标签归档：mtor

12-645-K   mTOR substrate, 1mg   品牌 Millipore

发表于由

mTOR substrate, 1mg

货号： 12-645-K      产品名称： mTOR substrate, 1mg   品牌： Millipore 规格： MG 三周到货 生化实验

mTOR Substrate
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PRODUCT FAMILY INFORMATION
Kinase & Phosphatase Products
Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Millipore has a broad and continuously expanding portfolio of recombinant proteins. This portfolio includes more than 350 active, inactive and unactivated kinases, phosphatases and the largest available selection of disease relevant mutants. All of our enzymes are available in quantities sufficient for routine testing, as well as high throughput screening.
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Description:
mTOR Substrate
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 mg
Product Overview:
N-terminal 6His-tagged recombinant human mTOR substrate, expressed in E.coli.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
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UniProt Summary:
FUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
SIZE: 2549 amino acids; 288892 Da
SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
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Brand Family:
Upstate
Protein Target:
mTOR (FRAP1)
UniProt Number:
P42345
Target Sub-Family:
Lipid/Atypical
Storage Conditions:
Stable for 1 year at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Packaging:
This product is also available in mg quantities. Please use 12-645-k when ordering.
Gene Symbol:
FRAP1
FRAP
RAPT1
FLJ44809
FRAP2
MTOR
mTOR
RAFT1
View All »
Product Name:
mTOR Substrate
Entrez Gene Number:
NM_004958.2

-80℃

04-385   anti-mTOR, rb mono   品牌 Millipore

发表于由

anti-mTOR, rb mono

货号： 04-385      产品名称： anti-mTOR, rb mono   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-mTOR Antibody, rabbit monoclonal
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REFERENCES
Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR.
Si-meng Chen,Jia-li Liu,Xiang Wang,Chris Liang,Jian Ding,Ling-hua Meng (2012) Biochemical pharmacology.83
Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor.
Van Aller GS, Carson JD, Tang W, Peng H, Zhao L, Copeland RA, Tummino PJ, Luo L (2011) Biochem Biophys Res Commun.406:194-9. Epub 2011 Feb 15.
Safety analysis of the diaphragm in combination with lubricant or acidifying microbicide gels: effects on markers of inflammation and innate immunity in cervicovaginal fluid.
Deborah J Anderson,D’Nyce L Williams,Susan A Ballagh,Kurt Barnhart,Mitchell D Creinin,Daniel R Newman,Frederick P Bowman,Joseph A Politch,Ann C Duerr,Denise J Jamieson (2009) American journal of reproductive immunology (New York, N.Y. : 1989).61
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  FC, ICC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-mTOR Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region in the C-terminus domain of human mTOR.
Molecular Weight:
289 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human mTOR.
Background Information:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
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Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris -20°C

05-1564   Anti-mTOR, mouse monoclonal   品牌 Millipore

发表于由

Anti-mTOR, mouse monoclonal

货号： 05-1564      产品名称： Anti-mTOR, mouse monoclonal   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-mTOR, clone 21A12.2
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-mTOR Antibody, clone 21A12.2
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody only detects mTOR.
Molecular Weight:
~289 kDa
Immunogen:
Full-length human mTOR
Clone:
clone 21A12.2
Isotype:
IgG1κ
Background Information:
mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human. Other species to be tested.
Application Notes:
Confocal Immunofluorescent Immunocytochemistry:
Control:
HEK293 cell lysates
Quality Assurance:
Evaluated by Western Blotting on HEK293 cell lysates.
Western Blotting Analysis: 1:1,000 – 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q6R327
Entrez Gene Number:
NP_004949.1
Gene Symbol:
DKFZp686B11164
KIAA1999
MGC39830
mAVO3
pianissimo
View All »
Alternate Names:
TORC2-specific protein AVO3
rapamycin-insensitive companion of mTOR
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNCTION: Plays an essential role in embryonic growth and development By similarity. Part of the TORC2 complex which plays a critical role in AKT1 ‘Ser-473’ phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
SUBUNIT STRUCTURE: Forms part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. TORC2 does not bind to and is n 2°C – 8°C

05-1564MG   Anti-mTOR, mouse monoclonal   品牌 Millipore

发表于由

Anti-mTOR, mouse monoclonal

货号： 05-1564MG      产品名称： Anti-mTOR, mouse monoclonal   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-mTOR, clone 21A12.2
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-mTOR Antibody, clone 21A12.2
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody only detects mTOR.
Molecular Weight:
~289 kDa
Immunogen:
Full-length human mTOR
Clone:
clone 21A12.2
Isotype:
IgG1κ
Background Information:
mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human. Other species to be tested.
Application Notes:
Confocal Immunofluorescent Immunocytochemistry:
Control:
HEK293 cell lysates
Quality Assurance:
Evaluated by Western Blotting on HEK293 cell lysates.
Western Blotting Analysis: 1:1,000 – 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q6R327
Entrez Gene Number:
NP_004949.1
Gene Symbol:
DKFZp686B11164
KIAA1999
MGC39830
mAVO3
pianissimo
View All »
Alternate Names:
TORC2-specific protein AVO3
rapamycin-insensitive companion of mTOR
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNCTION: Plays an essential role in embryonic growth and development By similarity. Part of the TORC2 complex which plays a critical role in AKT1 ‘Ser-473’ phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
SUBUNIT STRUCTURE: Forms part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. TORC2 does not bind to and is n 2°C – 8°C

05-1592   Anti-mTOR, clone 21D8.2   品牌 Millipore

发表于由

Anti-mTOR, clone 21D8.2

货号： 05-1592      产品名称： Anti-mTOR, clone 21D8.2   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-mTOR, clone 2ID8.2
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100% Performance Guaranteed
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-mTOR Antibody, clone 2ID8.2
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes mTOR.
Molecular Weight:
289 kDa
Immunogen:
Flag-tagged recombinant protein corresponding to residues surrounding mTOR.
Clone:
2ID8.2
Isotype:
IgG1κ
Background Information:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
View All »
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Proven to react with human and mouse.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a previous lot detected mTOR in C2C12, HeLa, and NIH/3T3 cell lysates.
Control:
Hek293 cell lysate
Quality Assurance:
Evaluated by Western Blot in Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on Hek293 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified ascites containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl and 0.05% Sodium Azide.
Storage Conditions:
Maintain refrigerated at 2-8°C for 1 year from date of receipt.
UniProt Number:
P42345
Entrez Gene Number:
NP_004949.1
Gene Symbol:
FLJ44809
FRAP
FRAP1
FRAP2
OTTHUMP00000001983
RAFT1
RAPT1
mTOR
View All »
Alternate Names:
FK506 binding protein 12-rapamycin associated protein 1
FK506 binding protein 12-rapamycin associated protein 2
FK506-binding protein 12-rapamycin complex-associated protein 1
FKBP-rapamycin associated protein
FKBP12-rapamycin complex-associated protein 1
Mammalian target of rapamycin
Rapamycin target protein
mechanistic target of rapamycin (serine/threonine kinase)
rapamycin and FKBP12 target 1
rapamycin associated protein FRAP2
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
The protein encoded by thi 2°C – 8°C

ALX-210-065-C050   mTOR, pAb   品牌 Alexis

发表于由

mTOR, pAb

货号： ALX-210-065-C050      产品名称： mTOR, pAb   品牌： Alexis 规格： 50.micro.g 三周 生化实验

mTOR, pAb
Product Specification
Alternative Name: Mammalian target of rapamycin, FRAP1, RAFT1, RAPT1
Host: Rabbit
Immunogen: Human mTOR (mammalian target of rapamycin) (aa 1223-1290).
UniProt ID: P42345
Application: Immunoprecipitation (1-5µg)
                Western Blot (1µg/ml)
Specificity: Recognizes human and rat mTOR.
Formulation: Liquid. Protein G purified antibody in PBS containing 10% glycerol and 0.02% sodium azide.
Handling: Avoid freeze/thaw cycles.
Long Term Storage: -20°C

-20°C

ALX-215-065-1   mTOR (human FRB Domain), pAb   品牌 Alexis

发表于由

mTOR (human FRB Domain), pAb

货号： ALX-215-065-1      产品名称： mTOR (human FRB Domain), pAb   品牌： Alexis 规格： 1 Vial 三周 生化实验

mTOR (human FRB Domain), pAb
mTOR (FKBP12-rapamycin associated protein (FRAP)) is one of a family of proteins involved in cell cycle progression, DNA recombination,
and DNA damage detection. It has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. FKBP12-rapamycin-binding
(FRB) domain is a conserved 11kDa region necessary for FKBP12-rapamycin binding. FRB is one of several functional domains of mTOR: HEAT
 (Huntington elongation factor 1A-protein phosphatase 2A-A subunit-TOR); FAT (FRAP, ATM, TTRAP2); PIKK (PI3-kinase-related kinase); RD
(regulatory domain); and FATC (FAT, C terminal).
Product Specification
Alternative Name: mTOR (human FKBP12-rapamycin-binding domain)
Host: Rabbit
Immunogen: Recombinant human mTOR (FKBP12-rapamycin-binding (FRB) domain).
UniProt ID: P42345
Application: Chromatin Immunoprecipitation
                Immunocytochemistry
                Western Blot (1:2’000)
Specificity: Recognizes the FRB domain of fusion proteins expressed in Saccharomyces cerevisiae (see H. Haruki, et al. 2008) and the human FRB domain of mTOR.
Formulation: Lyophilized from neat serum containing sodium azide.
Reconstitution: Reconstitute with 50µl water.
Long Term Storage: +4°C

+4°C

07-1415   Anti-mTOR (rabbit polyclonal)   品牌 Millipore

发表于由

Anti-mTOR (rabbit polyclonal)

货号： 07-1415      产品名称： Anti-mTOR (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-mTOR (catalystic domain) Antibody
Recommended Replacement for: 07-1415 is a recommended replacement for AB3882
Species Reactivity Key Applications Host Format Antibody Type
H, R  WB, IP, IH(P) Rabbit Purified Polyclonal Antibody
Description:
Anti-mTOR (catalystic domain) Antibody
Replaces:
07-1415 is a recommended replacement for AB3882
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Recognizes mTOR (mammalian target of rapamycin). Tested reactivity includes human and rat..
Molecular Weight:
Recognizes at 289 kDa band on reducing gels.
Epitope:
1223-1290 aa mTOR
Immunogen:
Recombinant GST fusion protein corresponding to amino acids 1223-1290 of human mTOR.
Isotype:
IgG
Background Information:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GbL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
Species Reactivity:
Human
Rat
Species Reactivity Note:
Reacts with Human and Rat.
Control:
L6 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected mTOR on 10 μg of L6 lysates.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal IgG in PBS containing 10% glycerol with 0.02% sodium azide added as a preservative.
Storage Conditions:
Stable at -20ºC in undiluted aliquots for up to one year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol con -20°C

07-231   ANTI-MTOR (MTAB1) 200UL   品牌 Millipore

发表于由

ANTI-MTOR (MTAB1) 200UL

货号： 07-231      产品名称： ANTI-MTOR (MTAB1) 200UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-mTOR (mTAb1) Antibody
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REFERENCES
Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle.
Vernon G Coffey,Henriette Pilegaard,Andrew P Garnham,Brendan J O’Brien,John A Hawley (2009) Journal of applied physiology (Bethesda, Md. : 1985).106
Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle.
Vernon G Coffey, Donald W Reeder, Graeme I Lancaster, Wee Kian Yeo, Mark A Febbraio, Ben B Yaspelkis, John A Hawley (2007) Medicine and science in sports and exercise.39:2135-44
Attenuation of mammalian target of rapamycin activity by increased cAMP in 3T3-L1 adipocytes.
Scott, P H and Lawrence, J C (1998) J. Biol. Chem., 273: 34496-501 (1998)
Evidence of insulin-stimulated phosphorylation and activation of the mammalian target of rapamycin mediated by a protein kinase B signaling pathway.
Scott, P H, et al. (1998) Proc. Natl. Acad. Sci. U.S.A., 95: 7772-7 (1998)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IP, EA, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-mTOR (mTAb1) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
mTOR is also known as FRAP or RAFT
Specificity:
Recognizes mTOR.
Molecular Weight:
240 kDa
Immunogen:
peptide (C-DTNAKGNKRSRTRTDSYS) corresponding to amino acids 2433-2450 of rat mTOR, mammalian target of rapamycin
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Control:
BDNF activated mTOR in dendritic, neural lysates or synaptoneurosomes
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from 293T cells transfected with mTOR
Purification Method:
Immunoaffinity purified
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P42345
Entrez Gene Number:
NM_004958
Gene Symbol:
FRAP1
RAPT1
MTOR
FRAP
mTOR
FLJ44809
FRAP2
RAFT1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Enzyme Assay
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cy -20℃

2255-500   mTOR Inhibitor, WYE-28   品牌 Biovision

发表于由

mTOR Inhibitor, WYE-28

货号： 2255-500      产品名称： mTOR Inhibitor, WYE-28   品牌： Biovision 规格： 500 μg 3周到货 生化实验

mTOR Inhibitor, WYE-28
A selective, picomolar-potent mTOR inhibitor
Product Overview
Product Name: mTOR Inhibitor, WYE-28
Alternate Name/Synonyms: 4-(6-{4-[3-(4-Hydroxymethyl-phenyl)-ureido]-phenyl}-4-morpholin-4-yl-pyrazolo[3,4-d]pyrimidin-1-yl)-piperidine-1-carboxylic acid methyl ester
Description: Cell-permeable. A potent, selective, and ATP-competitive mTOR (mammalian Target Of Rapamycin) inhibitor (IC₅₀ = 0.08 nM). Displays 75-fold selectivity over PI3Kα (IC₅₀ = 6 nM).
Peptide Sequence: N/A
Appearance: White solid
Formulation: N/A
CAS Number: 1062172-60-4
Molecular Formula: C₃₀H₃₄N₈O₅
Molecular Weight: 586.64
Purity: ≥98% by HPLC
Solubility:DMSO (>60 mg/ml)
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from light and moisture
SMILES: COC(=O)N1CCC(CC1)N2C3=C(C=N2)C(=NC(=N3)C4=CC=C(C=C4)NC(=O)NC5=CC=C(C=C5)CO)N6CCOCC6
InChi: InChI=1S/C30H34N8O5/c1-42-30(41)37-12-10-24(11-13-37)38-28-25(18-31-38)27(36-14-16-43-17-15-36)34-26(35-28)21-4-8-23(9-5-21)33-29(40)32-22-6-2-20(19-39)3-7-22/h2-9,18,24,39H,10-17,19H2,1H3,(H2,32,33,40)
InChi Key: DLIGFKRUAOAIBA-UHFFFAOYSA-N
PubChem CID: 24936050
MDL Number: N/A
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

2255-1000   mTOR Inhibitor, WYE-28   品牌 Biovision

发表于由

mTOR Inhibitor, WYE-28

货号： 2255-1000      产品名称： mTOR Inhibitor, WYE-28   品牌： Biovision 规格： 1 mg 3周到货 生化实验

mTOR Inhibitor, WYE-28
A selective, picomolar-potent mTOR inhibitor
Product Overview
Product Name: mTOR Inhibitor, WYE-28
Alternate Name/Synonyms: 4-(6-{4-[3-(4-Hydroxymethyl-phenyl)-ureido]-phenyl}-4-morpholin-4-yl-pyrazolo[3,4-d]pyrimidin-1-yl)-piperidine-1-carboxylic acid methyl ester
Description: Cell-permeable. A potent, selective, and ATP-competitive mTOR (mammalian Target Of Rapamycin) inhibitor (IC₅₀ = 0.08 nM). Displays 75-fold selectivity over PI3Kα (IC₅₀ = 6 nM).
Peptide Sequence: N/A
Appearance: White solid
Formulation: N/A
CAS Number: 1062172-60-4
Molecular Formula: C₃₀H₃₄N₈O₅
Molecular Weight: 586.64
Purity: ≥98% by HPLC
Solubility:DMSO (>60 mg/ml)
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from light and moisture
SMILES: COC(=O)N1CCC(CC1)N2C3=C(C=N2)C(=NC(=N3)C4=CC=C(C=C4)NC(=O)NC5=CC=C(C=C5)CO)N6CCOCC6
InChi: InChI=1S/C30H34N8O5/c1-42-30(41)37-12-10-24(11-13-37)38-28-25(18-31-38)27(36-14-16-43-17-15-36)34-26(35-28)21-4-8-23(9-5-21)33-29(40)32-22-6-2-20(19-39)3-7-22/h2-9,18,24,39H,10-17,19H2,1H3,(H2,32,33,40)
InChi Key: DLIGFKRUAOAIBA-UHFFFAOYSA-N
PubChem CID: 24936050
MDL Number: N/A
USAGE: For Research Use Only! Not For Use in Humans.

-20°C