标签归档：pdh

AAMT008-1KIT   PDH Activity Assay Kit   品牌 Merck

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PDH Activity Assay Kit

货号： AAMT008-1KIT      产品名称： PDH Activity Assay Kit   品牌： Merck 规格： *** 3周到货 生化实验

AAMT008  PDH Activity Assay Kit
Product Description
The PDH Activity Assay Kit is used to determine the activity of pyruvate dehydrogenase (PDH) in a sample. The PDH enzyme is immunocaptured within the wells of the microplate and PDH activity is determined by following the reduction of NAD+ to NADH, coupled to the reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (See Figure 1 at the bottom of this page).
PDH is the key regulatory enzyme of cellular metabolism because it links the TCA cycle and subsequent oxidative phosphorylation with glycolysis and gluconeogenesis as well as with both lipid and amino acid metabolism. PDH activity is regulated by PDK-dependent phosphorylation and PDP dependent dephosphorylation of PDH. Phosphorylation inactivates PDH whereas dephosphorylation activates PDH.
The PDH Activity Assay Kit can be used to elucidate various aspects of PDH activity, physiologic regulation and phosphorylation status. Please read TB561 for three possible applications for this kit.
Panel Components
• 15 ml         20X Buffer, (72545-15ML)
• 2 x 1 ml     Detergent, (72546-1ML)
• 2 x 600 µl  20X Reagent Mix, (72547-600UL)
• 1 ea           Microplate, (72548-1EA)
• 13 ml        5X Stabilizer, (72549-1EA)
• 250 µl       100X Coupler, (72550-250UL)
• 250 µl       100X Reagent Dye, (72551-250UL)
*This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store all components at 4°C. Store the 100X Coupler at –80°C. This kit is stable for at least 6 months.
This kit contains the necessary buffers and reagents for the respective reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
商店和运输信息
Storage  Multiple storage requirements
Ship  Multiple Storage Conditions

+4°C

AP1062-50UL   PhosphoDetectAnti-PDH-E1a(pSer293)RabpAb   品牌 Merck

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PhosphoDetectAnti-PDH-E1a(pSer293)RabpAb

货号： AP1062-50UL      产品名称： PhosphoDetectAnti-PDH-E1a(pSer293)RabpAb   品牌： Merck 规格： *** 3周到货 生化实验

PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb
Anti-Pyruvate Dehydogenase pSer293 Rabbit pAb
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
Form  Liquid
Formulation  In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
Species reactivity  human, mouse, primate, rat
Preservative  ≤0.1% sodium azide
Applications 
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Positive control  HEK293 cells
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

-20°C

G6378   现货进口|G-6-PDH|G-6-P-DH|葡萄糖-6-磷酸脱氢酶   品牌 Sigma

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现货进口|G-6-PDH|G-6-P-DH|葡萄糖-6-磷酸脱氢酶

货号： G6378      产品名称： 现货进口|G-6-PDH|G-6-P-DH|葡萄糖-6-磷酸脱氢酶   品牌： Sigma 规格： 500U 现货 测定血液葡萄糖的含量

英文名：G6P-DH，Glucose-6-Phosphate Dehydrogenase，G-6-PDH，G-6-P-DH
中文名：葡萄糖-6-磷酸脱氢酶，6-磷酸葡萄糖脱氢酶
说明：生化研究；测定血液葡萄糖的含量；通过酶的偶联反应测
定血清激酶的含量。
CAS＃：9001-40-5
相对分子质量：约110000
外观：白色结晶
特性：类型：Type V
单位定义： 25 ℃，pH 7.4，在NADP存在条件下每分钟将1.0 μmoleD-葡萄糖-6-磷酸氧化成6-磷酸-D-葡糖酸的酶量为1单位。
Sigma相关产品资料：
Sigma  G6378  Glucose-6-phosphate Dehydrogenase from baker’s yeast (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonym: G-6-P-DH, Zwischenferment
CAS Number: 9001-40-5
Enzyme Commission (EC) Number: 1.1.1.49  ( BRENDA  | IUBMB  )
EC Number: 232-602-6
MDL number: MFCD00081656
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions  are available for this Product.
Preparation Note Prepared from G7877
Unit Definition One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
Physical form Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight
Properties
type Type XV
form lyophilized powder
purified by crystallization
EQP level Premium
storage temp. −20°C

-20°C

G6378   大量现货|G-6-PDH|G-6-P-DH|葡萄糖-6-磷酸脱氢酶   品牌 Sigma

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大量现货|G-6-PDH|G-6-P-DH|葡萄糖-6-磷酸脱氢酶

货号： G6378      产品名称： 大量现货|G-6-PDH|G-6-P-DH|葡萄糖-6-磷酸脱氢酶   品牌： Sigma 规格： 1KU 现货 测定血液葡萄糖的含量

产地：usa
货号：G6378
英文名：G6P-DH，Glucose-6-Phosphate Dehydrogenase，G-6-PDH，G-6-P-DH
中文名：葡萄糖-6-磷酸脱氢酶，6-磷酸葡萄糖脱氢酶
CAS：9001-40-5
相对分子质量：约110000
外观：白色结晶
说明：生化研究；测定血液葡萄糖的含量；通过酶的偶联反应测
定血清激酶的含量。
特性：类型：Type V
单位定义：25 ℃，pH 7.4，在NADP存在条件下每分钟将1.0 μmoleD-葡萄糖-6-磷酸氧化成6-磷酸-D-葡糖酸的酶量为1单位。
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions  are available for this Product.
Preparation Note Prepared from G7877
Unit Definition One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
Physical form Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight
Properties
type Type XV
form lyophilized powder
purified by crystallization
EQP level Premium
storage temp. −20°C

-20°C