标签归档：moesin

07-121   ANTI-MOESIN PC 12-305   品牌 Millipore

发表于由

ANTI-MOESIN PC 12-305

货号： 07-121      产品名称： ANTI-MOESIN PC 12-305   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Moesin Antibody
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REFERENCES
Expression level, subcellular distribution and rho-GDI binding affinity of merlin in comparison with Ezrin/Radixin/Moesin proteins.
Maeda, M, et al. (1999) Oncogene, 18: 4788-97 (1999)
Moesin, like ezrin, colocalizes with actin in the cortical cytoskeleton in cultured cells, but its expression is more variable.
Franck, Z, et al. (1993) J. Cell. Sci., 105 ( Pt 1): 219-31 (1993)
Increased ezrin expression and activation by CDK5 coincident with acquisition of the senescent phenotype
Yang, H. S. and Hinds, P. W. (2003) Mol Cell, 11:1163-76 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-Moesin Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Moesin
Molecular Weight:
78kDa
Immunogen:
peptide corresponding to amino acids 401-413 of human Moesin (C-EALLQASRDQKKT)
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
routinely evaluated by immunoblot on HeLa, A431, Jurkat, HFF, Raji, 3T3, CTLL, L6 or PC-12 cell lysates
Purification Method:
Protein A chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.
Storage Conditions:
2 years at -20°C
UniProt Number:
P26038
Entrez Gene Number:
NM_002444
Gene Symbol:
MSN
Moesin
moesin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
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UniProt Summary:
FUNCTION: SwissProt: P26038 # Probably involved in connections of major cytoskeletal structures to the plasma membrane.
SIZE: 577 amino acids; 67820 Da
SUBUNIT: In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). Binds SLC9A3R1.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity).
TISSUE SPECIFICITY: In all tissues and cultured cells studied.
PTM: Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures (By similarity).
SIMILARITY: SwissProt: P26038 ## Contains 1 FERM domain.
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Brand Family:
Upstate
Product Name:
Anti-Moesin Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Rabbit
Applications:
This Anti-Moesin Antibody is validated for use in WB for the detection of Moesin.

-20°C

AB3843   EZRIN/RADIXIN/MOESIN, RBX-100UL   品牌 Millipore

发表于由

EZRIN/RADIXIN/MOESIN, RBX-100UL

货号： AB3843      产品名称： EZRIN/RADIXIN/MOESIN, RBX-100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Ezrin/Radixin/Moesin Antibody
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REFERENCES
Nuclear reprogramming of somatic cells by embryonic stem cells is affected by cell cycle stage.
Stephen Sullivan, Steve Pells, Martin Hooper, Ed Gallagher, Jim McWhir (2006) Cloning and stem cells.8:174-88
Species Reactivity Key Applications Host Format Antibody Type
H, M, Mk, R  WB, ICC, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Ezrin/Radixin/Moesin Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.
Immunogen:
KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
Species Reactivity:
Human
Mouse
Monkey
Rat
Application Notes:
Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)
Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm
Immunocytochemistry (IF): 1:100
Optimal working dilutions must be determined by the end user.
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Presentation:
Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.
Storage Conditions:
Maintain at -20ºC for 12 months, do not aliquot.
UniProt Number:
O14745
Entrez Gene Number:
NM_004252.2
Gene Symbol:
SLC9A3R1
+exchanger)
NHERF1
+H
NHERF
Na
NHERF-1
EBP50
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: O14745 # Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediat -20℃