett-Smith,Nora Rozengurt,Robert Kui,Carlos Huang,Enrique Rozengurt (2011) The Journal of biological chemistry.286
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Rabbit null Monoclonal Antibody
Description:
Anti-phospho-PKD (Ser916) Antibody, clone MC29 | 04-787
Replaces:
05-787
Promotional Text:
Special Offer on Antibodies! Click Here!
Trade Name:
Upstate (Millipore)
Specificity:
phospho-PKD
Molecular Weight:
~105-110kDa
Immunogen:
peptide containing RVpSIL in which pS corresponds to phospho-serine 916 of mouse PKD
Modifications:
Phosphorylation
Clone:
MC29
Isotype:
IgG
Background Information:
There are two PKD isoforms (PKD1 and PKD2) belonging to the polycystin family. These proteins are involved in protein-protein and protein-carbohydrate adhesion. The two isoforms are known to interact, and also thought to be ion channel regulators. Defects in PKD1 have been identified as the cause of autosomal dominant polycystic kidney disease type I (ADPKD).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human. Predicted to cross-react with mouse based on sequence homology.
Application Notes:
Western Blot Analysis:
1:500-1:2000 of this lot detected PKD in RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate (PDBu) for 10 minutes at 50 ng/mL.
Control:
RIPA lysates from HEK293 cells
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
Q15139
Entrez Gene Number:
NM_002742
Gene Symbol:
PRKD1
PKD
PKCM
nPKC-mu
PRKCM
PKC-mu
nPKC-D1
PKC-MU
PKD1
View All »
Alternate Names:
Protein kinase C mu type
Protein kinase D
polycystic kidney disease 1 (autosomal dominant)
polycystic kidney disease-associated protein
polycystin 1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: Q15139 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
SIZE: 912 amino acids; 101888 Da
SUBUNIT: Interacts via its N-terminal region with CENTA1.
-20°C

07-385   ANTI-PHOS-PKD2 (S876)   品牌 Millipore

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ANTI-PHOS-PKD2 (S876)

货号： 07-385      产品名称： ANTI-PHOS-PKD2 (S876)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PKD2 (Ser876) Antibody
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REFERENCES
Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases.
Sturany, S, et al. (2001) J. Biol. Chem., 276: 3310-8 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-PKD2 (Ser876) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
phospho-PKD2 (Ser876),
Molecular Weight:
105 kDa
Immunogen:
synthetic peptide corresponding to amino acids surrounding phosphorylated Serine 876 of human PKD2
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot in lysates from PDBu (Phorbol 12,13-dibutyrate) stimulated HEK293 cells
Purification Method:
Protein A purfied
Presentation:
0.1Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Q9BZL6
Entrez Gene Number:
NM_016457
Gene Symbol:
PRKD2
DKFZP586E0820
PKD2
nPKC-D2
HSPC187
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms.
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UniProt Summary:
FUNCTION: SwissProt: Q9BZL6 # Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.
SIZE: 878 amino acids; 96722 Da
TISSUE SPECIFICITY: Widely expressed.
PTM: Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.
SIMILARITY: SwissProt: Q9BZL6 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 PH domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
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Brand Family:
Upstate
Product Name:
Anti-phospho-PKD2 (Ser876) Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Rabbit
Applications:
Anti-phospho-PKD2 (Ser876) Antibody detects level of phospho-PKD2 (Ser876) & has been published & validated for use in WB.

-20℃

07-438   ANTI-PHOSPHO-PKD (SER916) 200UG   品牌 Millipore

发表于由

ANTI-PHOSPHO-PKD (SER916) 200UG

货号： 07-438      产品名称： ANTI-PHOSPHO-PKD (SER916) 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PKD (Ser916) Antibody
Close
REFERENCES
Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu.
Matthews, S A, et al. (1999) J. Biol. Chem., 274: 26543-9 (1999)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-PKD (Ser916) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
phosphorylated PKD
Molecular Weight:
105-110kDa
Immunogen:
synthetic peptide corresponding to amino acids surrounding the phosphorylation site Serine 916 of mouse PKD
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from 293 cells stimulated with PDBu for 10 minutes at 50ng/ml
Purification Method:
Protein A purfied
Presentation:
Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Q15139
Entrez Gene Number:
NM_002742
Gene Symbol:
PRKD1
PKD
PKCM
nPKC-mu
PRKCM
PKC-mu
nPKC-D1
PKC-MU
PKD1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
View All »
UniProt Summary:
FUNCTION: SwissProt: Q15139 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
SIZE: 912 amino acids; 101888 Da
SUBUNIT: Interacts via its N-terminal region with CENTA1.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q15139 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 PH domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
View All »
Brand Family:
Upstate
Product Name:
Anti-phospho-PKD (Ser916) Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Rabbit
Applications:
This Anti-phospho-PKD (Ser916) Antibody is validated for use in WB for the detection of phospho-PKD (Ser916).

-20℃