Nitrotyrosine ELISA Kit

货号： 17-10006      产品名称： Nitrotyrosine ELISA Kit   品牌： Millipore 规格： EA 三周到货 生化实验

Nitrotyrosine ELISA
Description:
Nitrotyrosine ELISA
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer, and aging. Oxidative stress occurs as a response to increased oxidants, decreased anti-oxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases.
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.
Upstate’s new Nitrotyrosine Assay Kit, Chemiluminescence Detection (Cat. # 17-10006) is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including a pre-coated 96-well plates, Nitrotyrosine peptide standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers.
The assay has a wide dynamic range and high precision, making this assay a valuable new tool for the study of tyrosine nitration.
View All »
Background Information:
Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer and aging. Oxidative stress occurs as a response to increased oxidants, decreased antioxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases.
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.
View All »
RT

Nitrotyrosine ELISA Kit,2-8C

货号： 17-10006-1      产品名称： Nitrotyrosine ELISA Kit,2-8C   品牌： Millipore 规格： EA 三周到货 生化实验

Nitrotyrosine ELISA
Description:
Nitrotyrosine ELISA
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer, and aging. Oxidative stress occurs as a response to increased oxidants, decreased anti-oxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases.
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.
Upstate’s new Nitrotyrosine Assay Kit, Chemiluminescence Detection (Cat. # 17-10006) is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including a pre-coated 96-well plates, Nitrotyrosine peptide standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers.
The assay has a wide dynamic range and high precision, making this assay a valuable new tool for the study of tyrosine nitration.
View All »
Background Information:
Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer and aging. Oxidative stress occurs as a response to increased oxidants, decreased antioxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases.
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.
View All »
RT

Nitrotyrosine ELISA Kit,-20C

货号： 17-10006-2      产品名称： Nitrotyrosine ELISA Kit,-20C   品牌： Millipore 规格： EA 三周到货 生化实验

Nitrotyrosine ELISA
Description:
Nitrotyrosine ELISA
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer, and aging. Oxidative stress occurs as a response to increased oxidants, decreased anti-oxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases.
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.
Upstate’s new Nitrotyrosine Assay Kit, Chemiluminescence Detection (Cat. # 17-10006) is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including a pre-coated 96-well plates, Nitrotyrosine peptide standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers.
The assay has a wide dynamic range and high precision, making this assay a valuable new tool for the study of tyrosine nitration.
View All »
Background Information:
Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer and aging. Oxidative stress occurs as a response to increased oxidants, decreased antioxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases.
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.
View All »
RT